Map-based cloning proves qGC-6, a major QTL for gel consistency of japonica/indica cross, responds by Waxy in rice (Oryza sativa L.)

Grasses produce seeds on spikelets, a unique type of inflorescence. Despite the importance of grass crops for food, the genetic mechanisms that control spikelet development remain poorly understood. In this study, we used m34-z, a new mutant allele of the rice (Oryza sativa) E-class gene OsMADS34, to examine OsMADS34 function in determining the identities of glumes (rudimentary glume and sterile lemma) and grain size. In the m34-z mutant, both the rudimentary glume and sterile lemma were homeotically converted to the lemma-like organ and acquired the lemma identity, suggesting that OsMADS34 plays important roles in the development of glumes. In the m34-z mutant, most of the grains from the secondary panicle branches (spb) were decreased in size, compared with grains from wild-type, but no differences were observed in the grains from the primary panicle branches. The amylose content and gel consistency, and a seed-setting rate from the spb were reduced in the m34-z mutant. Interesting, transcriptional activity analysis revealed that OsMADS34 protein was a transcription repressor and it may influence grain yield by suppressing the expressions of BG1, GW8, GW2, and GL7 in the m34-z mutant. These findings revealed that OsMADS34 largely affects grain yield by affecting the size of grains from the secondary branches.

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“…The GC, gelatinization temperature (GT), and AC were determined as previously described (Su et al, 2011). …”

Section: Methodsmentioning

confidence: 99%

Regulatory Role of OsMADS34 in the Determination of Glumes Fate, Grain Yield, and Quality in Rice

et al. 2016

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“…Wx is a pleiotropic gene, which also regulates GC. Map-based cloning has confirmed that Wx is the major QTL qGC-6 for GC of japonica/indica cross (Su et al 2011). More than 20 QTLs for GC have been identified, but only Wx has been cloned.…”

Section: Introductionmentioning

confidence: 90%

Population structure and association analysis of yield and grain quality traits in hybrid rice primal parental lines

et al. 2016

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To make advances in rice molecular design breeding, it is important to understand the genetic background and phenotypes of the parental lines, to known superior allele of the breeding material. In this study, 34 pairs of insertion-deletion (InDel) markers were used to analyze the differentiation of indica-japonica properties in 277 primal parental lines of hybrid rice that are used in China and abroad. The genetic diversity and population structure were assessed with simple sequence repeat (SSR) markers. We also evaluated the association between SSR markers and seven yield traits and four grainquality traits of 256 indica rice lines. The main results are as follows. (1) Euphytica (2016) 212:261-273 DOI 10.1007/s10681-016-1766 eight for grain width, eight for grain length/width ratio, one for thousand grain weight, one for amylose content, two for gel consistency, one for gelatinization temperature and one for total protein content. (3) Among those loci, 16 markers were closely linked to previously identified genes. Superior allele carrier lines and allele effects were also analyzed. Our results provided the population structure and indica-japonica properties for parent matching, and the marker-trait association will also be useful for molecular design breeding.

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“…The study of Tian et al (2010) demonstrated that not only AC and GC were controlled by Wx gene, but also GT was influenced to some extent. Through positional cloning, it was found that GC was determined by Wx gene (Su et al, 2011). In this study, from the genetic rule of quality traits in different substitution lines, it was further proved that Wx gene had pleiotropies and there was negative correlation between AC and GC.…”

Section: Discussionmentioning

confidence: 57%

Evaluation of genetic effect nphysiochemical properties changes of Wx near isogenic lines of Y58S in rice

Sheng

Zhou

et al. 2015

Chilean J. Agric. Res.

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The Wx gene primarily influences the physiochemical properties changes of rice (Oryza sativa L.) To improve the grain qualities of the cv. Y58S with low amylose content (AC) value, the genetic effect of Wx gene was explored on quality traits. With the BC3F2 genetic population of Y58S line associated with different AC value as the materials, this study reported the genetic effect of three different Wx alleles (Wx ) under near-isogenic background. It was shown that Wx had major effects on the quality traits, and the genetic effect value was in the order Wx a > Wx in > Wx b . In addition, during the course of physiochemical properties changes of higher AC rice cultivars determined by Wx a or Wx in allele, accompanied by the increasing of AC, gel consistency and head milled rice recovery would be decreased in combination with the rising of chalky traits, all of which resulted in substantial fluctuations on quality traits (p < 0.01). However, the genetic law was not evident for Wx b allele. Therefore, except for the major gene Wx, the minor genes in the regulative network of starchsynthesis might be utilized for quality improvement.

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Map-based cloning proves qGC-6, a major QTL for gel consistency of japonica/indica cross, responds by Waxy in rice (Oryza sativa L.)

Cited by 76 publications

References 34 publications

Regulatory Role of OsMADS34 in the Determination of Glumes Fate, Grain Yield, and Quality in Rice

Regulatory Role of OsMADS34 in the Determination of Glumes Fate, Grain Yield, and Quality in Rice

Population structure and association analysis of yield and grain quality traits in hybrid rice primal parental lines

Evaluation of genetic effect nphysiochemical properties changes of Wx near isogenic lines of Y58S in rice

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