Mannosylerythritol lipid, a yeast extracellular glycolipid, shows high binding affinity towards human immunoglobulin G

Im, Jae Hong; Nakane, Takashi; Yanagishita, Hiroshi; Ikegami, Tsutomu; Kitamoto, Daï

doi:10.1186/1472-6750-1-5

Cited by 29 publications

(14 citation statements)

References 37 publications

(47 reference statements)

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“…PuriWcation of glycoprotein MEL-A, -B and -C exhibit high binding aYnity to human immunoglobulins [15,16,39,43]. They also show binding aYnity towards Ig M and other glycoproteins.…”

Section: Mel Induces Cell Diverentiation and Apoptosismentioning

confidence: 95%

“…They can be used in the treatment of schizophrenia or diseases caused by dopamine metabolic dysfunction [67,72] and microbial infections [37]. MELs are used in the puriWcation of lectins (Immunoglobulin G) [9,15,16,21,39] and in the preparation of ice-slurry as an antiagglomeration agent [41,42]. Self-assembling properties [17-20, 39, 43, 70] of MELs are of recent interest because this behavior could be leveraged in gene transfection and drug delivery [23,24,42,43,65,66].…”

Section: Mel: Why?mentioning

confidence: 99%

See 1 more Smart Citation

Mannosylerythritol lipids: a review

Arutchelvi¹,

Bhaduri²,

Uppara³

et al. 2008

J Ind Microbiol Biotechnol

160

102

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Mannosylerythritol lipids (MELs) are surface active compounds that belong to the glycolipid class of biosurfactants (BSs). MELs are produced by Pseudozyma sp. as a major component while Ustilago sp. produces them as a minor component. Although MELs have been known for over five decades, they recently regained attention due to their environmental compatibility, mild production conditions, structural diversity, self-assembling properties and versatile biochemical functions. In this review, the MEL producing microorganisms, the production conditions, their applications, their diverse structures and self-assembling properties are discussed. The biosynthetic pathways and the regulatory mechanisms involved in the production of MEL are also explained here.

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“…PuriWcation of glycoprotein MEL-A, -B and -C exhibit high binding aYnity to human immunoglobulins [15,16,39,43]. They also show binding aYnity towards Ig M and other glycoproteins.…”

Section: Mel Induces Cell Diverentiation and Apoptosismentioning

confidence: 95%

Section: Mel: Why?mentioning

confidence: 99%

Mannosylerythritol lipids: a review

Arutchelvi¹,

Bhaduri²,

Uppara³

et al. 2008

J Ind Microbiol Biotechnol

160

102

View full text Add to dashboard Cite

show abstract

“…These MELs exhibit not only excellent interfacial properties [12], but also remarkable differentiation‐inducing activities against human leukaemia cells [13,14], rat pheochromocytoma [15] and mouse melanoma cells [16,17]. MELs also show high binding affinity towards different immunoglobulins and lectins [18–23]. In addition, MEL‐A, the major component of MELs, dramatically increases the efficiency of gene transfection mediated by cationic liposomes [24–27].…”

Section: Melsmentioning

confidence: 99%

“…Previously Im et al [21] reported that MEL‐A shows high binding affinity towards human IgG. The binding behaviour was further investigated by using surface plasmon resonance and the measurement of surface pressure (π)–area ( A ) isotherms [23].…”

Section: Binding Affinity Towards Immunoglobulins and Lectins Of Melmentioning

confidence: 99%

Production of glycolipid biosurfactants by basidiomycetous yeasts

Morita

Fukuoka

Imura

et al. 2009

Biotech and App Biochem

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BSs (biosurfactants) produced by various micro-organisms show unique properties (e.g. mild production conditions, lower toxicity, higher biodegradability and environmental compatibility) compared with chemically synthesized surfactants. The numerous advantages of BSs have prompted applications not only in the food, cosmetic and pharmaceutical industries but also in environmental protection and energy-saving technology. Among BSs, glycolipid types are the most promising, owing to their high productivity from renewable resources and versatile biochemical properties. MELs (mannosylerythritol lipids), which are glycolipid BSs abundantly produced by basidiomycetous yeasts such as strains of Pseudozyma, exhibit not only excellent interfacial properties, but also remarkable differentiation-inducing activities against human leukaemia cells. MELs also show high binding affinity towards different immunoglobulins and lectins. Recently, a cationic liposome bearing MEL has been demonstrated to increase dramatically the efficiency of gene transfection into mammalian cells. These features of BSs should broaden their application in new advanced technologies. In the present review the current status of research and development on glycolipid BSs, especially their production by Pseudozyma yeasts, is described.

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“…1 Several chromatographic techniques including high performance, size exclusion, ion-exchange, hydrophobic interaction, hydroxyapatite, and thiophilic have been used for the purification of immunoglobulins. [2][3][4][5] However, immunoglobulins are often purified by protein A affinity chromatography due to its high selectivity.…”

Section: Introductionmentioning

confidence: 99%

Antibody purification from human plasma by metal‐chelated affinity membranes

Yavuz

Bereli

Armutçu

et al. 2011

J of Applied Polymer Sci

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The aim of this study is to investigate in detail the feasibility of poly(2-hydroxyethyl methacrylate-N-methacryloyl-(L)-histidine methyl ester), PHEMAH membranes for purification of immunoglobulin G (IgG) from human plasma. PHEMAH membranes were prepared by photo-polymerization technique. Then, Zn 2þ , Ni 2þ , Co 2þ , and Cu 2þ ions were chelated directly on the PHEMAH membranes. Elemental analysis assay was performed to determine the nitrogen content and polymerized MAH was calculated as 168.5 lmol/g. The nonspecific IgG adsorption onto the plain PHEMA membranes was negligible (about 0.25 mg/mL). A remarkable increase in the IgG adsorption capacities were achieved from human plasma with PHEMAH membranes (up to 68.4 mg/mL).Further increase was observed with the metal-chelated PHEMAH membranes (up to 118 mg/mL). The metal-chelate affinity membranes allowed the one-step separation of IgG from human plasma. The binding range of metal ions for surface histidines from human plasma followed the order: Cu 2þ > Ni 2þ > Zn 2þ > Co 2þ . Adsorbed IgG was eluted using 250 mM EDTA with a purity of 94.1%. IgG molecules could be repeatedly adsorbed and eluted with the metal-chelated PHEMAH membranes without noticeable loss in their IgG adsorption capacity.

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Mannosylerythritol lipid, a yeast extracellular glycolipid, shows high binding affinity towards human immunoglobulin G

Cited by 29 publications

References 37 publications

Mannosylerythritol lipids: a review

Mannosylerythritol lipids: a review

Production of glycolipid biosurfactants by basidiomycetous yeasts

Antibody purification from human plasma by metal‐chelated affinity membranes

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