Manufacturing and structural analysis of antimicrobial kefiran/polyethylene oxide nanofibers for food packaging

Jenab, Anahita; Roghanian, Rasoul; Emtiazi, Giti; Ghaedi, Kamran

doi:10.1007/s13726-016-0496-7

Cited by 21 publications

(10 citation statements)

References 21 publications

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“…After incubation, kefir grains were separated from the fermented product by a plastic sieve, and then the solvent collected (crude kefiran). 16 Samples were kept in sterilized distilled water at 100 ºC for an hour to inactivate hydrolyzing enzymes and dissolve the polysaccharide. Then, samples were centrifuged at 1180g in 20ºC for 15 mins to remove all the cells from the sample (boiled kefiran).…”

Section: Extraction Of Kefiran From Kefir Grainsmentioning

confidence: 99%

“…Finally, the obtained sample was lyophilized after dissolving the sediment in hot distilled water (pure kefiran). 15,16,22 This sample also was stored for the quantitation of IL-6.…”

Section: Extraction Of Kefiran From Kefir Grainsmentioning

confidence: 99%

“…However, so far there are only two reports about kefiran electrospun nanofibers. 16,23 Esnaashari et al (2014) showed fabrication of kefiran electrospun nanofiber by using water as the solvent. 23 But, production of this fiber in water was impossible in our experiment.…”

Section: Cell-scaffolds Interaction and Metabolic Activitiesmentioning

confidence: 99%

“…14,15 Antimicrobial and anti-inflammatory properties of kefir for the treatment of patients infected with one or more strains of resistant microorganism were used. 16 Kefir helps to regulate the immune system of the gastrointestinal tract (GIT). Also, it protects epithelial cells against Bacillus cereus extracellular factors.…”

Section: Introductionmentioning

confidence: 99%

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<p>The Efficacy of Electrospun PAN/Kefiran Nanofiber and Kefir in Mammalian Cell Culture: Promotion of PC12 Cell Growth, Anti-MCF7 Breast Cancer Cells Activities, and Cytokine Production of PBMC</p>

Jenab

Roghanian

Ghorbani

et al. 2020

IJN

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Background: Kefiran is a useful polysaccharide made of branched glucogalactose which is produced by microorganisms. Here the anti-MCF-7 breast cancer cells activity of kefiran and cytokine productions (IL-6) of peripheral blood mononuclear cells (PBMC) treated by kefiran was studied. Also, the effect of using kefiran as a useful and cost-effective scaffold in neural stem cell culture (PC12 cell culture) was investigated. Material and Methods: Kefiran was produced from raw milk with 0.5% fat and 10 g of kefir grains. After incubation for 48 hrs at room temperature, the solvent collected (crude kefiran). These samples were kept at 100°C for 1 hr (boiled kefiran) and the supernatant was precipitated by ethanol (pure kefiran). Then, the electrospun nanofibers, pure polyacrylonitrile (PAN), PAN/kefiran 5%, and PAN/kefiran 10% were fabricated and used as scaffolds in the cell culture. The structure of fabricated was studied by SEM and the cytokine production (IL-6) in vitro in the cell culture supernatant of PBMC line after treatment with kefiran (1mg/mL, 5 mg/mL) and kefiran-PAN 5% and 10% were carried out by enzyme-linked immunosorbent assay (ELISA). The attachment of PC12 cells was examined by inverted microscope. Also, cytotoxicity of kefiran for PC12 and MCF7 cells and morphological changes of PC12 cells were evaluated by MTT and Cresyl violet staining (Nissl staining) respectively. Results: The mean diameter of fabricated PAN/kefiran 5% and 10% nanofibers were 310.2±43.97 nm. The contact angle measurement results (26.9± 1.9 for the pure PAN scaffold vs 12.3± 1.13 for the PAN/kefiran) revealed enhanced hydrophilicity of scaffolds upon the incorporation of kefiran and PAN. Seeding of PC12 cells on the scaffolds showed that fabrication of kefiran into PAN led to the enhancement of cell attachment, proliferation, and morphological changes. Also, the promotion of PBMC growth and decreasing of MCF7 cell lines viability were shown through MTT assay. No significant changes were measured for the level of IL-6 in PAN/kefiran 5% treated cells compared to the control (p ≥ 0.05). Conclusion: These results suggest superior properties of kefiran/PAN nanofibrous scaffolds for the neural stem cell culture especially for repairing injured spinal cord. Also, the pure kefiran could be used for the enhancement of PBMC growth and reducing the MCF7 cancerous cells growth. So, using biocompatible, anti-bacterial, and anti-tumor kefiran/ PAN nanofibers for regenerative medicine seems promising.

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Section: Extraction Of Kefiran From Kefir Grainsmentioning

confidence: 99%

“…Finally, the obtained sample was lyophilized after dissolving the sediment in hot distilled water (pure kefiran). 15,16,22 This sample also was stored for the quantitation of IL-6.…”

Section: Extraction Of Kefiran From Kefir Grainsmentioning

confidence: 99%

Section: Cell-scaffolds Interaction and Metabolic Activitiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

<p>The Efficacy of Electrospun PAN/Kefiran Nanofiber and Kefir in Mammalian Cell Culture: Promotion of PC12 Cell Growth, Anti-MCF7 Breast Cancer Cells Activities, and Cytokine Production of PBMC</p>

Jenab

Roghanian

Ghorbani

et al. 2020

IJN

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“…The role of carrier polymer is to adjust the viscosity of the solution used for fabrication of the electrospun nanofibers . Some of the commonly used polymers as carrier medium are poly(vinyl pyrrolidone) (PVP), poly(vinyl acetate), poly(ethylene oxide), polyacrylonitrile, and poly(vinyl alcohol) …”

Section: Introductionmentioning

confidence: 99%

Morphology enhancement of TiO₂/PVP composite nanofibers based on solution viscosity and processing parameters of electrospinning method

Sadeghi

Vaezi

Kazemzadeh

et al. 2018

J of Applied Polymer Sci

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In this work, different sol solutions with various titanium tetraisopropoxide (TIP)/glacial acetic acid ratios in 2‐propanol with 5 wt % poly(vinyl pyrrolidone) (PVP) (Mw = 360,000 g/mol) were prepared and electrospun. Composition of the prepared sols and as‐spun TiO2/PVP nanofibers were determined by Fourier transform infrared and Raman spectroscopy methods. Morphology of the electrospun TiO2/PVP nanofibers was studied by scanning electron microscopy and transmission electron microscopy (TEM) techniques. Rheometry measurements of the sol solutions showed decrease of viscosity upon the addition of TIP to the polymer solutions with constant polymer and acid concentrations. The sol solution having the lowest viscosity (at shear rate 10 s−1) but the highest TIP/glacial acetic acid ratio showed beaded nanofibers morphology when electrospun under 10 and 12 kV applied voltage while injection rate, needle tip to collector distance, and needle gauge were kept constant. However, smooth electrospun TiO2/PVP composite nanofibers with the average nanofibers diameters (148 ± 79 nm) were achieved under the same condition when applied voltage increased to 15 kV. TEM micrographs of the electrospun TiO2/PVP nanofiber showed that the TiO2 particles with continuous structure are formed at the middle of the nanofiber and distributed along its axis. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018, 135, 46337.

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Kefiran in Tissue Engineering and Regenerative Medicine

Correia¹,

Oliveira²,

Radhouani³

et al. 2021

Polysaccharides of Microbial Origin

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Manufacturing and structural analysis of antimicrobial kefiran/polyethylene oxide nanofibers for food packaging

Cited by 21 publications

References 21 publications

<p>The Efficacy of Electrospun PAN/Kefiran Nanofiber and Kefir in Mammalian Cell Culture: Promotion of PC12 Cell Growth, Anti-MCF7 Breast Cancer Cells Activities, and Cytokine Production of PBMC</p>

<p>The Efficacy of Electrospun PAN/Kefiran Nanofiber and Kefir in Mammalian Cell Culture: Promotion of PC12 Cell Growth, Anti-MCF7 Breast Cancer Cells Activities, and Cytokine Production of PBMC</p>

Morphology enhancement of TiO₂/PVP composite nanofibers based on solution viscosity and processing parameters of electrospinning method

Kefiran in Tissue Engineering and Regenerative Medicine

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Manufacturing and structural analysis of antimicrobial kefiran/polyethylene oxide nanofibers for food packaging

Cited by 21 publications

References 21 publications

<p>The Efficacy of Electrospun PAN/Kefiran Nanofiber and Kefir in Mammalian Cell Culture: Promotion of PC12 Cell Growth, Anti-MCF7 Breast Cancer Cells Activities, and Cytokine Production of PBMC</p>

<p>The Efficacy of Electrospun PAN/Kefiran Nanofiber and Kefir in Mammalian Cell Culture: Promotion of PC12 Cell Growth, Anti-MCF7 Breast Cancer Cells Activities, and Cytokine Production of PBMC</p>

Morphology enhancement of TiO2/PVP composite nanofibers based on solution viscosity and processing parameters of electrospinning method

Kefiran in Tissue Engineering and Regenerative Medicine

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Morphology enhancement of TiO₂/PVP composite nanofibers based on solution viscosity and processing parameters of electrospinning method