Mantle cell lymphoma harboring Burkitt’s-like translocations presents differential expression of aurora kinase genes compared with others 8q abnormalities

Abstract

We compared the levels of AURKA and AURKB in 24 (mantle cell lymphoma) MCL patients harboring 8q abnormalities and its relationship with MYCC gene status. Two distinct subgroups were observed, in terms of MYCC expression. Except for the patients with Burkitt's-like translocation, none of the patients harboring 8q abnormalities, including balanced translocations or duplications of MYCC band, identified both by G-banding and SKY, showed differential expression levels of MYCC. These previous findings also reflected in the differential expression of AURKA and AURKB genes. We found that AURKA and AURKB mRNA were expressed at significantly higher levels in MCL patients harboring Burkitt's-like translocation, when compared to patients with 8q rearrangements. The high expression of aurora kinase genes is reported to be associated with some parameters of clinical oncologic aggressiveness, such as high histological grade, invasion and increased rates of metastasis in several types of cancers. It is possible that in MCL patients expressing abnormal levels of MYCC together with a high expression of AURKA might offer some resistant to the conventional therapy purposes. Thus, aurora kinase inhibitors may also be considered for this specific subgroup on MCL, whose aggressive clinical course resembles high-grade lymphoma.

Three-dimensional nuclear telomere architecture and differential expression of aurora kinase genes in chronic myeloid leukemia to measure cell transformation

“…RNA was isolated from CML samples using TRIzol reagent (ThermoFisher, USA) according previously described [ 30 ]. Complementary DNA (cDNA) was synthesized from ~ 1 μg of total RNA using a High-Capacity cDNA reverse transcription Kit (Applied BioSystems, Foster City, CA, USA), following the manufacturer's instructions.…”

“…On comparative analysis of CML samples and healthy donors, AURKA and AURKB gene expression was calculated as a relative quantification to the GAPDH housekeeping gene. The gene expression AURKA and AURKB from CML samples was calculated as relative quantification to normal controls (ΔΔCt = ΔCt patient – ΔCt healthy donors+ ) and expressed as 2 −ΔΔCt , according previously described [ 30 ]. All primers were standardized by conventional semi-quantitative PCR analysis before proceeding to the real-time quantitative analysis.…”

Three-dimensional nuclear telomere architecture and differential expression of aurora kinase genes in chronic myeloid leukemia to measure cell transformation

“…RNA was isolated from CML samples using TRIzol reagent (ThermoFisher, USA) according previously described [30]. Complementary DNA (cDNA) was synthesized from ~ 1µg of total RNA using a High-Capacity cDNA reverse transcription Kit (Applied BioSystems, Foster City, CA, USA), following the manufacturer's instructions.…”

“…On comparative analysis of CML samples and healthy donors, AURKA and AURKB gene expression was calculated as a relative quanti cation to the GAPDH housekeeping gene. The gene expression AURKA and AURKB from CML samples was calculated as relative quanti cation to normal controls (ΔΔCt = ΔCt patient -ΔCt healthy donors+ ) and expressed as 2 −ΔΔCt , according previously described [30]. All primers were standardized by conventional semi-quantitative PCR analysis before proceeding to the real-time quantitative analysis.…”