Manipulating the generation of Ca-alginate microspheres using microfluidic channels as a carrier of gold nanoparticles

Huang, Keng‐Shiang; Lai, Tzung-Heng; Lin, Yu-Cheng

doi:10.1039/b606424h

Cited by 122 publications

(89 citation statements)

References 14 publications

(14 reference statements)

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“…However, in most reports, alginate droplets thus formed are crosslinked off-chip, falling from the microfluidic chip into a setting bath of calcium ions. 12 Using such an approach, both standardization and sterility are difficult to achieve. Zhang et al 13 have succeeded in curing alginate droplets in situ, in a microfluidic device, using undecanol as a solvent for calcium ions.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic chip-based synthesis of alginate microspheres for encapsulation of immortalized human cells

et al. 2007

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Cellular transplantation is a promising technology with great clinical potential in regenerative medicine and disease management. However, effective control over patient immunological response is essential. The encapsulation of cells within hydrogel microspheres is an increasingly prevalent method for the protection of cellular grafts from immune rejection. Microfluidic "chip" reactors present elegant solutions to several capsule generation issues, including the requirement for intercapsule uniformity, high reproducibility, and sterile, good manufacturing practice compliance. This study presents a novel method for the on-chip production of stable, highly monodisperse alginate microspheres and demonstrates its utility in the encapsulation of an immortalized human-derived cell line. Four populations of immortalized human embryonic kidney cells ͑HEK293͒ were encapsulated on chip within monodisperse alginate capsules. Cell viability measurements were recorded for each of the four encapsulated populations for 90 days.

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Section: Introductionmentioning

confidence: 99%

Microfluidic chip-based synthesis of alginate microspheres for encapsulation of immortalized human cells

et al. 2007

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“…Highly monodispersed droplets [13][14][15][16][17][18][19] and spherical polymeric microparticles [20][21][22][23][24] can be obtained by using the hydrodynamic flow focusing method or the cross-flow shear method in T-junction microchannels. Several groups [25][26][27][28][29][30] have attempted to utilize such techniques to produce monodispersed Ca-alginate hydrogel beads. Huang et al [25,26] firstly produced monodispersed emulsions of Na-alginate droplets in an organic phase by using the hydrodynamic flow focusing method.…”

Section: Introductionmentioning

confidence: 99%

“…Several groups [25][26][27][28][29][30] have attempted to utilize such techniques to produce monodispersed Ca-alginate hydrogel beads. Huang et al [25,26] firstly produced monodispersed emulsions of Na-alginate droplets in an organic phase by using the hydrodynamic flow focusing method. Then the formed emulsions were dripped into a solution containing 20 % calcium chloride (CaCl 2 ) to create Ca-alginate microparticles.…”

Section: Introductionmentioning

confidence: 99%

Controllable Preparation of Monodispersed Calcium Alginate Microbeads in a Novel Microfluidic System

Tan

et al. 2008

Chem Eng & Technol

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This work describes a novel method for the preparation of monodispersed calcium alginate microbeads in a microfluidic device. A novel microfluidic device comprising of two cross-junction channels and a synthesizing channel was designed by controlling the mixing condition with laminar flow and two-phase droplet flow in microfluidic devices. At the first junction, the side two streams, sodium alginate aqueous solution and calcium chloride aqueous solution, were separated to prevent contact by the injection of deionized water in the middle channel. Then the aqueous mixture solution was induced into monodispersed droplets by a focusing sheath of immiscible oil phase at the second junction. The formed droplets were solidified in situ in the synthesizing channel with the rapid mixing of reactants in the droplets. The size of the Ca-alginate microbeads can be controlled from 50-300 lm in diameter with a variation of less than 2 %, simply by changing the two-phase flow ratio. The proposed microfluidic platform is capable of generating uniform emulsions and has the advantages of in situ solidification and good control of particle size.

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“…31 While early work produced alginate microgels through the extrusion of alginate droplets into crosslinking baths, [32][33][34] recent studies have applied droplet microfluidics to create more monodisperse alginate microgels. [35][36][37][38][39] Several strategies for achieving gelation within an emulsion template have been investigated, including internal and external gelation. For internal gelation, a sequestered source of divalent cations is dispersed throughout the alginate solution and released after emulsification.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic generation of alginate microgels for the controlled delivery of lentivectors

et al. 2016

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Microgels fabricated through distinct microfluidic procedures encapsulate and release functioning lentivectors in a controlled manner.Please check this proof carefully. Our staff will not read it in detail after you have returned it.Translation errors between word-processor files and typesetting systems can occur so the whole proof needs to be read. Please pay particular attention to: tabulated material; equations; numerical data; figures and graphics; and references. If you have not already indicated the corresponding author(s) please mark their name(s) with an asterisk. Please e-mail a list of corrections or the PDF with electronic notes attached -do not change the text within the PDF file or send a revised manuscript. Corrections at this stage should be minor and not involve extensive changes. All corrections must be sent at the same time.Please bear in mind that minor layout improvements, e.g. in line breaking, table widths and graphic placement, are routinely applied to the final version.Please note that, in the typefaces we use, an italic vee looks like this: n, and a Greek nu looks like this: n.We will publish articles on the web as soon as possible after receiving your corrections; no late corrections will be made.Please return your final corrections, where possible within 48 hours of receipt, by e-mail to: materialsB@rsc.orgQueries for the attention of the authors However, achieving safe, localized, and sufficient gene expression remain key challenges for effective lentivectoral therapy. Localized and efficient gene expression can be promoted by developing material systems to deliver lentivectors. Here, we address the utility of microgel encapsulation as a strategy for the controlled release of lentivectors. Three distinct routes for ionotropic gelation of alginate were incorporated into microfluidic templating to create lentivector-loaded microgels. Comparisons of the three microgels revealed marked differences in mechanical properties, crosslinking environment, and ultimately lentivector release and functional gene expression in vitro. Gelation with chelated calcium demonstrated low utility for gene delivery due to a loss of lentivector function with acidic gelation conditions. Both calcium carbonate gelation, and calcium chloride gelation, preserved lentivector function with a more sustained transduction and gene expression over 4 days observed with calcium chloride gelated microgels. The validation of these two strategies for lentivector microencapsulation may provide a platform for controlled gene delivery.

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Manipulating the generation of Ca-alginate microspheres using microfluidic channels as a carrier of gold nanoparticles

Cited by 122 publications

References 14 publications

Microfluidic chip-based synthesis of alginate microspheres for encapsulation of immortalized human cells

Microfluidic chip-based synthesis of alginate microspheres for encapsulation of immortalized human cells

Controllable Preparation of Monodispersed Calcium Alginate Microbeads in a Novel Microfluidic System

Microfluidic generation of alginate microgels for the controlled delivery of lentivectors

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