Manipulating secondary metabolism in cultured plant cells

Yeoman, M. M.; Yeoman, C. L.

doi:10.1111/j.1469-8137.1996.tb04921.x

Cited by 98 publications

(55 citation statements)

References 135 publications

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“…Highly contradictory results were reported in literature in regards to the effect of medium composition on SM production in plant cell culture (Dicosmo and Towers, 1984;Yeoman and Yeoman, 1996). For example, in Catharanthus roseus cells an increased alkaloid production level was observed when P concentration was either increased or reduced in the culture medium.…”

Section: Could Contradictory Results Be Related To Plant Cells Nutritmentioning

confidence: 82%

“…For example, in Catharanthus roseus cells an increased alkaloid production level was observed when P concentration was either increased or reduced in the culture medium. Similarly, a reduction of the nitrogen concentration in the culture medium often resulted in an increase in SM production level, however an increased N concentration was also reported to promote SM production in a cell suspension of this same specie (Yeoman and Yeoman, 1996, and references therein).…”

Section: Could Contradictory Results Be Related To Plant Cells Nutritmentioning

confidence: 90%

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Critical influence of Eschscholzia californica cells nutritional state on secondary metabolite production

Lamboursain

Jolicœur

2005

Biotech & Bioengineering

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In this study, we investigated the influence of initial internal nutrient concentrations at the time of elicitation on the ability of Eschscholzia californica (EC) cells to produce alkaloids. Three EC cell suspensions cultivated in culture media differing in their PO4(3-) and NO3- contents were sampled daily for 12 days and analyzed for extracellular and intracellular nutrient concentrations. The ability of the cells to produce alkaloids was tested along the three cell suspension cultures. Sampled cells were then further cultured for 7 days in a production medium containing the elicitor and an extraction resin. The alkaloid production of the cells was measured 7 days post-elicitation. In the low-N medium, starch, glucose, and phosphate contents in the biomass was increased by 470, 1624 and 70%, respectively, 10 days after inoculation compared to the control culture in standard B5 medium. Cell concentration was significantly reduced from 10.3 to 8.6 millions cell/mL on this low-N medium compared to the control, nevertheless alkaloid production was multiplied by 39 at day 10 when cells were elicited. Cells grown on the low-N or low-P media accumulated 83% and 188% more carbon, respectively, than control cells at the end of the culture. This intracellular C was mainly stored in the form of starch in low-P medium and both in the form of starch and glucose in the low-N medium. The ability of EC cells to produce alkaloids upon elicitation was shown to be strongly dependent on the initial intracellular C and P content at the time of elicitation. This suggests that reproducibility and productivity during EC cell culture could be enhanced by manipulating the intracellular C and P content at the time of elicitation.

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Section: Could Contradictory Results Be Related To Plant Cells Nutritmentioning

confidence: 82%

Section: Could Contradictory Results Be Related To Plant Cells Nutritmentioning

confidence: 90%

Critical influence of Eschscholzia californica cells nutritional state on secondary metabolite production

Lamboursain

Jolicœur

2005

Biotech & Bioengineering

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“…Con certeza, el análisis de estos datos nos lleva a entender que la concentración adecuada para mostrar un determinado efecto depende de la especie vegetal utilizada y el tipo de extracto, donde la actividad sobre los microorganismos no se deba a la acción de un único principio activo sino al efecto sinergístico de varios de ellos que en la planta se encuentran en proporción minoritaria (Leatemia & Isman 2004;Davicino et al 2007) Referente a los extractos de CH 2 Cl 2 :MeOH (2:1), obtenidos de plantas in vitro, la inhibición en el crecimiento fue 100% en concentraciones de 0,5 a 1,5 mg/mL pero solamente para T. rubrum y M. canis; para M. gypseum la inhibición en el crecimiento (70-95%) fue ligeramente inferior (Tabla 6). Estos resultados abren la posibilidad de biosintetizar a gran escala los compuestos antifúngicos de P. tuberculatum mediante el establecimiento de suspensiones celulares, tal como se viene realizando en varias especies de importancia medicinal (Yeoman & Yeoman 1996). En el caso de especies de Piper, en suspensiones celulares de P. cernuum fueron producidas las feniletilaminas dopamina y tiramina, y en P. crassinervium predominaron cuatro alkamidas, entre ellas la inédita 2,3,4-trimetoxy-N-metil-aristolactamano, en tanto, que en plantas adultas fenilpropanoides y ácidos benzoico prenilados, respectivamente, fueron los mayores compuestos detectados (Danelutte et al 2005;Kato & Furlán 2007).…”

Section: Resultados Y Discusiónunclassified

In vitro antifungal activity of crude extracts of Piper tuberculatum

et al. 2009

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ResumenEn la medicina tradicional Peruana Piper tuberculatum Jacq. (Piperaceae) es utilizado en humanos y animales domésticos como antiinflamatorio y desinfectante de heridas. Piper tuberculatum contiene las amidas isobutí-licas, pirrolidina, dihidropiridona y piperidina. El objetivo de este trabajo fue investigar la actividad antifúngica de los extractos crudos de inflorescencias, hojas y tallos de plantas silvestres, obtenidos con CH 2 Cl 2 :MeOH (2:1), EtOH y decocción y de plantas in vitro obtenido con CH 2 Cl 2 :MeOH (2:1). Los extractos crudos exhibieron actividad antifúngica sobre Trichophyton rubrum, Microsporum canis y M. gypseum. La concentración inhibitoria mínima (CIM) observada con los extractos CH 2 Cl 2 :MeOH (2:1), EtOH y decocción, sobre Trichophyton rubrum, Microsporum canis y M. gypseum fue 0,1 mg/mL para inflorescencias y hojas, y 0,1 a 0,5 mg/mL para tallos. En plantas in vitro la inhibición en el crecimiento de T. rubrum y M. canis fue 100% en 0,5 mg/mL y para M. gypseum fue 95% en 1,5 mg/mL de concentración. AbstractPiper tuberculatum Jacq. (Piperaceae) is used in traditional Peruvian medicine as anti-inflammatory and disinfectant of wounds in humans and domestic animals. This species contains amides bearing isobutyl, pyrrolidine, dihydropyridone and piperidine moieties. The aim of this work was to investigate antifungal activity of crude extracts from the spikes, leaves and stems of wild plants extracted with CH 2 Cl 2 :MeOH (2:1), EtOH, decoction, and in vitro plants extracted with CH 2 Cl 2 :MeOH (2:1). The crude extracts showed antifungal activity on Trichophyton rubrum, Mycosporum canis y M. gypseum. The minimum inhibitory concentration (MIC) observed with CH 2 Cl 2 :MeOH (2:1), EtOH and decoctions extracts against T. rubrum, M. canis and M. gypseum was 0,1 mg/mL for spikes and leaves, and 0,1 to 0,5 mg/mL for stems. The inhibition of growth using in vitro plants on T. rubrum and M. canis was 100% in 0,5 mg/mL, and 95% on M. gypseum 95% using 1,5 mg/mL of concentration.

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“…The environmental factors involved include the temperature and illumination of the culture room, agitation process and incubation period of the cultures [4]. For the initiation of callus culture, the following factors are important-: the origin of explants used for the establishment of callus culture, the cellular/tissue differentiation status, external plant growth regulators, culture media and culture conditions [46]. Cellular competence to plant hormones is understood as the status in which a cell must possess the ability to perceive a transducer and respond to a signal [47].…”

Section: Organogenesismentioning

confidence: 99%