Manufacturing of poly(ethylene glycol diacrylate)-based hollow microvessels using microfluidics

Aykar, Saurabh S.; Reynolds, David E.; McNamara, Marilyn C.; Hashemi, Nastaran

doi:10.1039/c9ra10264g

Cited by 21 publications

(23 citation statements)

References 52 publications

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“…[28] Changing the geometry and number of inlets can drastically affect the shape and size of the resulting fibers. [8, 29, 30, 31] During fiber fabrication, both core (pre-gel) and sheath (gellator) solutions are used; the core solution will be crosslinked into the resulting microfiber, while the sheath solution helps guide the core solution through the channel, further shaping it and preventing clogging. [6, 30, 32, 33] The viscosities and flow rates used can also affect the shape, size, topography, and mechanical properties of the resulting fibers.…”

Section: Resultsmentioning

confidence: 99%

Behavior of Neural Cells Post Manufacturing and After Prolonged Encapsulation within Conductive Graphene-Laden Alginate Microfibers

McNamara

Asli²,

Pemathilaka³

et al. 2021

Preprint

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Engineering conductive 3D cell scaffoldings offer unique advantages towards the creation of physiologically relevant platforms with integrated real-time sensing capabilities. Toward this goal, rat dopaminergic neural cells were encapsulated into graphene-laden alginate microfibers using a microfluidic fiber fabrication approach, which is unmatched for creating continuous, highly tunable microfibers. Incorporating graphene increases the conductivity of the alginate microfibers 148%, creating a similar conductivity to native brain tissue. Graphene leads to an increase in the cross-sectional sizes and porosities of the fibers, while reducing the roughness of the fiber surface. The cell encapsulation procedure has an efficiency rate of 50%, and of those cells, approximately 30% remain for the entire 6-day observation period. To understand how encapsulation effects cell genetics, the genes IL-1β, TH, TNF-α, and TUBB-3 are analyzed, both after manufacturing and after encapsulation for six days. The manufacturing process and combination with alginate leads to an upregulation of TH, and the introduction of graphene further increases its levels; however, the inverse trend is true of TUBB-3. Long-term encapsulation shows continued upregulation of TH and of TNF-α, and six-day exposure to graphene leads to the upregulation of TUBB-3 and IL-1β, which indicates increased inflammation.

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Section: Resultsmentioning

confidence: 99%

Behavior of Neural Cells Post Manufacturing and After Prolonged Encapsulation within Conductive Graphene-Laden Alginate Microfibers

McNamara

Asli²,

Pemathilaka³

et al. 2021

Preprint

Self Cite

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“…MSCs and adult rat hippocampal stem/progenitor cells were cultured on the surface of PEGDA fibers, and showed good cell adhesion and proliferation, as well as ECM deposition. Based on the laminar flow characteristics of PEGDA and PEG in microfluidic devices, Aykar et al [135] further broadened the function of PEGDA fibers by developing hollow microfluidic microvessels. To form the hollow structure, the PEG solution was injected into the core and sheath flow channels, while PEGDA was used as the shell flow.…”

Section: Pegdamentioning

confidence: 99%

Biomaterials for microfluidic technology

Chen

Zhang

et al. 2022

Mater. Futures

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Micro/nanomaterial-based drug and cell delivery systems play an important role in biomedical fields for their injectability and targeting. Microfluidics is a rapidly developing technology and has become a robust tool for preparing biomaterial micro/nanocarriers with precise structural control and high reproducibility. By flexibly designing microfluidic channels and manipulating fluid behavior, various forms of biomaterial carriers can be fabricated using microfluidics, including microspheres, nanoparticles and microfibers. In this review, recent advances in biomaterials for designing functional microfluidic vehicles are summarized. We introduce the application of natural materials such as polysaccharides and proteins as well as synthetic polymers in the production of microfluidic carriers. How the material properties determine the manufacture of carriers and the type of cargoes to be encapsulated is highlighted. Furthermore, the current limitations of microfluidic biomaterial carriers and perspectives on its future developments is presented.

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“…According to our previous report [65], this five-channel microfluidic device used in this study to fabricate Alginate hollow microfibers, was manufactured from 6.0 mm thickness Poly (Methyl Methacrylate) (PMMA, Grainger, IL, US). A Computerized Numerical Control (CNC) mini-mill (Minitech Machinery Corporation, Norcross, GA) was used to mill the core channels and the two independent chevron chevrons with a dimension of 1.00 mm × 0.75 mm (width × height) and 0.375 mm × 0.25 mm (width × height), respectively.…”

Section: Fabrication Of Microfluidic Devicesmentioning

confidence: 99%

Polyethylene Glycol Wrapped Alginate/Graphene Hollow Microfibers as Flexible Supercapacitors

Nasirian

Niaraki-Asli

Aykar

et al. 2021

Preprint

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Carbon-modified fibrous structures with high biocompatibility have attracted much attention as supercapacitors due to their low cost, sustainability, abundance, and excellent electrochemical performance. However, some of these carbon-based materials suffer from low specific capacitance and electrochemical performance, which have been significant challenges in developing biocompatible electronic devices. In this regard, several studies have been reported on the development of 3D carbon-based micro architectures that provided high conductivity, energy storage potential, and 3D porosity frameworks. This study reports manufacturing of microfluidic Alginate hollow microﬁber modified by water-soluble modified Graphene (BSA-Graphene). These architectures successfully exhibited conductivity enhancement conductivity of about 20 times more compared to Alginate hollow microfibers, and without any signiﬁcant change in the inner-dimension values of hollow region (220.0 ± 10.0 µm) in comparison with pure alginate hollow microfibers. In the presence of Graphene, more obtained specific surface permeability and active ion adsorption sites could successfully provide as shorter pathways. These obtained continuous ion transport networks resulted in improved electrochemical performance. These desired electrochemical properties of the microfibers make Alginate/Graphene hollow fibers an excellent choice for further use in the development of lightweight flexible supercapacitors with scalable potential to be used in intelligent health electronic gadgets.

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Manufacturing of poly(ethylene glycol diacrylate)-based hollow microvessels using microfluidics

Abstract: Biocompatible and self-standing poly(ethylene glycol diacrylate)-based hollow microvessels were fabricated from a microfluidic device using microfluidic principles.

Cited by 21 publications

References 52 publications

Behavior of Neural Cells Post Manufacturing and After Prolonged Encapsulation within Conductive Graphene-Laden Alginate Microfibers

Behavior of Neural Cells Post Manufacturing and After Prolonged Encapsulation within Conductive Graphene-Laden Alginate Microfibers

Biomaterials for microfluidic technology

Polyethylene Glycol Wrapped Alginate/Graphene Hollow Microfibers as Flexible Supercapacitors

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