Mannose-Resistant Haemagglutination and P Antigen Recognition Are Characteristic of Escherichia Coli Causing Primary Pyelonephritis

Novel cell‐binding activity specific for N‐acetyl‐D‐glucosamine in an Escherichia coli strain

“…We therefore tested 65 non-haemagglutinating strains of E. coli using endo-,&galactosidase treated Pi erythrocytes to reveal N-acetyl-D-glucosamine recognition. In addition 38 strains known to contain blood-group P-specific adhesion activity [7][8][9] were tested with endow-galactosid~e treated p erythrocytes and 3 strains known to contain bloodgroup M-binding specificity [lo] were tested with enzyme-treated NN erythrocytes to avoid haemagglutinations due to these binding specificities.…”

“…The P-fimbriated strains were mostly pyelonephritogenic and represented all the common serotypes of E. coli strains associated with human pyelonephritis. Thus, although strain IH 11165 was isolated from a l-year-old girl with acute pyelonephritis [9,10], it seems that the novel binding specificity is rare at least among human pyelonephritogenic strains.…”

“…Three E. coli strains showing marmose-resistant blood-group M-specific haemagglutination were studied: IH 11165 (serotype 02) was isolated from a patient with acute pyelonephritis [IO], IH 3082 (08H19) from a patient with newborn meningitis, and KS 300 was kindly donated by Dr G. Kglllenius (National Bacteriological Laboratory, Stockholm) and was not typable with the antisera available [9]. In addition, 63 strains lacking D-mannose-resistant haemagglutination activity of human OPI or Op erythrocytes, and 38 strains showing only Pspecific agglutination, were tested.…”

“…One of the Pfimbriated strains was not typable with the sera available. For haemagglutination tests the bacteria were grown on CFA-agar plates as in [9].…”

Correlation between electrophoretic types B1and B2of carboxylesterase B and host-dependent factors inEscherichia colisepticaemia

“…some 0 and K antigens (Orskov et al 1977; Orskov & Orskov, 1985), resistance to serum bactericidal activity (Taylor, 1983;Cross et al 1986), virulence to mice and to chicken embryo (Minshew et al 1978; Van den Bosch, De Graaff & MacLaren, 1979; Bosch, Emody & Ketyi, 1982), aerobactin production (Carbonetti et al 1986), a-haemolysin production (Van den Bosch et al 1981;Welch et al 1981;Cavalieri, Bohach & Snyder, 1984) and mannose resistant haemagglutinin (Evans et al 1980;Hagberg et al 1981;Vaisanen et al 1981). However, these infections not only depend on the intrinsic properties of the strains but also on various hostdependent factors, such as susceptibility linked to an underlying disease (Singer, * Author for reprints.…”