Mannitol "In Vitro" Culture of Olea Europaea L. (Cv. Maurino)

Leva, A. R.; Petruccelli, R.; Bartolini, G.

doi:10.17660/actahortic.1994.356.7

Cited by 26 publications

(18 citation statements)

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“…In the multiplication media for olive micropropagation Leva et al (1992Leva et al ( , 1994) brought a significant contribution on this subject by replacing sucrose with mannitol. They observed that the increased culture media prices due to mannitol utilization were highly exceeded by the higher multiplication rates and explant growth capacity.…”

Section: Introductionmentioning

confidence: 99%

Coconut water and BAP successfully replaced zeatin in olive (Olea europaea L.) micropropagation

Peixe

Raposo

Lourenço

et al. 2007

Scientia Horticulturae

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The data presented report on trials conducted during 24 months using the Portuguese olive cultivar 'Galega vulgar'. The effectiveness of coconut water, BAP, or kinetin, as possible zeatin substitutes in olive micropropagation protocols, was investigated. In all stages of the micropropagation process, the mineral and vitamin formulation of olive medium (OM) was used. Regarding culture establishment the best results were achieved when 50 ml l À1 coconut water and 2.22 mM BAP were used as medium supplements. For the in vitro multiplication stage, the highest proliferation rates with an average of 3.4 new explants on each 30 days were achieved maintaining the coconut water concentration at 50 ml l À1 and increasing BAP up to 8.87 mM. The effects of IBA and activated charcoal on the in vitro root induction were also studied. Rooting rates of over 85% were obtained by basal immersion of the explants in IBA solution at 3 g l À1 for 10 s, followed by inoculation in the OM culture medium, added with 2 g l À1 of activated charcoal and without growth regulators. All in vitro rooted plants were transferred into Jiffy-Pots filled with vermiculite-perlite 3:1 (v/v) substrate. Those were subsequently wetted with the OM mineral solution, placed into polystyrene plates each one with 100 Jiffy-Pots capacity, which were transferred to traditional rooting mist benches, on a water-cooling equipped greenhouse. Such a simple acclimatization procedure allowed for 95% of plants survival. #

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Section: Introductionmentioning

confidence: 99%

Coconut water and BAP successfully replaced zeatin in olive (Olea europaea L.) micropropagation

Peixe

Raposo

Lourenço

et al. 2007

Scientia Horticulturae

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“…The results show the suitability of the MSM culture medium [25] as a substrate for the micropropagation of olive cultivars. The superior ability of MSM to support the physiological status of microshoots that is required for ex vitro rooting is supported by the results reported by Grigoriadou [8] on ex vitro rooting of cv.…”

Section: Discussionmentioning

confidence: 77%

“…Maurino were used for the ex vitro rooting tests. The explants were multiplied in MSM medium (Table 1; supplemented with 10 μM zeatin, 18.2 g L -1 mannitol and 2.2 g L -1 gelrite) [25]. The explants were subcultured regularly at 6-week intervals.…”

Section: Origin and Production Of Microshootsmentioning

confidence: 99%

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Innovative protocol for “ex vitro rooting” on olive micropropagation

Leva

2011

Open Life Sciences

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The commercial micropropagation of olive trees is currently limited by the production cost. An ex vitro method for olive microshoot rooting could reduce both the production cost per plant and the propagation time. In this study a successful ex vitro rooting protocol tested on seven olive cultivars is reported. The explants of cv. Maurino were collected from fifth, sixth, and seventh proliferative subcultures carried out on MSM medium, while for the other cultivars the explants were collected from only seventh proliferative subculture. Continuous light during the rooting phase was a prerequisite for the success of the ex vitro protocol. The best source of microshoots for a high rooting percentage was the seventh proliferative subculture. Cvs. Coratina, Maremmano, Maurino, Picholine, and S. Francesco showed high rooting percentages with a range of 62–76%; whereas for cvs. Correggiolo and Frantoio the experimental conditions need to be optimised. Up to 90% of the rooted microplants survived, and continuous growth of shoots was subsequently observed. The proposed protocol can be easily applied to several different olive cultivars to produce microplants by commercial laboratories. The approach makes olive micropropagation in the nursery industry both possible and profitable.

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“…During heat therapy, 2 to 2.5 cm long shoot tips are excised no earlier than three months from the beginning of the treatment. After surface-sterilization in 0,05% mercuric hydrochloride for 10 min, the shoot tips are placed in vitro in petri dishes on different media according to the cultivar [OM (Rugini, 1984); MSM media (Leva et al, 1994)] and grown at 24°C with a 16 h photoperiod.…”

Section: Strategies To Control Invasive Olive Pathogens and The Impormentioning

confidence: 99%