Manual biología reproductiva y citológica de la papa

Ordoñez, B.; Orrillo, M.; Bonierbale, Merideth

doi:10.4160/9789290604778

2016

DOI: 10.4160/9789290604778

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Manual biología reproductiva y citológica de la papa

B. Ordoñez¹,

M. Orrillo²,

Merideth Bonierbale³

Abstract: El Centro Internacional de la Papa (CIP) es una organización de investigación para el desarrollo especializada en papa, camote y raíces y tubérculos andinos. Su objetivo es brindar soluciones sostenibles basadas en la ciencia a los acuciantes problemas de hambre, pobreza, igualdad de género, cambio climático y preservación de la frágil biodiversidad de nuestro planeta y sus recursos naturales. www.cipotato.orgEl CIP es miembro del CGIAR. El CGIAR es una alianza mundial de investigación agrícola para un futuro … Show more

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Cited by 2 publications

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Testing two chromosome doubling agents for in vitro tetraploid induction on ginger lilies, Hedychium gardnerianum Shepard ex Ker Gawl. and Hedychium coronarium J. Koenig

Mohammadmehdi

Shukurova

Watanabe

2021

In Vitro Cell.Dev.Biol.-Plant

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Polyploidization can be a way to produce new varieties in vegetatively propagated species where options on increasing genetic variability are limited compared with sexual reproduction. While there are hundreds of publications with in vitro methods in somatic doubling, it is cardinal to custom-test for a target species of interest on choosing specific reagents and optimizing conditions. This research was performed to provide a reference process for Zingiberaceae species of which the majority is reproduced with vegetative propagation. Ginger lilies, Hedychium gardnerianum Shepard ex Ker Gawl. and Hedychium coronarium J. Koenig, were employed to optimize chromosome doubling for tetraploid production as they are typically used by vegetative propagation. However, they have the popularity as ornamentals globally due to their horticultural aspects. Tetraploid induction was optimized by in vitro somatic chromosome doubling on H. coronarium and H. gardnerianum through callogenesis. The explant segments of young leaf blades or leaf sheaths were treated with different concentrations of either with colchicine or with oryzalin. The regenerated shoots from callus cultures were transferred to basal MS medium for 2mo to confirm somatic stability. The ploidy was assessed by flow cytometry, measuring the size and density of stomata, counting chromosomes, and chloroplasts in guard cells. The highest percentage of tetraploid regenerated plants was observed with 1250 µM colchicine treatment for Hedychium gardnerianum when compared to the other treatments tested, while no tetraploid plants were obtained from the oryzalin treatment. In H. coronarium, four mixoploid regenerated plants treated with 15 µM oryzalin were confirmed and no shoots were obtained from the colchicine treatment. The results indicated that the in vitro polyploid induction is optimized with the two Hedychium species, which could reference other Zingiberaceae species.

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Testing two chromosome doubling agents for in vitro tetraploid induction on ginger lilies, Hedychium gardnerianum Shepard ex Ker Gawl. and Hedychium coronarium J. Koenig

Mohammadmehdi

Shukurova

Watanabe

2021

In Vitro Cell.Dev.Biol.-Plant

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Genetic and phenotypic diversity of native potatoes (Solanum spp.) from the Central Andes of Peru

Zevallos Arias,

Inga Ortíz,

Marmolejo Gutarra

et al. 2023

Acta Agron.

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The diversity of native potatoes cultivated above 3500 masl in the Pasco region (Central Andes of Peru) has not been fully characterized. It is currently subject to constant genetic erosion caused by biotic and abiotic factors. The research aimed to characterize phenotypically and genotypically 40 native potato landraces representative of 4 Solanum species. Twenty phenotypic descriptors and 10 microsatellites were used for genetic evaluation. Likewise, the ploidy level was evaluated based on the number of chloroplasts in the stomata. The clustering analysis was performed using the Infostat software and the R program with the Adegenet and Polysat libraries. The phenotypic characterization allowed to obtain five groups with a distance coefficient of 9.5. The molecular characterization found seven groups and 58 alleles in total. The average number of alleles per microsatellite was 5.5. 13.2 % of duplicates were identified. The microsatellites STG001, STM1106, ST0032, and STM5127 with an average He of 0.8 and a polymorphism information content (PIC) of 0.5 - 0.8 were the most informative. Finally, the ploidy results were 13 % diploid, 35 % triploid, and 52 % tetraploid. It was evidenced low diversity when using a set of 10 SSR markers, which indicates limited applicability for studying the genetic diversity of local potato landraces. It is necessary to involve a broader range of markers and a more diverse set of genotypes from the Pasco region for further studies.

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Manual biología reproductiva y citológica de la papa

Cited by 2 publications

References 12 publications

Testing two chromosome doubling agents for in vitro tetraploid induction on ginger lilies, Hedychium gardnerianum Shepard ex Ker Gawl. and Hedychium coronarium J. Koenig

Testing two chromosome doubling agents for in vitro tetraploid induction on ginger lilies, Hedychium gardnerianum Shepard ex Ker Gawl. and Hedychium coronarium J. Koenig

Genetic and phenotypic diversity of native potatoes (Solanum spp.) from the Central Andes of Peru

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