Manipulating the insulating post arrangement in DC-biased AC-iEK devices to improve microparticle separations

Nasir Ahamed, Nuzhet Nihaar; Mendiola-Escobedo, Carlos A.; Perez-Gonzalez, Victor H.; Lapizco-Encinas, Blanca H.

doi:10.1039/d3an02160b

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2024

DOI: 10.1039/d3an02160b

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Manipulating the insulating post arrangement in DC-biased AC-iEK devices to improve microparticle separations

Nuzhet Nihaar Nasir Ahamed,

Carlos A. Mendiola-Escobedo,

Victor H. Perez-Gonzalez

et al.

Abstract: This study proposes a strategy for improving the separation resolution of binary microparticle mixtures by modifying the post array arrangement in insulator-based electrokinetic devices.

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Development of a DC-Biased AC-Stimulated Microfluidic Device for the Electrokinetic Separation of Bacterial and Yeast Cells

Nasir Ahamed,

Mendiola-Escobedo,

Perez-Gonzalez

et al. 2024

Biosensors

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Electrokinetic (EK) microsystems, which are capable of performing separations without the need for labeling analytes, are a rapidly growing area in microfluidics. The present work demonstrated three distinct binary microbial separations, computationally modeled and experimentally performed, in an insulator-based EK (iEK) system stimulated by DC-biased AC potentials. The separations had an increasing order of difficulty. First, a separation between cells of two distinct domains (Escherichia coli and Saccharomyces cerevisiae) was demonstrated. The second separation was for cells from the same domain but different species (Bacillus subtilis and Bacillus cereus). The last separation included cells from two closely related microbial strains of the same domain and the same species (two distinct S. cerevisiae strains). For each separation, a novel computational model, employing a continuous spatial and temporal function for predicting the particle velocity, was used to predict the retention time (tR,p) of each cell type, which aided the experimentation. All three cases resulted in separation resolution values Rs>1.5, indicating complete separation between the two cell species, with good reproducibility between the experimental repetitions (deviations < 6%) and good agreement (deviations < 18%) between the predicted tR,p and experimental (tR,e) retention time values. This study demonstrated the potential of DC-biased AC iEK systems for performing challenging microbial separations.

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Development of a DC-Biased AC-Stimulated Microfluidic Device for the Electrokinetic Separation of Bacterial and Yeast Cells

Nasir Ahamed,

Mendiola-Escobedo,

Perez-Gonzalez

et al. 2024

Biosensors

Self Cite

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show abstract

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Manipulating the insulating post arrangement in DC-biased AC-iEK devices to improve microparticle separations

Abstract: This study proposes a strategy for improving the separation resolution of binary microparticle mixtures by modifying the post array arrangement in insulator-based electrokinetic devices.

Cited by 1 publication

References 43 publications

Development of a DC-Biased AC-Stimulated Microfluidic Device for the Electrokinetic Separation of Bacterial and Yeast Cells

Development of a DC-Biased AC-Stimulated Microfluidic Device for the Electrokinetic Separation of Bacterial and Yeast Cells

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