Mannitol, polyethylene glycol and NaCl induced polypeptide changes during in vitro culture of three tomato cultivars

Roy, R.; Agrawal, Veena; Gupta, S. C.

doi:10.1007/s10535-011-0132-5

Cited by 4 publications

(1 citation statement)

References 16 publications

(21 reference statements)

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“…The osmotic potential decreased gradually in plants treated with mannitol, showing that osmolyte accumulation increased over time. Mannitol is commonly used in many investigations to study the osmotic stress response of different plant species in vitro [ 9 , 10 , 11 , 12 , 13 , 14 , 15 ]. However, limited information is available on responses under mannitol-induced stress in tree crops, including the tea plant.…”

Section: Introductionmentioning

confidence: 99%

Biochemical and Genetic Responses of Tea (Camellia sinensis (L.) Kuntze) Microplants under Mannitol-Induced Osmotic Stress In Vitro

Самарина

Matskiv

Simonyan

et al. 2020

Plants

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Osmotic stress is a major factor reducing the growth and yield of many horticultural crops worldwide. To reveal reliable markers of tolerant genotypes, we need a comprehensive understanding of the responsive mechanisms in crops. In vitro stress induction can be an efficient tool to study the mechanisms of responses in plants to help gain a better understanding of the physiological and genetic responses of plant tissues against each stress factor. In the present study, the osmotic stress was induced by addition of mannitol into the culture media to reveal biochemical and genetic responses of tea microplants. The contents of proline, threonine, epigallocatechin, and epigallocatechin gallate were increased in leaves during mannitol treatment. The expression level of several genes, namely DHN2, LOX1, LOX6, BAM, SUS1, TPS11, RS1, RS2, and SnRK1.3, was elevated by 2–10 times under mannitol-induced osmotic stress, while the expression of many other stress-related genes was not changed significantly. Surprisingly, down-regulation of the following genes, viz. bHLH12, bHLH7, bHLH21, bHLH43, CBF1, WRKY2, SWEET1, SWEET2, SWEET3, INV5, and LOX7, was observed. During this study, two major groups of highly correlated genes were observed. The first group included seven genes, namely CBF1, DHN3, HXK2,SnRK1.1, SPS, SWEET3, and SWEET1. The second group comprised eight genes, viz. DHN2, SnRK1.3, HXK3, RS1, RS2,LOX6, SUS4, and BAM5. A high level of correlation indicates the high strength connection of the genes which can be co-expressed or can be linked to the joint regulons. The present study demonstrates that tea plants develop several adaptations to cope under osmotic stress in vitro; however, some important stress-related genes were silent or downregulated in microplants.

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Section: Introductionmentioning

confidence: 99%

Biochemical and Genetic Responses of Tea (Camellia sinensis (L.) Kuntze) Microplants under Mannitol-Induced Osmotic Stress In Vitro

Самарина

Matskiv

Simonyan

et al. 2020

Plants

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Virus-Induced Gene Silencing as a Scalable Tool to Study Drought Tolerance in Plants

George

Ruckle

Lloyd

2015

Methods in Molecular Biology

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Here we describe the methodology of using virus-induced gene silencing (VIGS) as a powerful and scalable tool to screen the function of genes that participate in adaptation to drought. Silencing of endogenous gene expression in Nicotiana benthamiana is achieved by systemic infection of the aerial parts of the plant with a virus engineered to contain homologous fragments of the target gene(s) of interest. Silenced plant material can be consistently produced with little optimization in less than 1 month without specialized equipment, using only simple cloning and transformation techniques. Although maximal silencing is localized to only a few leaves, when whole plants are subjected to water stress, the tissue from these silenced leaves can be characterized for physiological, biochemical, and transcriptional responses to determine the role of the candidate genes in drought tolerance.

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Glutathione and citric acid modulates lead- and arsenic-induced phytotoxicity and genotoxicity responses in two cultivars of Solanum lycopersicum L.

2017

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Mannitol, polyethylene glycol and NaCl induced polypeptide changes during in vitro culture of three tomato cultivars

Cited by 4 publications

References 16 publications

Biochemical and Genetic Responses of Tea (Camellia sinensis (L.) Kuntze) Microplants under Mannitol-Induced Osmotic Stress In Vitro

Biochemical and Genetic Responses of Tea (Camellia sinensis (L.) Kuntze) Microplants under Mannitol-Induced Osmotic Stress In Vitro

Virus-Induced Gene Silencing as a Scalable Tool to Study Drought Tolerance in Plants

Glutathione and citric acid modulates lead- and arsenic-induced phytotoxicity and genotoxicity responses in two cultivars of Solanum lycopersicum L.

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