Manipulations in HIWI level exerts influence on the proliferation of human non-small cell lung cancer cells

Wang, Yuguang; Liu, Jia; Wu, Guangyao; Yang, Fang

doi:10.3892/etm.2016.3106

Cited by 9 publications

(7 citation statements)

References 38 publications

(44 reference statements)

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“…The protein and mRNA levels of Hiwi were significantly upregulated in the intra-tumor non-small cell lung cancer (NSCLC) samples. The expression of Hiwi had close relationship with cancer cell proliferation [27]. In pancreatic cancer, on the other hand, altered mRNA expression of hiwi had poorer clinical prognosis [28].…”

Section: Pirnas In Cancermentioning

confidence: 98%

The disease-related biological functions of PIWI-interacting RNAs (piRNAs) and underlying molecular mechanisms

Han

2019

ExRNA

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More than a decade ago, PIWI-interacting RNA (piRNA) was discovered almost simultaneously by four different research groups. The length of this type of single stranded noncoding RNA is 24~31 nucleotides (nt), with most of the piRNAs fall into the range of 29~30 nt. PiRNAs form specific RNA-induced silencing complex with PIWI subfamily proteins, which is how piRNA got its name. PiRNAs are initially famous for the crucial roles they played in germline cells. Binding with PIWI family proteins, piRNAs is able to affect methylation of genomic DNA in germline cells and, therefore, maintaining genomic stability and suppressing transposons. Since mammalian PIWI subfamily proteins are mainly germline specific, it was once thought that piRNAs might only function in the gonadal cells. However, evidence from the later research suggest that piRNAs are broadly expressed in many kinds of somatic cells and are involved in numerous pathological condition far beyond those have been reported in the germline. For example, piRNAs were discovered to be abnormally expressed in several kinds of cancers. PiRNAs are also shown to be promising prognostic markers for various types of cancers. Interestingly, a recent research indicated that piRNAs are also regulators for pancreatic beta cell function. PiRNAs are promising regulators for the development of type 2 diabetes. From a disease-oriented point of view, this review will focus on both confirmed and proposed biological functions of piRNAs mostly in those fields beyond germline cells. Meanwhile, some of the underlying molecular mechanisms will also be mentioned.

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Section: Pirnas In Cancermentioning

confidence: 98%

The disease-related biological functions of PIWI-interacting RNAs (piRNAs) and underlying molecular mechanisms

Han

2019

ExRNA

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“…Therefore, it was suggested that the PIWIL1 gene could be considered as a molecular target for the treatment of lung cancer, and the use of the PIWIL1 gene silencing technology is considered as a promising treatment [28, 37]. In an investigation, the PIWIL1 expression was manipulated to evaluate its role in the proliferation of NSCLC [38]. Researchers examined the PIWIL1 expression at both levels of protein and mRNA of samples collected from 57 patients with NSCLC by gain of function and loss of function strategies.…”

Section: Piwi Proteins In Lung Cancermentioning

confidence: 99%

“…Researchers examined the PIWIL1 expression at both levels of protein and mRNA of samples collected from 57 patients with NSCLC by gain of function and loss of function strategies. The proliferation of human A549 cell line was also evaluated using colony formation assays and cell counting kit-8 [38]. Results showed that protein and mRNA expression levels of the PIWIL1 had considerably upregulation in NSCLC specimens.…”

Section: Piwi Proteins In Lung Cancermentioning

confidence: 99%

“…Results showed that protein and mRNA expression levels of the PIWIL1 had considerably upregulation in NSCLC specimens. Also, the knockout and overexpression of the PIWIL1 were associated with the inhibition and promotion of cell proliferation, respectively [38]. Xie et al [39] showed that the PIWIL1 overexpression facilitated proliferation, migration and invasion in lung cancer cells.…”

Section: Piwi Proteins In Lung Cancermentioning

confidence: 99%

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Epigenetic roles of PIWI proteins and piRNAs in lung cancer

Fathizadeh

Asemi

2019

Cell Biosci

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Lung cancer is one of very important malignancies which are related to high mobility and mortality in the world. Despite several efforts for improving diagnosis and treatment strategies of lung cancer, finding and developing new and effective therapeutic and diagnostic are needed. A variety of internal and external factors could be involved in lung cancer pathogenesis. Among internal factors, epigenetic mechanisms have been emerged as very important players in the lung cancer. Non-coding RNAs is known as one of epigenetic regulators which exert their effects on a sequence of cellular and molecular mechanisms. P-element induced wimpy testis (PIWI)-interacting RNAs (piRNAs or piR) is one of small non-coding RNAs that the deregulation of these molecules is associated with initiation and progression of different cancers such as lung cancer. Several activities are related to PIWI/piRNA pathway such as suppression of transposons and mobile genetic elements. In vitro and in vivo studies demonstrated the upregulation or downregulation of PIWI proteins and piRNAs could lead to the increasing of cell proliferation, apoptosis reduction and promoting tumor growth in the lung cancer. Hence, PIWI proteins and piRNA could be introduced as new diagnostic and therapeutic biomarkers in the lung cancer therapy. Herein, we have focused on PIWI proteins and piRNA functions and their impact on the progression of lung cancer.

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“…In vitro and in vivo studies demonstrated that PIWIL1 upregulation contributes to increased tumorigenesis, resistance to chemotherapeutic drugs, acquisition of self-renewal abilities and elevated expression of stem-cell-related transcription markers including octamer-binding protein 4 (OCT4), homeobox protein NANOG (NANOG) and Polycomb complex protein BMI1 ( 41 ). The levels of PIWIL1 protein and transcript were significantly upregulated in intratumor tissue from patients with non-small cell lung cancer (NSCLC), compared with in peritumor tissue ( 42 ). In addition, using gain-of-function and loss-of-function strategies, a positive association between the expression of PIWIL1 and proliferation of the NSCLC cell line A549 was identified ( 42 ).…”

Section: Piwi-pirna In Cancer Developmentmentioning

confidence: 99%

The meaning of PIWI proteins in cancer development

et al. 2017

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Abstract. Cancer is a histologically and genetically heterogeneous population of tumor cells that exhibits distinct molecular profiles determined by epigenetic alterations. P-element-induced wimpy testis (PIWI) proteins in complex with PIWI-interacting RNA (piRNA) have been previously demonstrated to be involved in epigenetic regulation in germline cells. Recently, reactivation of PIWI expression, primarily PIWI-like protein 1 and 2, through aberrant DNA methylation resulting in genomic silencing has been identified in various types of tumors. It has been suggested that the PIWI-piRNA complex contributes to cancer development and progression by promoting a stem-like state of cancer cells, or cancer stem cells (CSCs). It has been identified that CSCs represent the cells that have undergone epithelial-mesenchymal transition (EMT) and acquired metastatic capacities. However, the molecular association between the EMT process and the stem-cell state remains unclear. Further extensive characterization of CSCs in individual types of tumors is required to identify specific markers for the heterogeneous population of CSCs and therefore selectively target CSCs. Previous studies indicate a reciprocal regulation between PIWI proteins and a complex signaling network linking markers characterized for CSCs and transcription factors involved in EMT. In the present review, studies of PIWI function are summarized, and the potential involvement of PIWI proteins in cancer development and progression is discussed. Contents1. Epigenetic alterations in cancer 2. P-element-induced wimpy testis (PIWI) proteins and PIWI-interacting RNAs (piRNAs) 3. PIWI-piRNA in cancer development 4. Characteristics of cancer stem cells (CSCs) 5. EMT in CSC development 6. Conclusions Epigenetic alterations in cancerCancer has been described as a set of diseases driven by progressive genetic abnormalities, including mutations in tumor suppressor genes, oncogenes and chromosomal abnormalities, and by aberrant epigenetic alterations (1). Epigenetic alterations identified in cancer include global DNA hypomethylation, particularly in repetitive regions, but also in the intronic and the coding regions of genes. These alterations may result in the reactivation of transposons, the loss of chromosomal stability and imprinting patterns. Another epigenetic modification is gene-specific DNA hypermethylation, particularly in promoter regions of tumor suppressor genes, deregulation of histone modification patterns and consequently alterations in gene expression. Additionally, small non-coding RNA (ncRNA) deregulation has been studied in detail in various types of cancer in recent years (1). All these alterations drive the transformation of wild-type cells into highly malignant tumor consisting of neoplastic cells with metastatic potential and unlimited proliferation capacities (1,2). P-element-induced wimpy testis (PIWI) proteins and PIWI-interacting RNAs (piRNAs)Currently, three major classes of small regulatory RNAs have been identified: microRNAs, small interfering...

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Manipulations in HIWI level exerts influence on the proliferation of human non-small cell lung cancer cells

Cited by 9 publications

References 38 publications

The disease-related biological functions of PIWI-interacting RNAs (piRNAs) and underlying molecular mechanisms

The disease-related biological functions of PIWI-interacting RNAs (piRNAs) and underlying molecular mechanisms

Epigenetic roles of PIWI proteins and piRNAs in lung cancer

The meaning of PIWI proteins in cancer development

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