Manipulating the nature of embryonic mitotic waves

Hayden, Luke; Hur, Woonyung; Vergassola, Massimo; Talia, Stefano Di

doi:10.1016/j.cub.2022.10.014

Cited by 8 publications

(15 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Early patterns (T 1 : 0-60 min) resemble phase waves: a roughly uniform upswing in activity, and preservation of local peaks and spatial gradients (Fig. 1c, top), sharing similar features as those reported in Hayden et al 17 . Conversely, at late times (T 2 : 1000-1200 min), the system exhibits clearly linear, trigger-wave-like fronts, characterized by traveling pulses (Fig.…”

Section: Mitotic Waves Transition From Fast Phase Waves To Slower Tri...supporting

confidence: 82%

“…Furthermore, this and other work highlight that phase wave properties are not robust to heterogeneity as they do not correspond to an actual attracting system solution. In Hayden et al 17 , the authors also show embryos displaying a nuclear density gradient in the syncytial embryo can lead to trigger rather than sweep or phase waves. We demonstrate a similar effect of disrupting homogeneity both by adding nuclei to the homogeneous system and by driving it explicitly with CSF.…”

Section: Discussionmentioning

confidence: 97%

“…At early times, the patterns we observe resemble phase waves highlighted in Drosophila embryos (Figure 1C, Top). Taking a cue from Hayden et al (17), when we plot the FRET ratio spatial profile over consecutive frames for early cycles, we see a roughly uniform upswing in activity, with local peaks preserved, largely preserving spatial gradients (Figure 1C, Top). While this does not equate to a strict proof for the existence of sweep waves in Xenopus-higher time resolution and parameter scaling are required to be certain-the similarity is striking.…”

Section: Mitotic Waves Transition From Fast Phase Waves To Slower Tri...mentioning

confidence: 89%

See 2 more Smart Citations

Mitotic waves in frog egg extracts: Transition from phase waves to trigger waves

Puls,

Ruiz-Reynés,

Tavella

et al. 2024

Preprint

View full text Add to dashboard Cite

The cell cycle clock network centers on the cyclin-dependent kinase (Cdk1), such that its oscillatory rising and falling activity directs the cell through a series of steps which define one mitotic cycle. When a collection of these oscillators couple, they can synchronize. In particular, early embryogenesis is marked by a series of synchronous and fast cell divisions across the length of the embryo in various systems, e.g. Drosophila (approximately 0.5 mm in length) and Xenopus (approximately 1.2 mm in diameter). However, the large size of these embryos implies a faster coordinating effect than diffusion can control by itself. Two types of waves have been suggested as a mechanism for such spatial coordination: phase waves and trigger waves, which can be distinguished by both the speeds at which they propagate and the biochemical mechanisms behind their formation. Here, by using Xenopus laevis egg extracts and a Cdk1 FRET sensor to study the time dependence of mitotic waves, we show a transition from phase waves to trigger waves for the first time. We show how the addition of nuclei entrains the system more quickly to the trigger wave regime. We also demonstrate that the system is entrained almost immediately when metaphase-arrested extracts initiate the waves from the boundary. Finally, we complement experiments with computational modeling showing how the observed cell cycle period and wave speed depend on transient dynamics and underlying cell cycle oscillator properties. Our work argues that the transition of fast phase waves to slower trigger waves in the spatial coordination of the cell division cycle occurs as a transient effect due to the time required for trigger waves to entrain the system. Moreover, we argue that trigger waves play a role in spatial coordination, and that both phase and trigger waves are a manifestation of a common biological process undergoing these transient dynamics.

show abstract

Section: Mitotic Waves Transition From Fast Phase Waves To Slower Tri...supporting

confidence: 82%

Section: Discussionmentioning

confidence: 97%

Section: Mitotic Waves Transition From Fast Phase Waves To Slower Tri...mentioning

confidence: 89%

See 1 more Smart Citation

Mitotic waves in frog egg extracts: Transition from phase waves to trigger waves

Puls,

Ruiz-Reynés,

Tavella

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…The influence of multiple bistable switches on mitotic waves remains to be investigated Chang and Ferrell Jr (2013); Afanzar et al (2020); Nolet et al (2020). The ability to gradually increase (decrease) the presence of the (cyclin B, active Cdk1) switch by b y , as well as control the time-scale separation by ϵ y and ϵ z , could also be interesting to study transitions from trigger waves to sweep waves Vergassola et al (2018); Hayden et al (2022); Di Talia and Vergassola (2022).…”

Section: Discussionmentioning

confidence: 99%

Cell cycle oscillations driven by two interlinked bistable switches

Parra‐Rivas¹,

Ruiz-Reynés

Gelens

2023

Preprint

View full text Add to dashboard Cite

Regular transitions between interphase and mitosis during the cell cycle are driven by changes in the activity of the enzymatic protein complex cyclin B with cyclin-dependent kinase 1 (Cdk1). At the most basic level, this cell cycle oscillator is driven by negative feedback: active cyclin B - Cdk1 activates the Anaphase-Promoting Complex - Cyclosome, which triggers the degradation of cyclin B. Such cell cycle oscillations occur fast and periodically in the early embryos of the frog Xenopus laevis, where several positive feedback loops leading to bistable switches in parts of the regulatory network have been experimentally identified. Here, we build cell cycle oscillator models to show how single and multiple bistable switches in parts of the underlying regulatory network change the properties of the oscillations and how they can confer robustness to the oscillator. We present a detailed bifurcation analysis of these models.

show abstract

“…As a result of this reduced nuclear movement, nuclear density across the embryos is highly non-uniform. In turn, this results in asynchronous cell cycles prior to the maternal-to-zygotic transition (MZT), which demonstrates the functional role of the flows [20, 28, 29].…”

mentioning

confidence: 99%

Two-fluid dynamics and micron-thin boundary layers shape cytoplasmic flows in earlyDrosophilaembryos

López

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Cytoplasmic flows are widely emerging as key functional players in development. In earlyDrosophila embryos, flows drive the spreading of nuclei across the embryo. Here, we combine hydrodynamic modeling with quantitative imaging to develop a two-fluid model that features an active actomyosin gel and a passive viscous cytosol. Gel contractility is controlled by the cell cycle oscillator, the two fluids being coupled by friction. In addition to recapitulating experimental flow patterns, our model explains observations that remained elusive, and makes a series of new predictions. First, the model captures the vorticity of cytosolic flows, which highlights deviations from Stokes' flow that were observed experimentally but remained unexplained. Second, the model reveals strong differences in the gel and cytosol motion. In particular, a micron-sized boundary layer is predicted close to the cortex, where the gel slides tangentially whilst the cytosolic flow cannot slip. Third, the model unveils a mechanism that stabilizes the spreading of nuclei with respect to perturbations of their initial positions. This self-correcting mechanism is argued to be functionally important for proper nuclear spreading. Fourth, we use our model to analyze the effects of flows on the transport of the morphogen Bicoid, and the establishment of its gradients. Finally, the model predicts that the flow strength should be reduced if the shape of the domain is more round, which is experimentally confirmed inDrosophilamutants. Thus, our two-fluid model explains flows and nuclear positioning in earlyDrosophila, while making predictions that suggest novel future experiments.

show abstract

Manipulating the nature of embryonic mitotic waves

Cited by 8 publications

References 27 publications

Mitotic waves in frog egg extracts: Transition from phase waves to trigger waves

Mitotic waves in frog egg extracts: Transition from phase waves to trigger waves

Cell cycle oscillations driven by two interlinked bistable switches

Two-fluid dynamics and micron-thin boundary layers shape cytoplasmic flows in earlyDrosophilaembryos

Contact Info

Product

Resources

About