Map-based cloning and characterization of<i>BPH29</i>, a B3 domain-containing recessive gene conferring brown planthopper resistance in rice

Wang, Ying; Cao, Liming; Zhang, Yuexiong; Cao, Changxiang; Liu, Fang; Huang, Fang; Qiu, Yongfu; Li, Rongbai; Lou, Xiaojin

doi:10.1093/jxb/erv318

Cited by 148 publications

(114 citation statements)

References 63 publications

(70 reference statements)

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“…Since the first reports of the BPH resistance genes Bph1 and bph2 by Athwal et al 8, at least 30 new BPH resistance loci have been identified from cultivated and wild species of Oryza 79101112131415161718. To date, four of these resistance genes, Bph14 19, BPH26 11, Bph3 20 and BPH29 12, have been cloned. Bph14 and BPH26 both encode a coiled-coil, nucleotide-binding-site, leucine-rich repeat (CC-NBS-LRR) protein1921.…”

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confidence: 99%

“…Bph14 and BPH26 both encode a coiled-coil, nucleotide-binding-site, leucine-rich repeat (CC-NBS-LRR) protein1921. BPH29 contains a B3 DNA-binding domain12. Three BPH resistance genes were inferred to activate the salicylic acid signaling pathway111219.…”

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confidence: 99%

“…BPH29 contains a B3 DNA-binding domain12. Three BPH resistance genes were inferred to activate the salicylic acid signaling pathway111219. Bph3 is a cluster of three BPH resistance genes encoding plasma membrane-localized lectin receptor kinases (OsLecPK1- OsLecPK3)20.…”

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confidence: 99%

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Bph32, a novel gene encoding an unknown SCR domain-containing protein, confers resistance against the brown planthopper in rice

Ren

Gao

et al. 2016

Sci Rep

128

131

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An urgent need exists to identify more brown planthopper (Nilaparvata lugens Stål, BPH) resistance genes, which will allow the development of rice varieties with resistance to BPH to counteract the increased incidence of this pest species. Here, using bioinformatics and DNA sequencing approaches, we identified a novel BPH resistance gene, LOC_Os06g03240 (MSU LOCUS ID), from the rice variety Ptb33 in the interval between the markers RM19291 and RM8072 on the short arm of chromosome 6, where a gene for resistance to BPH was mapped by Jirapong Jairin et al. and renamed as “Bph32”. This gene encodes a unique short consensus repeat (SCR) domain protein. Sequence comparison revealed that the Bph32 gene shares 100% sequence identity with its allele in Oryza latifolia. The transgenic introgression of Bph32 into a susceptible rice variety significantly improved resistance to BPH. Expression analysis revealed that Bph32 was highly expressed in the leaf sheaths, where BPH primarily settles and feeds, at 2 and 24 h after BPH infestation, suggesting that Bph32 may inhibit feeding in BPH. Western blotting revealed the presence of Pph (Ptb33) and Tph (TN1) proteins using a Penta-His antibody, and both proteins were insoluble. This study provides information regarding a valuable gene for rice defence against insect pests.

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Bph32, a novel gene encoding an unknown SCR domain-containing protein, confers resistance against the brown planthopper in rice

Ren

Gao

et al. 2016

Sci Rep

128

131

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“…Genetic resistance against all the three species has been reported through large scale screening of rice germplasm under greenhouse conditions (Heinrichs et al 1985, Bentur et al 2011. Such a screening method has mainly identified genotypes that are less preferred and are less fed upon (Horgan, 2009 glaberrima (Cheng et al 2013, Fujita et al 2013, Wang et al 2015. These studies have shown that though resistance to planthoppers is wide spread and distributed widely among Oryza species, resistance is quantitative.…”

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confidence: 99%

Rice–gall midge interactions: Battle for survival

Bentur

Rawat

Divya

et al. 2016

Journal of Insect Physiology

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“…Furthermore, overuse of insecticides may affect populations of natural enemies of BPH and lead to resistance/tolerance to insecticides, and resurgence of the BPH problem7. Therefore, breeding for host resistance is considered the most economical and environmentally friendly strategy for BPH control8.…”

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Development and evaluation of near-isogenic lines for brown planthopper resistance in rice cv. 9311

Xiao

et al. 2016

Sci Rep

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Brown planthopper (BPH) is the most destructive pest of rice in Asia. To date 29 BPH resistance genes have been identified, but only a few genes are being used in breeding due to inefficient markers for marker-assisted selection (MAS) and little knowledge of the real effects of the genes. In this study we individually transferred 13 genes or QTLs (Bph14, QBph3, QBph4, Bph17, Bph15, Bph20, Bph24, Bph6, Bph3, Bph9, Bph10, Bph18 and Bph21) into cultivar 9311 by marker assisted backcross breeding (MABB). Through positive and negative selection we narrowed the segments from donors containing Bph14, Bph15, Bph6 and Bph9 to 100–400 kb. Whole-genome background selection based on a high resolution SNP array was performed to maximize reconstitution of the recurrent parent genome (RPG 99.2–99.9%). All genes reduced BPH growth and development and showed antibiotic responses in seedlings. Based on genetic effects and amino acid sequences of genes in three clusters we inferred that Bph10 and Bph21 might be identical to Bph26, whereas Bph9 and Bph18 were different. Bph15 might be same with Bph17, but QBph4, Bph20 and Bph24 might be different. We believe that these NILs will be useful in rice BPH resistance research and breeding.

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Map-based cloning and characterization ofBPH29, a B3 domain-containing recessive gene conferring brown planthopper resistance in rice

Abstract: HighlightA brown planthopper resistance recessive gene, BPH29, was cloned which contained a B3 DNA-binding domain and conferred resistance by a mechanism that was similar to plant defence against pathogens.

Cited by 148 publications

References 63 publications

Bph32, a novel gene encoding an unknown SCR domain-containing protein, confers resistance against the brown planthopper in rice

Bph32, a novel gene encoding an unknown SCR domain-containing protein, confers resistance against the brown planthopper in rice

Rice–gall midge interactions: Battle for survival

Development and evaluation of near-isogenic lines for brown planthopper resistance in rice cv. 9311

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