Manual acupuncture improves parameters associated with oxidative stress and inflammation in PTZ-induced kindling in mice

Frantz, Alexsandro Luís; Regner, Gabriela Gregory; Pflüger, Pricila; Coelho, Vanessa Rodrigues; Silva, Lucas Lima da; Viau, Cassiana Macagnan; Souza, Marcele Silva De; Silva, Juliana Bondan da; Picada, Jaqueline Nascimento; Saffi, Jenifer; Pereira, Patrı́cia

doi:10.1016/j.neulet.2017.09.044

Cited by 13 publications

(7 citation statements)

References 35 publications

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“…In our study, PTZ kindling increased DNA damage in the hippocampi of mice, as previously demonstrated in another study [47]. Treatment with LCM 20 and 30 mg/kg decreased DNA damage, suggesting neuroprotective effects.…”

Section: Discussionsupporting

confidence: 91%

Lacosamide improves biochemical, genotoxic, and mitochondrial parameters after PTZ‐kindling model in mice

Lazzarotto

Pflüger

Regner

et al. 2020

Fundamemntal Clinical Pharma

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This study evaluated the effect of lacosamide (LCM) on biochemical and mitochondrial parameters after PTZ kindling in mice. Male mice were treated on alternative days for a period of 11 days with LCM (20, 30, or 40 mg/kg), saline, or diazepam (2 mg/kg), before PTZ administration (50 mg/kg). The hippocampi were collected to evaluate free radicals, the activities of superoxide dismutase (SOD), catalase (CAT), and the mitochondrial complexes I‐III, II, and II‐III, as well as Bcl‐2 and cyclo‐oxygenase‐2 (COX‐2) expressions. Hippocampi, blood, and bone marrow were collected for genotoxic and mutagenic evaluations. LCM 40 mg/kg increased latency and decreased percentage of seizures, only on the 3rd day of observation. The dose of 30 mg/kg only showed positive effects on the percentage of seizures on the 2nd day of observation. LCM decreased free radicals and SOD activity and the dose of 40 mg/kg were able to increase CAT activity. LCM 30 and 40 mg/kg improved the enzymatic mitochondrial activity of the complex I‐III and LCM 30 mg/kg improved the activity of the complex II. In the comet assay, the damage induced by PTZ administration was reduced by LCM 20 and 30 mg/kg. The dose of 20 mg/kg increased COX‐2 expression while the highest dose used, 40 mg/kg, was able to reduce this expression when compared to the group treated with LCM 20 mg/kg. Although LCM did not produce the antiepileptogenic effect in vivo, it showed the neuroprotective effect against oxidative stress, bioenergetic dysfunction, and DNA damage induced by the repeated PTZ administration.

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Section: Discussionsupporting

confidence: 91%

Lacosamide improves biochemical, genotoxic, and mitochondrial parameters after PTZ‐kindling model in mice

Lazzarotto

Pflüger

Regner

et al. 2020

Fundamemntal Clinical Pharma

Self Cite

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“…In the pathogenesis of chronic epilepsy, a large number of superoxide anions can be produced, and the endogenous antioxidant enzymes SOD, GSH, GSR and cat are rapidly consumed, resulting in the production of a large number of toxic lipid peroxide increased and induced the oxidative stress. In addition, in PTZ-induced kindling in mice, it was found that reactive oxygen species was activated, and its production agrees with decrease in antioxidant related enzymes (Frantz et al 2017;Chindo et al 2021). In this study, we found that treated with GEAT decoction reduced MDA levels in PTZ-kindled mouse hippocampus, while increased CAT and SOD activities, as well as increased GSH levels when compared with that of PTZ-kindled mice.…”

Section: Discussionsupporting

confidence: 65%

Evaluation of the Anticonvulsant Activities of Gastrodia Elata Bl.- Acorus Tatarinowii Decoction on Experimentally Induced Seizures in Mice

Yang

Yuan

et al. 2022

Preprint

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Epilepsy is a serious public health problem in the world. At present, the effect of drug treatment of epilepsy is are not satisfactory. Medicinal plants as pharmaceuticals and for healthcare treatments in the management of epilepsy in China for many centuries. Especially, Gastrodia elata Bl.-Acorus tatarinowii, as a classic and important herb pairs in folk medicine, has been used in folk medicine to control seizures. However, the animal experiment data of its anticonvulsant effect is limited in the literature. The objective of this study was to mainly analyze the anticonvulsant activity of Gastrodia elata-Acorus tatarinowii (GEAT) decoction in maximal electroshock (MES), pentylenetetrazole (PTZ) and trimercaptopropionic acid (3-MP) induced seizures in mice, providing scientific basis for the treatment of convulsive disorders in traditional medicine. In addition, the improvement effect were examined on seizure severity, anxiety, cognitive dysfunction, inflammation and oxidative stress in PTZ kindled mice. The results showed that GEAT decoction dose-dependently protected mice against MES, 3-MP and PTZ induced acute seizures. Meanwhile, GEAT decoction ameliorated seizure severity, decreased the accumulation of inflammatory mediators TNF-α, IL-1β, and IL-6, mitigated oxidative stress, as well as alleviated anxious-like behavior and cognitive deficits in PTZ-kindled mice. Our data evidenced that the anticonvulsant properties attributed to GEAT decoction as adjunctive therapy for epileptic patients in folk medicine.

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“… 77 In the pentylenetetrazole (PTZ)-induced epileptic model, MA at GV20 was shown to reduce ROS production, increase SOD and catalase (CAT) activity, inhibit expression of pro-inflammatory factor TNF-α, produce anti-epileptic signal, inhibit abnormal discharge of damaged neurons, and finally reduce the degree of epileptic seizure. 78 …”

Section: Resultsmentioning

confidence: 99%

“…77 In the pentylenetetrazole (PTZ)induced epileptic model, MA at GV20 was shown to reduce ROS production, increase SOD and catalase (CAT) activity, inhibit expression of pro-inflammatory factor TNF-α, produce anti-epileptic signal, inhibit abnormal discharge of damaged neurons, and finally reduce the degree of epileptic seizure. 78 When oxygen and ATP energy, required for cell metabolism, are insufficient and the excitotoxicity of glutamate increases, over-activation of the N-methyl-D-aspartate receptor (NMDAR) and, eventually, cell death may result. Acupuncture modulates this process by inhibiting the inflammatory response.…”

Section: Glial Cellsmentioning

confidence: 99%

The Anti-Inflammatory Actions and Mechanisms of Acupuncture from Acupoint to Target Organs via Neuro-Immune Regulation

Li¹,

Guo²,

Gong³

et al. 2021

JIR

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Inflammation plays a significant role in the occurrence and development of multiple diseases. This study comprehensively reviews and presents literature from the last five years, showing that acupuncture indeed exerts strong anti-inflammatory effects in multiple biological systems, namely, the immune, digestive, respiratory, nervous, locomotory, circulatory, endocrine, and genitourinary systems. It is well known that localized acupuncture-mediated anti-inflammatory effects involve the regulation of multiple populations and functions of immune cells, including macrophages, granulocytes, mast cells, and T cells. In acupuncture stimulation, macrophages transform from the M1 to the M2 phenotype and the negative TLR4 regulator PPARγ is activated to inhibit the intracellular TLR/MyD88 and NOD signaling pathways. The downstream IκBα/NF-κB and P38 MAPK pathways are subsequently inhibited by acupuncture, followed by suppressed production of inflammasome and proinflammatory mediators. Acupuncture also modulates the balance of helper T cell populations. Furthermore, it inhibits oxidative stress by enhancing SOD activity via the Nrf2/ HO-1 pathway and eliminates the generation of oxygen free radicals, thereby preventing inflammatory cell infiltration. The anti-inflammatory effects of acupuncture on different biological systems are also specific to individual organ microenvironments. As part of its anti-inflammatory action, acupuncture deforms connective tissue and upregulates the secretion of various molecules in acupoints, further activating the NF-κB, MAPK, and ERK pathways in mast cells, fibroblasts, keratinocytes, and monocytes/macrophages. The somatic afferents present in acupuncture-activated acupoints also convey sensory signals to the spinal cord, brainstem, and hypothalamic neurons. Upon information integration in the brain, acupuncture further stimulates multiple neuro-immune pathways, including the cholinergic anti-inflammatory, vagus-adrenal medulla-dopamine, and sympathetic pathways, as well as the hypothalamus-pituitary-adrenal axis, ultimately acting immune cells via the release of crucial neurotransmitters and hormones. This review provides a scientific and reliable basis and viewpoints for the clinical application of acupuncture in various inflammatory conditions.

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Manual acupuncture improves parameters associated with oxidative stress and inflammation in PTZ-induced kindling in mice

Cited by 13 publications

References 35 publications

Lacosamide improves biochemical, genotoxic, and mitochondrial parameters after PTZ‐kindling model in mice

Lacosamide improves biochemical, genotoxic, and mitochondrial parameters after PTZ‐kindling model in mice

Evaluation of the Anticonvulsant Activities of Gastrodia Elata Bl.- Acorus Tatarinowii Decoction on Experimentally Induced Seizures in Mice

The Anti-Inflammatory Actions and Mechanisms of Acupuncture from Acupoint to Target Organs via Neuro-Immune Regulation

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