2011
DOI: 10.1007/s00253-011-3145-2
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Manipulation of quorum sensing regulation in Pseudomonas fluorescens NCIMB 10586 to increase mupirocin production

Abstract: Transcription of the 74 kb Pseudomonas fluorescens mupirocin [pseudomonic acid (PA)] biosynthesis cluster depends on quorum sensing-dependent regulation via the LuxI/LuxR homologues MupI/MupR. To facilitate analysis of novel PAs from pathway mutants, we investigated factors that affect mup gene expression. First, the signal produced by MupI was identified as N-(3-oxodecanoyl)homoserine lactone, but exogenous addition of this molecule did not activate mupirocin production prematurely nor did expression of mupI … Show more

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Cited by 17 publications
(11 citation statements)
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“…S20). These data are in accordance with previous reports that mupirocin is under QS control by 3-oxo-C10 AHL (44,45).…”
Section: Identification Isolation and Structure Elucidation Of Mupisupporting
confidence: 94%
“…S20). These data are in accordance with previous reports that mupirocin is under QS control by 3-oxo-C10 AHL (44,45).…”
Section: Identification Isolation and Structure Elucidation Of Mupisupporting
confidence: 94%
“…Truncated forms of mupirocin and related metabolites with C 7 and C 5 hydroxy acids have also been observed in both mutant strains and when the quorum sensing regulation mechanism has been manipulated to increase mupirocin production. 29…”
Section: The Biosynthesis Of Thiomarinol a And Related Metabolitesmentioning
confidence: 99%
“…However, some Burkholderia species contain an additional copy of M , which is in a non-canonical arrangement, either because there are one to five additional genes betweem R and M ( M 2 topology, found in B. pseudomallei 1106a, B. pseudomallei 1710b, B. pseudomallei 668, B. pseudomallei K96243, B. thailandensis E264), or because the otherwise constitutive R gene is missing in the immediate vicinity of the MI tandem ( M 3 topology, B. ambipharia AMMD, and MC40-6). On the other hand, P. fluorescens NCIMB 10586 contains a gene coding for an enzyme, mupX in the X position [21]. …”
Section: Genomic Topologies Of Ahl-driven Qs Circuitsmentioning
confidence: 99%
“…M was found to negatively regulate QS in P. fuscovaginae [24]. Finally, mupX of P. fluorescens NCIMB10586 is an amidase-hydrolase that was shown to degrade the AHL signal produced by the same species thereby decreasing the QS response [21]. …”
Section: The X Genesmentioning
confidence: 99%