Mannose receptor contribution to <i>Candida albicans</i> phagocytosis by murine E-clone J774 macrophages

Porcaro, Isabelle; Vidal, Michel; Jouvert, S.; Stahl, Philip D.; Gadrey, Jean

doi:10.1189/jlb.1202608

Cited by 46 publications

(39 citation statements)

References 70 publications

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“…Purified ␤-1,2 mannosides have been shown to elicit TNF-␣ (20) and eicosanoid (21) production. However, in contrast to other fungal mannosides, which react with C-lectins, such as the mannose receptor (22) or DC-SIGN/SIGNR1 (12), ␤-1,2 mannosides bind to a host 32-kDa protein, identified as the S-lectin galectin-3 (23). In this study, we investigated the physiological role of galectin-3 in the immune response to C. albicans.…”

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confidence: 99%

Specific Recognition of Candida albicans by Macrophages Requires Galectin-3 to Discriminate Saccharomyces cerevisiae and Needs Association with TLR2 for Signaling

Jouault

Behi

Martínez‐Esparza

et al. 2006

The Journal of Immunology

212

179

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Stimulation of cells of the macrophage lineage is a crucial step in the sensing of yeasts by the immune system. Glycans present in both Candida albicans and Saccharomyces cerevisiae cell walls have been shown to act as ligands for different receptors leading to different stimulating pathways, some of which need receptor co-involvement. However, among these ligand-receptor couples, none has been shown to discriminate the pathogenic yeast C. albicans. We explored the role of galectin-3, which binds C. albicans β-1,2 mannosides. These glycans are specifically and prominently expressed at the surface of C. albicans but not on S. cerevisiae. Using a mouse cell line and galectin-3-deleted cells from knockout mice, we demonstrated a specific enhancement of the cellular response to C. albicans compared with S. cerevisiae, which depended on galectin-3 expression. However, galectin-3 was not required for recognition and endocytosis of yeasts. In contrast, using PMA-induced differentiated THP-1, we observed that the presence of TLR2 was required for efficient uptake and endocytosis of both C. albicans and S. cerevisiae. TLR2 and galectin-3, which are expressed at the level of phagosomes containing C. albicans, were shown to be associated in differentiated macrophages after incubation with this sole species. These data suggest that macrophages differently sense C. albicans and S. cerevisiae through a mechanism involving TLR2 and galectin-3, which probably associate for binding of ligands expressing β-1,2 mannosides specific to the C. albicans cell wall surface.

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Specific Recognition of Candida albicans by Macrophages Requires Galectin-3 to Discriminate Saccharomyces cerevisiae and Needs Association with TLR2 for Signaling

Jouault

Behi

Martínez‐Esparza

et al. 2006

The Journal of Immunology

212

179

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“…29 Other CLRs, MR and DC-SIGN recognize N-linked mannan and specifically mediate C. albicans binding and internalization via phagocytes. [30][31][32] Although MR lacks signaling motifs, it performs endocytosis, mediates internalization of their ligands and also contributes to antigen presentation. 33 Galectin-3, an S-type lectin receptor, recognizes b -(1-2) oligomannan and efficiently helps defend against disseminated C. albicans in a mouse model.…”

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confidence: 99%

Innate immune cell response uponCandida albicansinfection

Qin

Zhang

et al. 2016

Virulence

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Candida albicans is a polymorphic fungus which is the predominant cause of superficial and deep tissue fungal infections. This microorganism has developed efficient strategies to invade the host and evade host defense systems. However, the host immune system will be prepared for defense against the microbe by recognition of receptors, activation of signal transduction pathways and cooperation of immune cells. As a consequence, C. albicans could either be eliminated by immune cells rapidly or disseminate hematogenously, leading to life-threatening systemic infections. The interplay between Candida albicans and the host is complex, requiring recognition of the invaded pathogens, activation of intricate pathways and collaboration of various immune cells. In this review, we will focus on the effects of innate immunity that emphasize the first line protection of host defense against invaded C. albicans including the basis of receptor-mediated recognition and the mechanisms of cell-mediated immunity.

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“…This receptor recognizes glycosylated molecules with terminal mannose, fucose, or N-acetylglucosamine moieties and internalizes soluble and particulate ligands through the endocytic and phagocytic pathways. Its capacity for ligand recognition makes this receptor suitable to phagocytose Candida albicans, Leishmania donovani, and Pneumocytis carinni, among other microorganisms (2)(3)(4). The MR is the prototypic element of a homonymous family of C-type lectin receptors, which includes other members, such as the secreted phospholipase A 2 M-type receptor, the dendritic cell receptor DEC-205, and Endo180/urokinase plasminogen-activated receptor-associated protein.…”

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confidence: 99%

Mannose-Containing Molecular Patterns Are Strong Inducers of Cyclooxygenase-2 Expression and Prostaglandin E2 Production in Human Macrophages

Fernández

Alonso

Valera

et al. 2005

The Journal of Immunology

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The induction of cyclooxygenase-2 (COX-2) and the production of PGE2 in response to pathogen-associated molecular patterns decorated with mannose moieties were studied in human monocytes and monocyte-derived macrophages (MDM). Saccharomyces cerevisiae mannan was a robust agonist, suggesting the involvement of the mannose receptor (MR). MR expression increased along the macrophage differentiation route, as judged from both its surface display assessed by flow cytometry and the ability of MDM to ingest mannosylated BSA. Treatment with mannose-BSA, a weak agonist of the MR containing a lower ratio of attached sugar compared with pure polysaccharides, before the addition of mannan inhibited COX-2 expression, whereas this was not observed when agonists other than mannan and zymosan were used. HeLa cells, which were found to express MR mRNA, showed a significant induction of COX-2 expression upon mannan challenge. Conversely, mannan did not induce COX-2 expression in HEK293 cells, which express the mRNA encoding Endo180, a parent receptor pertaining to the MR family, but not the MR itself. These data indicate that mannan is a strong inducer of COX-2 expression in human MDM, most likely by acting through the MR route. Because COX-2 products can be both proinflammatory and immunomodulatory, these results disclose a signaling route triggered by mannose-decorated pathogen-associated molecular patterns, which can be involved in both the response to pathogens and the maintenance of homeostasis.

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Mannose receptor contribution to Candida albicans phagocytosis by murine E-clone J774 macrophages

Cited by 46 publications

References 70 publications

Specific Recognition of Candida albicans by Macrophages Requires Galectin-3 to Discriminate Saccharomyces cerevisiae and Needs Association with TLR2 for Signaling

Specific Recognition of Candida albicans by Macrophages Requires Galectin-3 to Discriminate Saccharomyces cerevisiae and Needs Association with TLR2 for Signaling

Innate immune cell response uponCandida albicansinfection

Mannose-Containing Molecular Patterns Are Strong Inducers of Cyclooxygenase-2 Expression and Prostaglandin E2 Production in Human Macrophages

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