Manually Operated Freeze–Clamping Press

Gagnon, Richard; Lehoux, Eric A.; Guderley, Helga

doi:10.1006/abio.1998.2703

Cited by 5 publications

(2 citation statements)

References 12 publications

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“…Each half was quickly frozen in liquid nitrogen (less than 30 s) using a clamping press (Gagnon et al 1998) and stored at -80°C until use for biochemical analysis.…”

Section: Muscle Samplingmentioning

confidence: 99%

Scallops Show That Muscle Metabolic Capacities Reflect Locomotor Style and Morphology

Tremblay¹,

Guderley²

2014

Physiological and Biochemical Zoology

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Although all scallops swim using their adductor muscle to close their valves, scallop species differ considerably in how they use their muscle during escape responses, in parallel with the striking interspecific differences in shell morphology. This provides an excellent opportunity to study links between muscle metabolic capacities and animal performance. We found that the capacity for anaerobic glycolysis and aerobic metabolism, as well as phosphoarginine levels in the phasic adductor muscle, differ with escape response strategy. Phosphoarginine contents were high in species that rely on phasic contractions (Amusium balloti, Placopecten magellanicus, and Pecten fumatus). Arginine kinase activities reflect reliance on rapid initial bursts of phasic contractions. Scallops that maintain their valves in a closed position for prolonged periods (P. fumatus, Mimachlamys asperrima, and Crassadoma gigantea) have high activities of enzymes of anaerobic glycolysis in their phasic adductor muscle. Myosin ATPase activity was lower in the nonswimming scallop, C. gigantea, than in swimming scallops. The different patterns and roles of swimming are reflected in interspecific differences in the biochemical attributes of the phasic adductor muscle. These patterns suggest coevolution of muscle metabolic capacities, patterns of adductor muscle use, and shell morphology in scallops.

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“…Each half was quickly frozen in liquid nitrogen (less than 30 s) using a clamping press (Gagnon et al 1998) and stored at -80°C until use for biochemical analysis.…”

Section: Muscle Samplingmentioning

confidence: 99%

Scallops Show That Muscle Metabolic Capacities Reflect Locomotor Style and Morphology

Tremblay¹,

Guderley²

2014

Physiological and Biochemical Zoology

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show abstract

“…The scallops were returned to a wet-laboratory at Havre St. Pierre and maintained in running seawater (4 to 6°C, 30 psu) for at least 2 d without disturbance before tissue sampling and dissection. At each sampling period, one part of the phasic muscle was rapidly excised and quickly frozen (~1 min) using a freeze-clamping press (Gagnon et al 1998) that had been chilled in liquid nitrogen. We determined the shell height and muscle wet and dry mass (approximately 0.5 g, oven-dried for 48 h at 70°C).…”

Section: Methodsmentioning

confidence: 99%

Binding of glycolytic enzymes in adductor muscle of Iceland scallop Chlamys islandica is altered by reproductive status

Brokordt¹,

Guderley²

2004

Mar. Ecol. Prog. Ser.

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Hydraulic freeze-clamping press fixation system for quick metabolic arrest in medium-sized carcasses

Wong

Fournier

2005

Analytical Biochemistry

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Manually Operated Freeze–Clamping Press

Cited by 5 publications

References 12 publications

Scallops Show That Muscle Metabolic Capacities Reflect Locomotor Style and Morphology

Scallops Show That Muscle Metabolic Capacities Reflect Locomotor Style and Morphology

Binding of glycolytic enzymes in adductor muscle of Iceland scallop Chlamys islandica is altered by reproductive status

Hydraulic freeze-clamping press fixation system for quick metabolic arrest in medium-sized carcasses

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