Mannheimia cairinae sp. nov., a novel species of Mannheimia obtained from Muscovy ducks (Cairina moschata) and reclassification of Mannheimia ovis as heterotypic synonym of Mannheimia pernigra

Abstract

During a screening study for Pasteurella multocida in two unrelated flocks of Muscovy ducks pharyngeal and cloacal swabs were collected. A total of 59 Pasteurellaceae -like isolates sharing the same colony morphology were subcultured and subsequently characterized. Colonies on bovine blood agar were nonhaemolytic, regular, circular, slightly raised, shiny, intransparent with an entire margin, greyish and had an unguent-like consistency. Isolate AT1T was characterized by 16S rRNA gene sequencing and showed the highest similarity of 96.1 % to the type strain of Mannheimia caviae and 96.0 % to the type strain of Mannheimia bovis , respectively. In addition, rpoB and recN gene sequences also showed the highest similarity to the genus Mannheimia . The phylogenetic comparison of concatenated conserved protein sequences also showed a unique position of AT1T compared to other species of Mannheimia . Full phenotypic characterization of the isolates showed that between two ( Mannheimia ruminalis ) and 10 ( Mannheimia glucosida ) phenotypic characteristics separate the taxon isolated from Muscovy ducks from the accepted species of Mannheimia . Whole genomic sequences of two strains analysed by the type strain genome server showed the highest similarity of 24.9 % to the genome of the type strain of Pasteurella multocida and 23.0 % to the genome of the type strain of Mannheimia haemolytica . The species Mannheimia cairinae sp. nov. is proposed based on the phenotypic and genotypic similarity to Mannheimia as well as differences to the other validly published species of the genus. The leukotoxin protein was not predicted in the genome of AT1T. The G+C content of the type strain of M. cairinae sp. nov., AT1T (=CCUG 76754T=DSM 115341T) is 37.99 mol%, calculated from the whole genome. The investigation further proposes that Mannheimia ovis is reclassified as a later heterotypic synonym of Mannheimia pernigra , since M. ovis and M. pernigra are closely genetically related, and M. pernigra was validly published before M. ovis .

Mannheimia indoligenes sp. nov., proposed for clade V organisms of Mannheimia

“…Phenotypic characters obtained are in accordance with those of Pasteurellaceae and the genus Mannheimia [8]. Isolates of clade V can only be separated from M. varigena by production of indole, while two to seven characters separate clade V from M. haemolytica (2), M. glucosida (3), M. granulomatis (3), M. ruminalis (3), M. caviae (7) [9], M. pernigra (6) [10], and M. cairinae (6) [11] ().…”

“…Phenotypic characteristics separating the species of MannheimiaSpecies: 1, Mannheimia indoligenes sp. nov.; 2, Mannheimia cairinae[11]; 3, Mannheimia pernigra[10]; 4, Mannheimia haemolytica[2]; 5, Mannheimia glucosida[2]; 6, Mannheimia granulomatis[2]; 7, Mannheimia ruminalis[2]; 8, Mannheimia varigena[2]; 9, Mannheimia caviae[9]; 10, Mannheimia bovis[26]*. +, 90 % or more of the strains positive within 1-2 days; (+), 90 % or more of the strains positive within 3-14 days (late positive); -, less than 10 % of the strains are positive within 14 days; d, 11-89 % of the strains are positive; w, weak positive.…”