Manufacturing bio-bricks using microbial induced calcium carbonate precipitation and human urine

Lambert, Sandrine; Randall, D.G.

doi:10.1016/j.watres.2019.05.069

Cited by 85 publications

(42 citation statements)

References 26 publications

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“…Indeed, they obtained bio-bricks with an optimum compressive strength of 2.2 MPa and a calcite content of 3.4% after 27 days. Other biomineralization studies showed maximum mechanical compressive strengths for bio-bricks of 0.9 MPa [23], 0.9 to 1.1 MPa [37], 2.7 MPa [38] but no D:…”

Section: Assessment Of Biomineralization Of Sporosarcina Pasteurii Dsmentioning

confidence: 98%

“…Composition of the culture medium is one of the factors that can influence the mineral type produced by biomineralization [27], which could explain why two allotropic forms of CaCO 3 could be obtained with the same bacterial strain in two different media. However, several studies demonstrated that Sporosarcina pasteurii produced calcite in similar conditions (sand, cementation medium, mould) with different experimental duration [35][36][37][38].…”

Section: Assessment Of Biomineralization Of Sporosarcina Pasteurii Dsmentioning

confidence: 99%

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Biomineralization of calcium carbonate by marine bacterial strains isolated from calcareous deposits

Vincent

Sabot

Lanneluc

et al. 2020

Matériaux & Techniques

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Biomineralization induced by microbial enzymes, which catalyse CaCO3 precipitation, is a promising field of research for various applications in building eco-materials. Especially, this could provide an eco-friendly process for protection of coastal areas against erosion. In the present investigation, fourteen bacterial strains were isolated and characterized from both natural seawater and calcareous deposits formed on a cathodically protected steel mesh in marine environment. All of them induced calcium carbonate precipitation in various media by producing urease and/or carbonic anhydrase enzymes. The calcium carbonate minerals produced by bacteria were identified by microscopy and µ-Raman spectroscopy. In parallel, an experimental set-up, based on a column reactor, was developed to study biomineralization and microbial capacity of Sporosarcina pasteurii to form sandy agglomerate. These well-known calcifying bacteria degraded the urea present in liquid medium circulating through the column to produce calcium carbonate, which acted as cement between sand particles. The bio-bricks obtained after 3 weeks had a compressive strength of 4.2 MPa. 20% of the inter-granular voids were filled by calcite and corresponded to 13% of the total mass. We successfully showed that bio-column system can be used to evaluate the bacterial ability to agglomerate a sandy matrix with CaCO3.

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Section: Assessment Of Biomineralization Of Sporosarcina Pasteurii Dsmentioning

confidence: 98%

Section: Assessment Of Biomineralization Of Sporosarcina Pasteurii Dsmentioning

confidence: 99%

Biomineralization of calcium carbonate by marine bacterial strains isolated from calcareous deposits

Vincent

Sabot

Lanneluc

et al. 2020

Matériaux & Techniques

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“…Recently, S.E. Lambert et al [16] reported successful manufacture of bio-bricks having compressive strength of 2.7 MPa by incorporating human urine as a source for urea and calcium. This study once more emphasized the environmental friendly and energy efficient aspect of MICP application.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of microbially Induced calcite precipitation (MICP) methods on different soil types for wind erosion control

Chae

Chung

Nam

2020

Environmental Engineering Research

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Application methods (i.e., pouring and mixing method) of Microbially Induced Calcite Precipitation (MICP) and its effect on wind erosion were investigated on four soil types (i.e., medium sand, fine sand, loamy fine sand and loam). With mixing method, calcite precipitated evenly throughout the upper part (0 - 5 cm) of all the soils tested, but with pouring method, only medium sand showed even calcite distribution. The reason can be ascribed to the limited permeability of MICP-inducing solution (i.e., calcium, urea and <i>Sporosarcina pasteurii</i>) through loamy fine sand and loam due to their low hydraulic conductivity (i.e., < 10<sup>-5</sup> cm/s). Moreover, bacterial penetrability was also reduced by calcium (i.e., 70 to 20%) in fine sand. Hence, pouring method for medium sand and mixing method for the others were applied with various MICP-inducing solution concentrations (i.e., 0.1 to 1 M of urea and calcium). When exposed to wind of 15 m/s after MICP application, 0.25 M solution in medium and fine sand, and 0.1 M solution in loamy fine sand and loam showed little or no soil loss. The results suggest that a proper application method be chosen considering soil properties that affect even calcite distribution to mitigate soil erosion.

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“…Improvement in concrete strength and durability of construction material was reported via ureolysis mediated calcium carbonate precipitation with incubation of Bacillus megaterium [23]. Of late, Lambert and Randall [24] has shown that the green brick can be manufactured using human urine. Urine is a rich source of urea and contains nutrients like nitrogen, potassium and phosphorus too [25].…”

Section: Introductionmentioning

confidence: 99%

Microbially induced calcite precipitation using Bacillus velezensis with guar gum

et al. 2020

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Mineral precipitation via microbial activity is a well-known process with applications in various fields. This relevance of microbially induced calcite precipitation (MICP) has pushed researchers to explore various naturally occurring MICP capable bacterial strains. The present study was performed to explore the efficiency of microbially induced calcite precipitation (MICP) via locally isolated bacterial strains and role of guar gum, which is a naturally occurring polymer, on the MICP process. The strains were isolated from local soil and screened for urease activity Further, the urease positive strain was subjected to urea and calcium chloride based medium to investigate the efficacy of isolated strain for microbial induced precipitation. Among screened isolates, the soil bacterium that showed urease positive behaviour and precipitated calcium carbonate was subjected to 16S rRNA gene sequencing. This strain was identified as Bacillus velezensis. Guar gum-a natural polymer, was used as a sole carbon source to enhance the MICP process. It was observed that the isolated strain was able to breakdown the guar gum into simple sugars resulting in twofold increase in calcium carbonate precipitate. Major biochemical activities of isolated strain pertaining to MICP such as ammonium ion concentration, pH profiling, and total reducing sugar with time were explored under four different concentrations of guar gum (0.25%, 0.5%, 0.75% and 1% w/v). Maximum ammonium ion concentration (17.5 μg/ml) and increased pH was observed with 1% guar gum supplementation, which confirms augmented MICP activity of the bacterial strain. Microstructural analysis of microbial precipitation was performed using scanning electron microscopy (SEM) and X-ray diffraction (XRD) techniques, which confirmed the presence of calcium carbonate in different phases. Further, XRD and SEM based studies corroborated that guar gum supplemented media showed significant increase in stable calcite phase as compared to media without guar gum supplementation. Significant diverse group of nitrogenous compounds were observed in guar gum supplemented medium when subjected to Gas Chromatography-Mass spectrometry (GC-MS) profiling.

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Manufacturing bio-bricks using microbial induced calcium carbonate precipitation and human urine

Cited by 85 publications

References 26 publications

Biomineralization of calcium carbonate by marine bacterial strains isolated from calcareous deposits

Biomineralization of calcium carbonate by marine bacterial strains isolated from calcareous deposits

Evaluation of microbially Induced calcite precipitation (MICP) methods on different soil types for wind erosion control

Microbially induced calcite precipitation using Bacillus velezensis with guar gum

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