Manipulation of plant architecture and ﬂowering time by down-regulation of the GRAS transcription factor SlGRAS26 in Solanum lycopersicum

Zhou, Shengen; Hu, Zongli; Li, Fenfen; Yu, Xiaohui; Naeem, Muhammad; Zhang, Yanjie; Chen, Guoping

doi:10.1016/j.plantsci.2018.03.017

Cited by 27 publications

(21 citation statements)

References 90 publications

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“…Changes in biosynthesis or perception of plant hormones, such as gibberellin (GA) 6 , brassinosteroid 7 , auxin 8 and strigolactone 9 can cause plant dwarfism. Additionally, abnormal expression of transcription factors, such as HD-Zip II 10 , WOX 11 , AP2 12 and GRAS 13 can also lead to dwarf architecture in plants.…”

Section: Introductionmentioning

confidence: 99%

A mutation in the intron splice acceptor site of a GA3ox gene confers dwarf architecture in watermelon (Citrullus lanatus L.)

Sun

Zhang

Fan

et al. 2020

Sci Rep

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Dwarf architecture is an important trait associated with plant yield, lodging resistance and labor cost. Here, we aimed to identify a gene causing dwarfism in watermelon. The ‘w106’ (dwarf) and ‘Charleston Gray’ (vine) were used as parents to construct F1 and F2 progeny. Dwarf architecture of ‘w106’ was mainly caused by longitudinal cell length reduction and was controlled by a single recessive gene. Whole-genome sequencing of two parents and two bulk DNAs of F2 population localized this gene to a 2.63-Mb region on chromosome 9; this was further narrowed to a 541-kb region. Within this region, Cla015407, encoding a gibberellin 3β-hydroxylase (GA3ox), was the candidate gene. Cla015407 had a SNP mutation (G → A) in the splice acceptor site of the intron, leading to altered splicing event and generating two splicing isoforms in dwarf plants. One splicing isoform retained the intron sequences, while the other had a 13-bp deletion in the second exon of GA3ox transcript, both resulting in truncated proteins and loss of the functional Fe2OG dioxygenase domain in dwarf plants. RNA-Seq analysis indicated that expression of Cla015407 and other GA biosynthetic and metabolic genes were mostly up-regulated in the shoots of dwarf plants compared with vine plants in F2 population. Measurement of endogenous GA levels indicated that bioactive GA4 was significantly decreased in the shoots of dwarf plants. Moreover, the dwarf phenotype can be rescued by exogenous applications of GA3 or GA4+7, with the latter having a more distinct effect than the former. Subcellular localization analyses of GA3ox proteins from two parents revealed their subcellular targeting in nucleus and cytosol. Here, a GA3ox gene controlling dwarf architecture was identified, and loss function of GA3ox leads to GA4 reduction and dwarfism phenotype in watermelon.

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Section: Introductionmentioning

confidence: 99%

A mutation in the intron splice acceptor site of a GA3ox gene confers dwarf architecture in watermelon (Citrullus lanatus L.)

Sun

Zhang

Fan

et al. 2020

Sci Rep

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“…GRAS proteins share conserved domains in their C-terminus, comprised LHR I, VHIID, LHR II, PFYRE and SAW 1,7–9 , however, the N-terminus of GRAS proteins show a great divergence, which may result to the functional specificity of each protein 10 . Although metazoan STATs share similar domain organization with plant GRAS, it is lack of enough support for the hypothesis that GRAS proteins are plant STATS 11 .…”

Section: Introductionmentioning

confidence: 99%

“…Up to now, more than a dozen of GRAS gene family have been identified, including Arabidopsis thaliana 1,7,12 , Rice 7,13 , Populus 14 , pine 15 , Chinese cabbage 16 , tobacco 17 , tomato 18,19 , Prunus mume 20 , Jatropha curcas L. 21 , Lotus japonicus 22 , grapevine 23,24 , Nelumbo nucifera 25 , Ricinus communis 26 , Betula kirghisorum 27 , Isatis indigotica 28 , apple 29 , Zea mays L. 30 , Medicago truncatula 31 , Camellia sinensis 32 and Gossypium hirsutum 33 . The plant-specific GRAS family of proteins function as transcriptional regulators and play critical roles in development and signaling, such as in signal transduction (gibberellin signal transduction 2,34 , phytochrome A signal transduction) 35,36 , stress responses 23,37–40 , meristem formation and maintenance 8,41–44 and promoting flowering 9 .…”

Section: Introductionmentioning

confidence: 99%

Genome-wide Identification, Classification, Expression and Duplication Analysis of GRAS Family Genes in Juglans regia L.

Quan

Niu

Zhou

et al. 2019

Sci Rep

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Fifty-two GRAS genes are identified in walnut genome. Based on the evolutionary relationship and motif analysis, the walnut GRAS gene family was divided into eight subfamilies, and the sequence features analysis of Jr GRAS proteins showed that the Jr GRAS protein sequences were both conserved and altered during the evolutionary process. Gene duplication analysis indicated that seven GRAS genes in walnut have orthologous genes in other species, and five of them occurred duplicated events in walnut genome. Expression pattern analysis of the GRAS family genes in walnut showed that two Jr GRAS genes ( JrCIGRa-b and JrSCL28a ) were differentially expressed between flower bud and leaf bud (p < 0.01), and two JrGRAS genes ( JrCIGRa - b and JrSCL1 3 b - d ) were differentially expressed between the different development stages of flower buds transition (p < 0.01), besides, three hub genes ( JrGAIa , JrSCL3f and JrSHRc ) were identified by co-expression analysis, which suggested these GRAS genes may play an important role in regulating the development of apical meristem in walnut. This study laid a foundation for further understanding of the function of GRAS family genes in walnut.

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“…Downregulation of SlGRAS26 altered plant phase transition and morphological traits in tomato. SlGRAS26 showed a response to ABA, GA, IAA, dehydration, and abiotic stresses [49]. There are eleven GRAS proteins that belong to the PAT1 subfamily.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of SlGRAS7 Affects Multiple Behaviors Leading to Confer Abiotic Stresses Tolerance and Impacts Gibberellin and Auxin Signaling in Tomato

Habib

Waseem

et al. 2019

International Journal of Genomics

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Abiotic stresses remain the key environmental issues that reduce plant development and therefore affect crop production. Transcription factors, such as the GRAS family, are involved in various functions of abiotic stresses and plant growth. The GRAS family of tomato (Solanum lycopersicum), SlGRAS7, is described in this study. We produced overexpressing SlGARS7 plants to learn more about the GRAS transcription factors. Plants overexpressing SlGARS7 (SlGRAS7-OE) showed multiple phenotypes related to many behaviors, including plant height, root and shoot length, and flowering time. We observed that many genes in the SlGRAS7-OE seedlings that are associated with auxin and gibberellin (GA) are downregulated and have altered sensitivity to GA3/IAA. SlGRAS7 was upregulated during abiotic stresses following treatment with sodium chloride (NaCl) and D-mannitol in the wild-type (WT) tomato. Tomato plants overexpressing SlGRAS7 showed more resistance to drought and salt stress comparison with WT. Our study of SlGRAS7 in tomato demonstrates how GRAS showed an integrative role, improving resistance to abiotic stresses and enhancing gibberellin/auxin signaling through reproductive as well as vegetative processes.

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Manipulation of plant architecture and ﬂowering time by down-regulation of the GRAS transcription factor SlGRAS26 in Solanum lycopersicum

Cited by 27 publications

References 90 publications

A mutation in the intron splice acceptor site of a GA3ox gene confers dwarf architecture in watermelon (Citrullus lanatus L.)

A mutation in the intron splice acceptor site of a GA3ox gene confers dwarf architecture in watermelon (Citrullus lanatus L.)

Genome-wide Identification, Classification, Expression and Duplication Analysis of GRAS Family Genes in Juglans regia L.

Overexpression of SlGRAS7 Affects Multiple Behaviors Leading to Confer Abiotic Stresses Tolerance and Impacts Gibberellin and Auxin Signaling in Tomato

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