Mannosomes: a molluscan intracellular tubular membrane system related to heavy metal stress?

Knigge, Thomas; Mann, Neelam; Parveen, Zahida; Perry, Campbell; Gernhöfer, Maike; Triebskorn, Rita; Köhler, Heinz-Rüdiger; Connock, Martin

doi:10.1016/s1532-0456(02)00013-3

Cited by 6 publications

(4 citation statements)

References 20 publications

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“…Cd 2+ exposure in these animals also induces cellular alterations and programmed cell death Chabicovsky et al 2004;Zaldibar et al 2008). In contrast to the MT defense strategy, cellular alterations attributable to metal intoxication are more difficult to interpret (Dallinger et al 2001a;Knigge et al 2002), and the interplay between MT detoxification and cellular responses is not yet completely understood. Among the several cell-morphological changes seen upon metal exposure, some can be interpreted as an adaptive response aimed at enhancing cellular defence capacity (Dallinger et al 2005), whereas others can clearly be identified as pathological alterations Manzl et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Cadmium stress stimulates tissue turnover in Helix pomatia: increasing cell proliferation from metal tolerance to exhaustion in molluscan midgut gland

et al. 2010

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In terrestrial pulmonate snails, cadmium (Cd) uptake leads to the induction of a Cd-specific metallothionein isoform (Cd-MT) that protects against adverse interactions of this toxic metal ion. Increasing concentrations of Cd cause increased individual mortality possibly linked to pathological alterations in the snail midgut gland. Histological, immuno-histochemical, and electron-microscopic methods in combination with tissue metal analyses and quantification of MT induction parameters were applied to the midgut gland of Cd-exposed Roman snails (Helix pomatia). Conspicuous concentration-dependent alterations occurred in this organ, including the metal-induced increase of Cd-MT concentration and manifestation of Cd-MT mRNA precipitations in all midgut gland cell types. The most evident alteration was an increase of cellular turnover reflected by enhanced cell proliferation. Intensified vesiculation of endoplasmic reticulum was noted in basophilic cells and an increasing formation of lipofuscin granules in excretory cells. At the highest Cd concentrations, mitochondrial membranes were disrupted in basophilic cells, and lipofuscin granules were released from excretory cells into the midgut gland tubular system. Some of these alterations (e.g., increased cell proliferation rate, vesiculation of endoplasmic reticulum) detected at low Cd concentrations were interpreted as adaptive response processes enhancing the tolerance of exposed individuals to metal stress. Cellular alterations at higher Cd concentrations (e.g., mitochondrial structural damage) clearly represented ongoing irreversible cellular disruption. Combined evaluation of cellular biomarkers and MT saturation levels indicated that the transition from stress resistance to depletion of resistance capacity occurred above a threshold of 0.8 micromol Cd/g dry weight in the midgut gland of H. pomatia. At these Cd concentrations, Cd-MT was saturated with Cd(2+) ions, whereas at the cellular level, structural alterations turned into pathological deterioration.

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Section: Introductionmentioning

confidence: 99%

Cadmium stress stimulates tissue turnover in Helix pomatia: increasing cell proliferation from metal tolerance to exhaustion in molluscan midgut gland

et al. 2010

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“…depilans bundles of these tubules were found inside rough endoplasmic reticulum cisternae [ 14 ]. Thus, taking into account both biochemical and ultrastructural data, mannitol oxidase carrying structures can be considered a specialized component of the endoplasmic reticulum, named mannosome [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

Mannitol oxidase and polyol dehydrogenases in the digestive gland of gastropods: Correlations with phylogeny and diet

et al. 2018

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Mannitol oxidase and polyol dehydrogenases are enzymes that convert polyalcohols into sugars. Mannitol oxidase was previously investigated in terrestrial snails and slugs, being also present in a few aquatic gastropods. However, the overall distribution of this enzyme in the Gastropoda was not known. Polyol dehydrogenases are also poorly studied in gastropods and other mollusks. In this study, polyalcohol oxidase and dehydrogenase activities were assayed in the digestive gland of 26 species of gastropods, representing the clades Patellogastropoda, Neritimorpha, Vetigastropoda, Caenogastropoda and Heterobranchia. Marine, freshwater and terrestrial species, including herbivores and carnivores were analyzed. Ultrastructural observations were undertake in species possessing mannitol oxidase, in order to investigate the correlation between this enzyme and the presence of tubular structures known to be associated with it. Mannitol oxidase activity was detected in the digestive gland of herbivores from the clades Caenogastropoda and Heterobranchia, but not in any carnivores or in herbivores from the clades Patellogastropoda, Neritimorpha and Vetigastropoda. In most of the species used in this study, dehydrogenase activities were detected using both D-mannitol and D-sorbitol as substrates. Nevertheless, in some carnivores these activities were not detected with both polyalcohols. Ultrastructural observations revealed tubular structures in digestive gland cells of some species having mannitol oxidase activity, but they were not observed in others. Based on our results, we suggest that mannitol oxidase first occurred in a herbivorous or omnivorous ancestor of Apogastropoda, the clade formed by caenogastropods and heterobranchs, being subsequently lost in those species that shifted towards a carnivorous diet.

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“…Potentially, combinations of heavy metals can induce such distinctive patterns of Hsp expression in soil nematodes that these patterns can become diagnostic fingerprints for specific toxicants in soil [62]. Additional examples of Hsp70 expression in environmental monitoring concern rotifers [63], fish [59,[64][65][66][67], mollusks [39,40,[68][69][70][71][72], algae [73], and earthworm [57].…”

Section: Hsp70 In Environmental Monitoringmentioning

confidence: 99%

Heat shock response: hsp70 in environmental monitoring

Mukhopadhyay

Nazir

Saxena

et al. 2003

J Biochem & Molecular Tox

145

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Heat shock proteins (Hsps) are a ubiquitous feature of cells in which these proteins cope with stress-induced denaturation of other proteins. Among the different families of Hsps, the 70 kDa family (hsp70) is the most highly conserved and has been most extensively studied. Apart from their primary role in cellular defense under stress condition, a number of studies in recent years have shown the immense potential of hsp70 in pollution monitoring using even transgenic approach both in vivo and in vitro. This article reviews the recent developments in the widespread application of hsp70 in environmental risk assessment. C

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Mannosomes: a molluscan intracellular tubular membrane system related to heavy metal stress?

Cited by 6 publications

References 20 publications

Cadmium stress stimulates tissue turnover in Helix pomatia: increasing cell proliferation from metal tolerance to exhaustion in molluscan midgut gland

Cadmium stress stimulates tissue turnover in Helix pomatia: increasing cell proliferation from metal tolerance to exhaustion in molluscan midgut gland

Mannitol oxidase and polyol dehydrogenases in the digestive gland of gastropods: Correlations with phylogeny and diet

Heat shock response: hsp70 in environmental monitoring

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