Map‐based cloning and functional analysis revealed ABCC2 is responsible for Cry1Ac toxin resistance inBombyx mori

Abstract

Bt toxins are parasporal crystals produced by Bacillus thuringiensis (Bt). They have specific killing activity against various insects and have been widely used to control agricultural pests. However, their widespread use has developed the resistance of many target insects. To maintain the sustainable use of Bt products, the resistance mechanism of insects to Bt toxins must be fully clarified. In this study, Bt-resistant and Bt-susceptible silkworm strains were used to construct genetic populations, and the genetic pattern of silkworm resistance to Cry1Ac toxin was determined. Sequence-tagged site molecular marker technology was used to finely map the resistance gene and to draw a molecular genetic linkage map, and the two closest markers were T1590 and T1581, indicating the resistance gene located in the 155 kb genetic region. After analyzing the sequence of the predicted gene in the genetic region, an ATP binding cassette transporter (ABCC2) was identified as the candidate gene. Molecular modeling and protein-protein docking result showed that a tyrosine insertion in the mutant ABCC2 might be responsible for the interaction between Cry1Ac and ABCC2. Moreover, CRISPR/Cas9-mediated genome editing technology was

The molecular basis of pine wilt disease resistance inPinus massoniana

“…The common response of P. massoniana included the ABC transporter, arginine, and proline pathways. First, ABC transporters were involved in the transport of secondary metabolites and plant hormones, detoxification, and signal transduction, which indirectly aided resistance to PWN (Wang et al, 2022; Li et al, 2024; Zhou et al, 2024). Second, arginine and proline indirectly affected the reproduction and pathogenicity of the PWN by promoting plant growth and development, enhancing stress resistance, acting as antioxidant defense molecules, participating in defense responses, and regulating metabolic pathways (Zhang et al, 2022; Theisen et al, 2024; Wang et al, 2024b).…”

Metabonomic Analysis of Silkworm Midgut Reveals Differences between the Physiological Effects of an Artificial and Mulberry Leaf Diet

“…The ABC transporter belongs to a family of membrane proteins [22], and animal members of the ABC family were first found in the human genome [23], and, subsequently, in all animals [24]. In insects, including Bombyx mori, midgut ABC transporters play a role in uric acid metabolism and resistance to insecticides [25][26][27][28], and studies have shown that the ABC transporter specific to the silkworm midgut is related to the response of silkworm molting hormone responses (20-E) [29]. In silkworms of the same sex, the midgut metabolites in individuals fed on an AD and ML were enriched in the ABC transporter signaling pathway and no differences between the sexes were observed, while the intestinal metabolites of silkworms fed on the same diet showed no ABC transporter enrichment (Supplementary Figure S4).…”