Manufacture of elastic biodegradable PLCL scaffolds for mechano-active vascular tissue engineering

Jeong, Sunho; Kim, Soo Hyun; Kim, Young H. A.; Jung, Youngmee; Kwon, Jae Hyun; Kim, Byung Soo; Lee, Young Moo

doi:10.1163/156856204323046906

Cited by 166 publications

(92 citation statements)

References 31 publications

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“…Furthermore, the ideal biomaterial should be capable of being safely replaced by newly formed tissue and also degrade appropriate time period without producing any toxic products [8] . Naturally derived materials used in experimental or clinical treatment of infarcts include tumor-derived basement membrane matrix gel (matrigel) [9] , alginate [10] , collagen [11] , laminin [12] , fibrin [13] and decellularized extracellular matrix (ECM) [14] , all of which can enhance cell and tissue function in the myocardial region.…”

Section: Introductionmentioning

confidence: 99%

Cardiogenic differentiation of mesenchymal stem cells on elastomeric poly (glycerol sebacate)/collagen core/shell fibers

Ravichandran¹

2013

WJC

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AIM:To facilitate engineering of suitable biomaterials to meet the challenges associated with myocardial infarction. METHODS:Poly (glycerol sebacate)/collagen (PGS/ collagen) core/shell fibers were fabricated by core/ shell electrospinning technique, with core as PGS and shell as collagen polymer; and the scaffolds were characterized by scanning electron microscope (SEM), fourier transform infrared spectroscopy (FTIR), contact angle and tensile testing for cardiac tissue engineering. Collagen nanofibers were also fabricated by electrospinning for comparison with core/shell fibers. Studies on cell-scaffold interaction were carried out using cardiac cells and mesenchymal stem cells (MSCs) co-culture system with cardiac cells and MSCs separately serving as positive and negative controls respectively. The co-culture system was characterized for cell proliferation and differentiation of MSCs into cardiomyogenic lineage in the co-culture environment using dual immunocytochemistry. The co-culture cells were stained with cardiac specific marker proteins like actinin and troponin and MSC specific marker protein CD 105 for proving the cardiogenic differentiation of MSCs. Further the morphology of cells was analyzed using SEM.RESULTS: PGS/collagen core/shell fibers, core is PGS polymer having an elastic modulus related to that of cardiac fibers and shell as collagen, providing natural environment for cellular activities like cell adhesion, proliferation and differentiation. SEM micrographs of electrospun fibrous scaffolds revealed porous, beadless, uniform fibers with a fiber diameter in the range of 380 ± 77 nm and 1192 ± 277 nm for collagen fibers and PGS/collagen core/shell fibers respectively. The obtained PGS/collagen core/shell fibrous scaffolds were hydrophilic having a water contact angle of 17.9 ± 4.6° compared to collagen nanofibers which had a contact angle value of 30 ± 3.2°. The PGS/collagen core/shell fibers had mechanical properties comparable to that of native heart muscle with a young's modulus of 4.24 ± 0.7 MPa, while that of collagen nanofibers was comparatively higher around 30.11 ± 1.68 MPa. FTIR spectrum was performed to confirm the functional groups present in the electrospun scaffolds. Amide Ⅰ and amide Ⅱ of collagen were detected at 1638.

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Section: Introductionmentioning

confidence: 99%

Cardiogenic differentiation of mesenchymal stem cells on elastomeric poly (glycerol sebacate)/collagen core/shell fibers

Ravichandran¹

2013

WJC

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“…A high molecular weight, elastomeric PLCL (50:50) was synthesized by Jeong et al for vascular applications (119). This material possessed a T g of À 6.31C and was flexible with elongations exceeding 200% and high recovery after deformation (119).…”

Section: Polyester-based Scaffoldsmentioning

confidence: 99%

“…This material possessed a T g of À 6.31C and was flexible with elongations exceeding 200% and high recovery after deformation (119). Degradation of this material was relatively slow with a mass loss of 1.7% in vitro and 10.7% in vivo at 8 weeks (120).…”

Section: Polyester-based Scaffoldsmentioning

confidence: 99%

Soft Tissue Scaffolds

Guan

Stankus

Wagner

2006

Wiley Encyclopedia of Biomedical Engineering

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The effort to develop soft tissues is one of the most demanding and challenging applications in tissue engineering. Soft tissues such as myocardium, blood vessels, skeletal muscle, adipose tissue, and even cartilage often possess large volumes, have high cell densities, and can be mechanically active. 3‐D scaffolds that match the mechanical properties of the tissue that they are replacing are preferred for soft tissue engineering, because such materials might transmit mechanical forces to the developing tissue during in vitro or in vivo development. A well‐defined biodegradation rate is ideal so that host tissue can replace the scaffold and that stress can be transferred from the support scaffold to the new tissue over an appropriate time period. The scaffolds not only serve as a structural support, but also can play an important role in facilitating cell adhesion, growth, and vascularization throughout the scaffold both during in vitro cell culture and in vivo tissue regeneration. Currently used materials meeting these criteria are diverse and include entirely synthetic materials as well as those that are derived from natural sources, and formats that are continuous on a microscale (hydrogels) and those that have defined microporous architectures. Although natural materials have the inherent advantage of bioactivity, they also possess limitations when employed as soft tissue scaffolds that may be overcome with synthetics. Some limitations associated with natural materials include inducement of an immune response that can lead to rapid degradation as well as poor batch‐to‐batch consistencies. Synthetic polymers permit better control over chemical and physical properties leading to tunability in mechanical strength and degradation rate. Surface properties of synthetic materials can also be more easily modified for improved cell attachment or migration. Some disadvantages of synthetic tissue scaffolds consist of potential toxic degradation products and undesired inflammatory responses. In addition, fabrication methods can process materials into scaffolds of desired porosity, morphologies, and anisotropies. The focus of this article will provide a brief overview of the scaffold preparation, properties, and applications of soft tissue scaffolds.

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“…In the last two decades, numerous efforts have been made to develop scaffolds for vascular tissue engineering by combining material synthesis and scaffold processing techniques. A variety of synthetic materials such as poly(caprolactone) (PCL) [4,5], poly (L-lactide) (PLA) [6], poly (glycolide) (PGA) [7] and naturally occurred materials such as collagen [8,9] and fibrin [10][11][12] can be employed for vascular substitutes and scaffolds for vascular tissue engineering. To engineer materials mimicking the physical structure and biological function of natural blood vessel, a few technologies have been exploited with varying degrees of success [10,11,[13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Physical-chemical Properties and in vitro Biocompatibility Assessment of Spider Silk, Collagen and Polyurethane Nanofiber Scaffolds for Vascular Tissue Engineering

He¹,

Zhang²,

Wang³

et al. 2009

Nano BioMed ENG

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In this study, three kinds of nanofiber scaffolds including spider silks (SS), collagen, and polyurethane (PU) were fabricated by electrospinning technique. Their physical-chemical properties such as surface hydrophilicity, water stability, and porosity were investigated by water contact angle (CA) measurement, stabilization assay and scanning electron microscope (SEM). Results showed that SS scaffolds had stronger hydrophobic surface, superior water-stability and higher porosity than other scaffolds. Furthermore, their in vitro biocompatibility including cell attachment, spreading, and proliferation were evaluated and compared by using porcine aorta endothelium cells (PIECs). The MTT results showed that the cell proliferation on SS nanofibers was significantly higher than that on collagen and PU scaffoldss, the SEM images demonstrated that the PIECs can migrate into SS nanofibers and maintain a spreading shape, and the RT-PCR results also indicated the SS nanofiber scaffolds promote better cell growth and proliferation. Thus, these results strongly suggest the potential application of SS nanofibers as vascular engineering scaffolds. Keywords:

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Manufacture of elastic biodegradable PLCL scaffolds for mechano-active vascular tissue engineering

Cited by 166 publications

References 31 publications

Cardiogenic differentiation of mesenchymal stem cells on elastomeric poly (glycerol sebacate)/collagen core/shell fibers

Cardiogenic differentiation of mesenchymal stem cells on elastomeric poly (glycerol sebacate)/collagen core/shell fibers

Soft Tissue Scaffolds

Physical-chemical Properties and in vitro Biocompatibility Assessment of Spider Silk, Collagen and Polyurethane Nanofiber Scaffolds for Vascular Tissue Engineering

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