Mannose binding lectin (mbl2) haplotype frequencies in solid organ transplant patients and correlation with MBL protein levels — Evaluation of complement-mediated effector pathway deficiency

Stevenson, Heather L.; Amador, Alexandra; McCue, Jennifer; Weppler, Deborah; Tryphonopoulos, Panagiotis; Roth, David; Ciancio, Gaetano; Burke, George W.; Chaparro, Sandra; Pham, Si M.; Tzakis, Andreas G.; Ruiz, Phillip

doi:10.1016/j.trim.2013.02.002

Cited by 6 publications

(10 citation statements)

References 48 publications

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“…MBL2 allele frequencies for comparisons were obtained from the following studies: Dutch [29], Inuit [30], Mozambican [25], South Korean [31], and those from the United States [32].…”

Section: Methodsmentioning

confidence: 99%

The association of mannose binding lectin genotype and immune response to Chlamydia pneumoniae: The Strong Heart Study

et al. 2019

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Cardiovascular disease (CVD) is an important contributor to morbidity and mortality in American Indian communities. The Strong Heart Study (SHS) was initiated in response to the need for population based estimates of cardiovascular disease in American Indians. Previous studies within SHS have identified correlations between heart disease and deficiencies in mannose binding lectin (MBL), a motif recognition molecule of the innate immune system. MBL mediates the immune response to invading pathogens including Chlamydia pneumoniae (Cp), which has also been associated with the development and progression of CVD. However, a link between MBL2 genotype and Cp in contributing to heart disease has not been established. To address this, SHS collected baseline Cp antibody titers (IgA and IgG) and MBL2 genotypes for common functional variants from 553 individuals among twelve participating tribes. A single nucleotide polymorphism (SNP) in the promoter, designated X/Y, correlated significantly with increased Cp IgG titer levels, whereas another promoter SNP (H/L) did not significantly influence antibody levels to Cp. Two variants within exon 1 of MBL2, the A and B alleles, also displayed significant association with Cp antibody titers. Some MBL2 genotypes were absent from the population, suggesting linkage disequilibrium may be operating within the SHS cohort. Additional factors, such as increasing age and socioeconomic status, were also associated with increased Cp IgG antibody titers. This study demonstrates that MBL2 genotype associates with immune reactivity to C. pneumoniae in the SHS cohort. Thus, MBL2 may contribute to the progression of cardiovascular disease (CVD) among American Indians indirectly through pathogen interactions in addition to its previously defined roles.

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“…MBL2 allele frequencies for comparisons were obtained from the following studies: Dutch [29], Inuit [30], Mozambican [25], South Korean [31], and those from the United States [32].…”

Section: Methodsmentioning

confidence: 99%

The association of mannose binding lectin genotype and immune response to Chlamydia pneumoniae: The Strong Heart Study

et al. 2019

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“…Serum MBL concentrations are largely influenced by genetic polymorphisms in the MBL2 gene. The most important polymorphisms affecting serum levels are three variant alleles in exon1 and two promoter SNPs affecting transcriptional activity (Table ) . Based on the serum MBL levels, genotype combinations are usually merged into a high‐ and low‐expressing genotype .…”

Section: Mbl Pathway Polymorphismsmentioning

confidence: 99%

“…The most important polymorphisms affecting serum levels are three variant alleles in exon1 and two promoter SNPs affecting transcriptional activity (Table ) . Based on the serum MBL levels, genotype combinations are usually merged into a high‐ and low‐expressing genotype . Similarly, serum ficolin‐2 (FCN2) and MBL‐associated serine protease 2 (MASP2) concentrations are largely affected by polymorphisms in their coding genes .…”

Section: Mbl Pathway Polymorphismsmentioning

confidence: 99%

“…Numerous polymorphisms in complement proteins have been identified thus far, including several polymorphisms that result in distinct functional differences (Figure ) . For instance, serum mannose‐binding lectin (MBL) levels are largely influenced by polymorphisms both in the promotor and the coding part of the MBL2 gene . A complete summary of the polymorphisms studied in renal transplantation is provided in Table .…”

Section: Introductionmentioning

confidence: 99%

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Complement Polymorphisms in Kidney Transplantation: Critical in Graft Rejection?

Michielsen

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Muskens

et al. 2017

American Journal of Transplantation

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The complement system, as part of the innate immune system, plays an important role in renal transplantation. Complement is involved in the protection against foreign organisms and clearance of apoptotic cells but can also cause injury to the renal allograft, for instance, via antibody binding or in ischemia-reperfusion injury. Numerous polymorphisms in complement factors have been identified thus far; some of them result in different functionalities or alter complement levels. In this review, we provide an overview of the literature on the role of complement polymorphisms in renal transplantation. Furthermore, we discuss functional complement polymorphisms that have not yet been investigated in kidney transplantation. By investigating multiple polymorphisms both in donor and recipient at the same time, a complotype can be constructed. Because the combination of multiple polymorphisms is likely to have a greater impact than a single one, this could provide valuable prognostic information.

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“…And many methods have been developed for clinical assay. Many ELISA kits can be selected for its analysis (Kwok, Vaida, Augst, Yu, & Singh, 2011;Stevenson et al, 2013;Suankratay, Zhang, Zhang, Lint, & Gewurz, 1998).…”

Section: Lectin Pathwaymentioning

confidence: 99%

Review on complement analysis method and the roles of glycosaminoglycans in the complement system

Ijaz

et al. 2015

Carbohydrate Polymers

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Mannose binding lectin (mbl2) haplotype frequencies in solid organ transplant patients and correlation with MBL protein levels — Evaluation of complement-mediated effector pathway deficiency

Cited by 6 publications

References 48 publications

The association of mannose binding lectin genotype and immune response to Chlamydia pneumoniae: The Strong Heart Study

The association of mannose binding lectin genotype and immune response to Chlamydia pneumoniae: The Strong Heart Study

Complement Polymorphisms in Kidney Transplantation: Critical in Graft Rejection?

Review on complement analysis method and the roles of glycosaminoglycans in the complement system

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