Mannose receptor mediated phagocytosis of bacteria in macrophages of blunt snout bream (Megalobrama amblycephala) in a Ca2+-dependent manner

Zhao, Xiaoheng; Liu, Lichun; Hegazy, Abeer M.; Wang, Hong; Li, Jie; Zheng, Feifei; Zhou, Yang; Wang, Weimin; Li, Jun; Liu, Xiaoling; Lin, Li

doi:10.1016/j.fsi.2015.01.002

Cited by 30 publications

(7 citation statements)

References 23 publications

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“…The binding curve showed that the binding activity of COS6 and MR CTLD4-8 is almost the same as mannose, which was previously considered the best ligand. The binding of Ca 2+ between MR and ligand in mammals is essential, and we have previously reported that MaMR CTLD4-8-mediated phagocytosis of bacteria is also Ca 2+ -dependent ( 40 ). We think that MaMR CTLD4-8 also requires the participation of Ca 2+ in the recognition and binding of COS6.…”

Section: Discussionmentioning

confidence: 99%

Mannose Receptor Mediates the Activation of Chitooligosaccharides on Blunt Snout Bream (Megalobrama amblycephala) Macrophages

Ouyang

Wang

et al. 2021

Front. Immunol.

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Chitooligosaccharide (COS) is an important immune enhancer and has been proven to have a variety of biological activities. Our previous research has established an M1 polarization mode by COS in blunt snout bream (Megalobrama amblycephala) macrophages, but the mechanism of COS activation of blunt snout bream macrophages remains unclear. In this study, we further explored the internalization mechanism and signal transduction pathway of chitooligosaccharide hexamer (COS6) in blunt snout bream macrophages. The results showed that mannose receptor C-type lectin-like domain 4-8 of M. amblycephala (MaMR CTLD4-8) could recognize and bind to COS6 and mediate COS6 into macrophages by both clathrin-dependent and caveolin-dependent pathways. In the inflammatory response of macrophages activated by COS6, the gene expression of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and nitric oxide synthase 2 (NOS2) was significantly inhibited after MaMR CTLD4-8-specific antibody blockade. However, even if it was blocked, the expression of these inflammation-related genes was still relatively upregulated, which suggested that there are other receptors involved in immune regulation. Further studies indicated that MaMR CTLD4-8 and Toll-like receptor 4 (TLR4) cooperated to regulate the pro-inflammatory response of macrophages caused by COS6. Taken together, these results revealed that mannose receptor (MR) CTLD4-8 is indispensable in the process of recognition, binding, internalization, and immunoregulation of COS in macrophages of blunt snout bream.

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Section: Discussionmentioning

confidence: 99%

Mannose Receptor Mediates the Activation of Chitooligosaccharides on Blunt Snout Bream (Megalobrama amblycephala) Macrophages

Ouyang

Wang

et al. 2021

Front. Immunol.

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“…In fish, multiple CTLs are identified and their roles in immunity have been characterized. Some of these CTLs are located on the cell membrane: for instance, mannose receptor on macrophages of Megalobrama amblycephala mediates phagocytosis of bacteria in a Ca 2+ ‐dependent manner (Liu et al., 2014; Zhao et al., 2015); a hepatic lectin of zebrafish participates in immune defence by binding to a wide range of bacteria (Yang et al., 2018); and a C‐type lectin domain family 4 member (CTL4) from black rockfish promotes bactericidal activity but facilitates virus escape (Xue, Guang‐Hua, Yan‐Li, Min, & Yong‐Hua, 2018). The functions of secreted CTLs in fish immunity are more frequently reported: for instance, in grass carp Ctenopharyngodon Idella and Nile tilapia Oreochromis niloticus , mannose‐binding lectins activate the complement system, possess bacterial agglutinating activity and defend against the bacterial pathogen (Liu & Dang, 2020); in spotted knifejaw Oplegnathus punctatus , a CTL exhibits antibacterial and anti‐inflammatory activities (Liu et al., 2019); and in O. niloticus , a CTL (CL11X1‐like) possesses opsonization ability against bacterial pathogens (Mu et al., 2018).…”

Section: Introductionmentioning

confidence: 99%

A C‐type lectin with antibacterial activity in weather loach, Misgurnus anguillicaudatus

Zhang

Yang

Zhang³

et al. 2020

Journal of Fish Diseases

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C‐type lectins are carbohydrate‐binding proteins that play important roles in immunity by serving as pattern recognition receptors. In the present study, a novel nattectin‐like C‐type lectin was obtained from the weather loach, Misgurnus anguillicaudatus, designated as MaCTL. MaCTL encodes a peptide with 165 amino acids, with a signal peptide and a single C‐type lectin domain (CTLD), containing a galactose‐specific QPD motif and a conserved Ca2+‐binding site. Transcripts of MaCTL were significantly upregulated after immune challenge with its pathogen A. hydrophila. In vitro assays with recombinant MaCTL protein revealed that it exhibited hemagglutinating and bacterial agglutinating activities, in a Ca2+‐dependent manner. MaCTL was found to bind to a wide range of bacteria, as well as bind to bacterial polysaccharides LPS and PGN. Moreover, MaCTL displayed antimicrobial activity by inhibiting the growth of bacteria. These results collectively suggest that MaCTL is involved in the antibacterial defence of weather loach.

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“…We have isolated a strain of A. sobria which could cause the disease of zebra fish in our lab. We have focused our studies on the functions of teleost MRs [24,25,26,27]. In the present report, we cloned and characterized the functions of zebra fish MR using the established zebra fish- A. sobria infection model.…”

Section: Introductionmentioning

confidence: 99%

Molecular Cloning and Functional Characterization of Mannose Receptor in Zebra Fish (Danio rerio) during Infection with Aeromonas sobria

Zheng

Asim

Lan

et al. 2015

IJMS

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Mannose receptor (MR) is a member of pattern-recognition receptors (PRRs), which plays a significant role in immunity responses. Much work on MR has been done in mammals and birds while little in fish. In this report, a MR gene (designated as zfMR) was cloned from zebra fish (Danio rerio), which is an attractive model for the studies of animal diseases. The full-length cDNA of zfMR contains 6248 bp encoding a putative protein of 1428 amino acids. The predicted amino acid sequences showed that zfMR contained a cysteine-rich domain, a single fibronectin type II (FN II) domain, eight C-type lectin-like domains (CTLDs), a transmembrane domain and a short C-terminal cytoplasmic domain, sharing highly conserved structures with MRs from the other species. The MR mRNA could be detected in all examined tissues with highest level in kidney. The temporal expression patterns of MR, IL-1β and TNF-α mRNAs were analyzed in the liver, spleen, kidney and intestine post of infection with Aeromonas sobria. By immunohistochemistry assay, slight enhancement of MR protein was also observed in the spleen and intestine of the infected zebra fish. The established zebra fish-A. sobria infection model will be valuable for elucidating the role of MR in fish immune responses to infection.

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Mannose receptor mediated phagocytosis of bacteria in macrophages of blunt snout bream (Megalobrama amblycephala) in a Ca2+-dependent manner

Cited by 30 publications

References 23 publications

Mannose Receptor Mediates the Activation of Chitooligosaccharides on Blunt Snout Bream (Megalobrama amblycephala) Macrophages

Mannose Receptor Mediates the Activation of Chitooligosaccharides on Blunt Snout Bream (Megalobrama amblycephala) Macrophages

A C‐type lectin with antibacterial activity in weather loach, Misgurnus anguillicaudatus

Molecular Cloning and Functional Characterization of Mannose Receptor in Zebra Fish (Danio rerio) during Infection with Aeromonas sobria

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