Mannheimia haemolytica and Pasteurella multocida in Bovine Respiratory Disease

Snyder, E. R.; Credille, Brent

doi:10.1016/j.cvfa.2020.02.001

Cited by 36 publications

(25 citation statements)

References 46 publications

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“…In this report, other forms of association were also identified, such as the triple association of BCoV, P. multocida , and M. haemolytica , double association of BCoV and P. multocida , and with a lower frequency, the double association of BCoV and M. haemolytica . The clinical disease caused by these bacteria in cattle results from a combination of stress, immunosuppression, and viral infection that can lead to abundant multiplication in the lower respiratory tract ( 38 ).…”

Section: Discussionmentioning

confidence: 99%

Bovine Coronavirus Co-infection and Molecular Characterization in Dairy Calves With or Without Clinical Respiratory Disease

et al. 2022

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Bovine respiratory disease (BRD) is considered a major cause of morbidity and mortality in young calves and is caused by a range of infectious agents, including viruses and bacteria. This study aimed to determine the frequency of viral and bacterial pathogens detected in calves with BRD from high-production dairy cattle herds and to perform the molecular characterization of N and S1 genes in identified bovine coronavirus (BCoV) strains. Nasal swabs were collected from 166 heifer calves, namely, 85 symptomatic and 81 asymptomatic calves aged between 5 and 90 days, from 10 dairy cattle herds. Nasal swabs were evaluated using molecular techniques for the identification of viruses (BCoV, bovine alphaherpesvirus 1, bovine viral diarrhea virus, bovine parainfluenza virus 3, and bovine respiratory syncytial virus) and bacteria (Pasteurella multocida, Mannheimia haemolytica, Histophilus somni, and Mycoplasma bovis). In addition, five and two BCoV-positive samples were submitted to N and S1 gene amplification and nucleotide sequencing, respectively. The frequency of diagnosis of BCoV was higher (56%, 93/166) than the frequency of P. multocida (39.8%, 66/166) and M. haemolytica (33.1%, 55/166). The three microorganisms were identified in the calves of symptomatic and asymptomatic heifer calve groups. All other pathogens included in the analyses were negative. In the phylogenetic analysis of the S1 gene, the Brazilian strains formed a new branch, suggesting a new genotype, called # 15; from the N gene, the strains identified here belonged to cluster II. This study describes high rates of BCoV, P. multocida, and M. haemolytica in heifer calves from high-production dairy cattle herds with BRD. Additionally, the molecular characterization provides evidence that the circulating BCoV strains are ancestrally different from the prototype vaccine strains and even different BCoV strains previously described in Brazil.

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Section: Discussionmentioning

confidence: 99%

Bovine Coronavirus Co-infection and Molecular Characterization in Dairy Calves With or Without Clinical Respiratory Disease

et al. 2022

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“…Concerning production systems, a particularity of our study is that we included dairy and beef cattle, and we showed that the prevalence of FHN bronchopneumonia was not linked to breed type. Further studies concerning the potential genetic or virulence particularities of M. haemolytica strains affecting adult cattle and immunity are needed [ 33 , 34 , 35 , 36 ].…”

Section: Discussionmentioning

confidence: 99%

Infectious Bovine Respiratory Diseases in Adult Cattle: An Extensive Necropsic and Etiological Study

Dorso

Rouault

Barbotin

et al. 2021

Animals

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In young cattle, bovine respiratory disease (BRD) is a major cause of death and Mannheimia haemolytica is a frequent pathogen. Knowledge of fatal BRD in adult cattle is more limited. We assessed the importance of infectious BRD as a cause of death in adult cattle and determined the associated pathogens. We analyzed data from 737 adult cattle necropsies at the Pathology Unit for Large Animals at Oniris, Nantes, France over a 6 year period (2013–2019). Each carcass was subjected to a complete necropsy. Lungs showing macroscopic lesions were classified into three categories: infectious primary pulmonary (IPP) lesions, thromboembolic pneumonia (TEP) and others (aspiration pneumonia, verminous pneumonia, and local extension of an extra-pulmonary inflammatory process). Half of the lungs with IPP macroscopic lesions were sampled for histology and submitted for polymerase chain reaction. BRD was the second leading cause of death (15.7%) after digestive diseases (32.2%). A strong predominance of IPP lesions (42.3%) and TEP lesions (39.6%) was also demonstrated. In IPP macroscopic lesions, fibrinous, hemorrhagic and/or hecrotic (FHN) bronchopneumonia accounted for 77.6% of macroscopic lesions. Mannheimia haemolytica was significantly associated with FHN bronchopneumonia macroscopic lesions. This study suggests that Mannheimia haemolytica should be included in the differential diagnosis of BRD in adult cattle.

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“…Based on previous studies, P. multocida serogroup A is considered the major pathogen of BRD (2,19,20). The persistent respiratory tract infection caused by P. multocida serogroup A has always been a huge issue for the cattle industry.…”

Section: Discussionmentioning

confidence: 99%

“…In recent years, the high incidence of bovine respiratory diseases (BRD) has seriously affected the cattle breeding industry worldwide, and these diseases mainly occur in fattening cattle and calves during long distance transportation, and cause a lack of energy and appetite, accompanied by cough, runny nose, and dyspnea (1). In North America, BRD accounts for ∼75% of all disease incidence and 50% of all mortality in cattle farms, with an even higher prevalence (nearly 90%) in calves (2).…”

Section: Introductionmentioning

confidence: 99%

Genomic and Transcriptomic Analysis of Bovine Pasteurella multocida Serogroup A Strain Reveals Insights Into Virulence Attenuation

et al. 2021

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Pasteurella multocida is one of the primary pathogens of bovine respiratory disease (BRD), and causes huge losses in the cattle industry. The Pm3 strain was a natural isolate, which is a strong form of pathogen and is sensitive to fluoroquinolones antibiotics. A high fluoroquinolone resistant strain, Pm64 (MIC = 64 μg/mL), was formed after continuous induction with subinhibitory concentration (1/2 MIC) of enrofloxacin, with the enhanced growth characteristics and large attenuation of pathogenicity in mice. This study reports the whole genome sequence and the transcription profile by RNA-Seq of strain Pm3/Pm64. The results showed an ineffective difference between the two strains at the genome level. However, 32 genes could be recognized in the gene islands (GIs) of Pm64, in which 24 genes were added and 8 genes were lost. Those genes are involved in DNA binding, trehalose metabolism, material transportation, capsule synthesis, prophage, amino acid metabolism, and other functions. In Pm3 strain, 558 up-regulated and 568 down-regulated genes were found compared to Pm64 strain, from which 20 virulence factor-related differentially expressed genes (DEGs) were screened. Mainly differentially transcribed genes were associated with capsular polysaccharide (CPS), lipopolysaccharide (LPS), lipooligosaccharide (LOS). Iron utilization, and biofilm composition. We speculated that the main mechanism of virulence attenuation after the formation of resistance of Pm64 comes from the change of the expression profile of these genes. This report elucidated the toxicity targets of P. multocida serogroup A which provide fundamental information toward the understanding of the pathogenic mechanism and to decreasing antimicrobial drugs resistance.

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Mannheimia haemolytica and Pasteurella multocida in Bovine Respiratory Disease

Cited by 36 publications

References 46 publications

Bovine Coronavirus Co-infection and Molecular Characterization in Dairy Calves With or Without Clinical Respiratory Disease

Bovine Coronavirus Co-infection and Molecular Characterization in Dairy Calves With or Without Clinical Respiratory Disease

Infectious Bovine Respiratory Diseases in Adult Cattle: An Extensive Necropsic and Etiological Study

Genomic and Transcriptomic Analysis of Bovine Pasteurella multocida Serogroup A Strain Reveals Insights Into Virulence Attenuation

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