Mannan-Modified Solid Lipid Nanoparticles for Targeted Gene Delivery to Alveolar Macrophages

Yu, Wangyang; Liu, Chunxi; Liu, Yu; Zhang, Na; Xu, Wenfang

doi:10.1007/s11095-010-0149-z

Cited by 127 publications

(82 citation statements)

References 38 publications

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“…So nanoparticles (5200 nm) can significantly accumulate in tumor by ''filtration'' mechanism (Xu et al, 2009). Small particles are also minimal endocytosis by macrophages, so destruction and clearance could be minimized (Yu et al, 2010). A mean diameter lower than 200 nm and a PDI lower than 0.2 were considered desirable to propose the formulation for in vitro and in vivo experiments (Accardo et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Which one performs better for targeted lung cancer combination therapy: pre- or post-bombesin-decorated nanostructured lipid carriers?

Li²

2015

Drug Delivery

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Purpose: The co-delivery of gene and drugs has the potential to treat cancer. The aim of this study was to compare post-bombesin decorated nanostructured lipid carriers (NLC) carrying both doxorubicin (DOX) and DNA with pre-bombesin decorated NLC for lung cancer therapy. Methods: Post-bombesin decorated NLC were prepared by two steps. First, DOX and DNA-loaded NLC (DOX-DNA-NLC) was prepared. Second, Bombesin-NH 2 (BN-NH 2 ) was added into DOX-DNA-NLC to react with stearic acid-polyethylene glycol-COOH (SA-PEG-COOH) loaded in NLC. Pre-bombesin decorated NLC were prepared by two steps. First, Bombesin (BN)-conjugated ligands were synthesized. Second, DOX and DNA were loaded into BN decorated NLC. Their average size, zeta potential, drug and gene loading were evaluated. NCl-H460 human non-small lung cancer cells (NCl-H460 cells) were used for the testing of in vitro transfection efficiency and in vitro cytotoxicity. In vivo transfection efficiency and anti-tumor effect of NLC were evaluated on mice bearing NCl-H460 cells model. Results: Post-bombesin decorated NLC has a particle size of 128 nm, DOX encapsulation efficiency (EE) of 85% and DNA EE of 91%. Pre-bombesin decorated NLC has a particle size of 101 nm, DOX EE of 86% and DNA EE of 92%. Post-bombesin decorated NLC displayed more stable and remarkably higher transfection efficiency and better anti-tumor ability than pre-bombesin decorated NLC both in vitro and in vivo. Conclusion: Post-bombesin decorated NLC could function as better carriers to improve the cell targeting and nuclear targeting ability. The resulting nanomedicine could be a promising active targeting drug/gene therapeutic system for lung cancer therapy.

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Section: Discussionmentioning

confidence: 99%

Which one performs better for targeted lung cancer combination therapy: pre- or post-bombesin-decorated nanostructured lipid carriers?

Li²

2015

Drug Delivery

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“…The IC 50 value of RGD-TMZ/NLCs was 2-fold over RGD-TMZ/NLCs and 10-fold over TMZ solution in reducing viability of malignant glioma cells, accounting for the highest antitumor activity. Cytotoxicity deeply influenced the ability of the nanocarriers (Yu et al, 2010). The results demonstrated the higher cytotoxicity of NLCs formulas than free drug solution.…”

Section: In Vitro Cytotoxicitymentioning

confidence: 95%

Novel RGD containing, temozolomide-loading nanostructured lipid carriers for glioblastoma multiforme chemotherapy

Song

Mao

et al. 2015

Drug Delivery

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Context: Glioblastoma multiforme (GBM) is the most common malignant brain tumor originating in the central nervous system. Efficient delivery of therapeutic molecules to the cells and tissues is a difficult challenge. Objective: Arginine-glycine-aspartic acid peptide (RGD)-modified nanostructured lipid carriers (NLCs) were used for the delivery of temozolomide (TMZ) into the GBM to provide a new paradigm in gliomatosis cerebri treatment. Methods:RGD-conjugated polyethylene glycol-b-distearoylphosphatidylethanolamine (PEG-DSPE) was synthesized. RGD containing, TMZ-loaded NLCs (RGD-TMZ/NLCs) were prepared. Their particle size, zeta potential, drug encapsulation efficiency (EE) and drug release behavior were evaluated. In vitro cytotoxicity study of TMZ/NLCs was tested in U87 malignant glioma cells (U87MG cells). In vivo antitumor efficacy of the carriers was evaluated on mice bearing GBM model. Results: The U87MG cells were successfully inhibited by RGD-TMZ/NLCs in vitro. RGD-TMZ/NLCs also displayed the highest antitumor efficacy in vivo than all the other formulations used for comparison. Conclusion: RGD-TMZ/NLCs were efficient in selective delivery of TMZ into U87MG cells, and inhibition efficacy is high. These RGD-modified vectors could be a superior drug delivery nanosystem to achieve therapeutic efficacy, and this research could be a new promising strategy for treatment in malignant gliomatosis cerebri.

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“…[20][21][22] Stearic acid 50 mg and injectable soya lecithin 30 mg were accurately weighed and dissolved in 10 mL of acetone. The organic phase was added dropwise into the 0.2% dimethyldioctadecylammonium bromide solution, under stirring at 600 rpm and at room temperature.…”

Section: Preparation Of Cationic Slnmentioning

confidence: 99%

“…19 It has been shown that human and murine macrophages express the mannose receptor on their surface, and several studies have confirmed the feasibility of using mannose or mannan-modified nanocarriers to target macrophages. 20 In the present study, a novel conjugated ligand, mannanpolyethylene glycol-L-α-phosphatidylethanolamine (MN-PEG-PE) was synthesized, and MN-PEG-PE-modified SLN were investigated. In this study we compared the two strategies for modification using the same ligands (MN-PEG-PE) in an attempt to identify which kind of nanomedicine is preferable for targeted gene therapy, ie, premodified or postmodified gene-loaded SLN/DNA.…”

Section: Introductionmentioning

confidence: 99%

Comparison of two kinds of nanomedicine for targeted gene therapy: premodified or postmodified gene delivery systems

Jiang¹,

Sun²,

Yin³

et al. 2012

IJN

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Background:The applications of ligand-polyethylene glycol (PEG)-modified nanocarriers have now emerged, as well as recognized strategies to provide the vectors with active targeting properties. In this research, premodification and postmodification were compared using the same ligand, ie, a novel conjugated mannan-containing PEG and L-α-phosphatidylethanolamine (PE). Methods: Premodified and postmodified solid lipid nanoparticles were prepared and the characteristics of the two kinds of vehicles were evaluated. The modified vectors were then administered intravenously to rats and the in vivo targeting behavior of the complexes was investigated in liver macrophages. Results: By carefully formulating the carriers with an optimal ratio of mannan-containing PEG-PE, postmodified vehicles displayed more efficient gene expression in rat Kupffer cells both in vitro and in vivo. Conclusion: Postmodified gene carriers are superior to premodified gene vectors, although the latter is also promising for targeted gene delivery. This discovery could guide our future research.

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Mannan-Modified Solid Lipid Nanoparticles for Targeted Gene Delivery to Alveolar Macrophages

Abstract: These results demonstrated the active targeting ability of this kind of mannan-modified DNA-loaded vehicles, which may have great potential for targeted gene delivery.

Cited by 127 publications

References 38 publications

Which one performs better for targeted lung cancer combination therapy: pre- or post-bombesin-decorated nanostructured lipid carriers?

Which one performs better for targeted lung cancer combination therapy: pre- or post-bombesin-decorated nanostructured lipid carriers?

Novel RGD containing, temozolomide-loading nanostructured lipid carriers for glioblastoma multiforme chemotherapy

Comparison of two kinds of nanomedicine for targeted gene therapy: premodified or postmodified gene delivery systems

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