Manipulating Nutritional Conditions and Salinity‐Gradient Stress for Enhanced Lutein Production in Marine Microalga <i>Chlamydomonas</i> sp.

Xie, Youping; Lu, Kongyong; Zhao, Xurui; Ma, Ruijuan; Chen, Jianfeng; Ho, Shih‐Hsin

doi:10.1002/biot.201800380

Cited by 25 publications

(14 citation statements)

References 51 publications

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“…However, higher productivities presented by Vaquero et al [41] and Casal et al [42] for C. onubensis (410 mg L -1 day -1 ) and Coccomyxa acidophila (130-250 mg L -1 day -1 ), suggest that further improvements can be made to enhance biomass productivity at low pH. Increases in biomass productivity could be obtained by utilizing different growing media [43]. The BBM utilized in this experiment had an original pH of 6.6 whereas modi ed acid media (MAM), with an original pH of 4 [44], has been successfully used as a source of nutrients for acidophilic microalgae such as Chlamydomonas acidophila [45].…”

Section: Discussionmentioning

confidence: 93%

Dark Stress to Improve Lipid Quantity and Quality in Acid-Tolerant Microalgae Exposed to Simulated (6% CO2) Flue Gas

Desjardins

Laamanen

Muinonen

et al. 2021

Preprint

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The use of CO2 rich industrial flue gases to support cultivation of microalgae to produce lipids for biofuel and other applications is an increasingly researched option. However, this approach presents a challenge, as whilst flue gasses typically contain 6-10% CO2, excessive medium acidification can be caused by the presence of NOx and SO2. The use of acidophilic or acid-tolerant species is a possible solution, but little is known about these microalgae. In this study we investigated the growth of a bioprospected acid-tolerant mixed photosynthetic green microalgae culture (91% dominated by a single Coccomyxa sp. taxon) at pH 2.5 and fed with a simulated flue gas containing 6% CO2 and 94% N2. At the end of the exponential growth phase, lipid accumulation and profiles, and the elemental composition of biomass were analysed over one week during which biomass was exposed to either continued light-dark cycle conditions or continual dark conditions. After three days of dark stress, the biomass consisted of approximately 28% of lipids, which was 42% higher than at the end of the exponential phase and 55% higher than the maximum lipid content achieved under light/dark conditions. Oleic acid (C18:1), pentadecanoic acid (C15:0), and palmitic acid (C16:0) were the dominant fatty acids at the end of the exponential phase, and light-dark and dark-treated biomass, respectively. Dark stress conditions favoured polyunsaturated fatty acid production and showed an increase in nitrogen content. This suggests that the use of dark stress to stimulate production of desirable lipids is a no-cost alternative to other commonly used stressors.

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Section: Discussionmentioning

confidence: 93%

Dark Stress to Improve Lipid Quantity and Quality in Acid-Tolerant Microalgae Exposed to Simulated (6% CO2) Flue Gas

Desjardins

Laamanen

Muinonen

et al. 2021

Preprint

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“…For instance, lutein content decreased when nitrogen concentration exceeded 1000 mg/L in Chlamydomonas sp. JSC4 [91], while it stabilized at 9.65 mg/g under excessive nitrogen concentration in Chlorella sorokiniana FZU60 [92]. At the other end, nitrogen starvation induces the accumulation of storage carbohydrates and lipids, which may be due to the transformation of protein or peptides into these energy-rich metabolites [93].…”

Section: Nitrogenmentioning

confidence: 98%

“…Similarly, a salinity-shift strategy has been applied to biodiesel production in D. salina KSA-HS022 [104] and lutein production in Chlamydomonas sp. JSC4 [91].…”

Section: Cultivation Strategies Based On Environmental Conditionsmentioning

confidence: 99%

Comprehensive Utilization of Marine Microalgae for Enhanced Co-Production of Multiple Compounds

Wang

Chua

et al. 2020

Marine Drugs

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Marine microalgae are regarded as potential feedstock because of their multiple valuable compounds, including lipids, pigments, carbohydrates, and proteins. Some of these compounds exhibit attractive bioactivities, such as carotenoids, ω-3 polyunsaturated fatty acids, polysaccharides, and peptides. However, the production cost of bioactive compounds is quite high, due to the low contents in marine microalgae. Comprehensive utilization of marine microalgae for multiple compounds production instead of the sole product can be an efficient way to increase the economic feasibility of bioactive compounds production and improve the production efficiency. This paper discusses the metabolic network of marine microalgal compounds, and indicates their interaction in biosynthesis pathways. Furthermore, potential applications of co-production of multiple compounds under various cultivation conditions by shifting metabolic flux are discussed, and cultivation strategies based on environmental and/or nutrient conditions are proposed to improve the co-production. Moreover, biorefinery techniques for the integral use of microalgal biomass are summarized. These techniques include the co-extraction of multiple bioactive compounds from marine microalgae by conventional methods, super/subcritical fluids, and ionic liquids, as well as direct utilization and biochemical or thermochemical conversion of microalgal residues. Overall, this review sheds light on the potential of the comprehensive utilization of marine microalgae for improving bioeconomy in practical industrial application.

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“…In this regard, LUT is accumulated on a large scale in several species of Chlorella such as C. sorokiniana, Chromochloris zoofingiensis (formerly Chlorella zoofingiensis), and Auxenochlorella prothecoides (formerly Chlorella protothecoides) [38], as well as in Dunaliella salina [39], the strain Chlamydomonas sp. JSC4 [40], and Tetraselmis suecica [41].…”

Section: Luteinmentioning

confidence: 99%

Anti-Inflammatory and Anticancer Effects of Microalgal Carotenoids

et al. 2021

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Acute inflammation is a key component of the immune system’s response to pathogens, toxic agents, or tissue injury, involving the stimulation of defense mechanisms aimed to removing pathogenic factors and restoring tissue homeostasis. However, uncontrolled acute inflammatory response may lead to chronic inflammation, which is involved in the development of many diseases, including cancer. Nowadays, the need to find new potential therapeutic compounds has raised the worldwide scientific interest to study the marine environment. Specifically, microalgae are considered rich sources of bioactive molecules, such as carotenoids, which are natural isoprenoid pigments with important beneficial effects for health due to their biological activities. Carotenoids are essential nutrients for mammals, but they are unable to synthesize them; instead, a dietary intake of these compounds is required. Carotenoids are classified as carotenes (hydrocarbon carotenoids), such as α- and β-carotene, and xanthophylls (oxygenate derivatives) including zeaxanthin, astaxanthin, fucoxanthin, lutein, α- and β-cryptoxanthin, and canthaxanthin. This review summarizes the present up-to-date knowledge of the anti-inflammatory and anticancer activities of microalgal carotenoids both in vitro and in vivo, as well as the latest status of human studies for their potential use in prevention and treatment of inflammatory diseases and cancer.

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Manipulating Nutritional Conditions and Salinity‐Gradient Stress for Enhanced Lutein Production in Marine Microalga Chlamydomonas sp.

Cited by 25 publications

References 51 publications

Dark Stress to Improve Lipid Quantity and Quality in Acid-Tolerant Microalgae Exposed to Simulated (6% CO2) Flue Gas

Dark Stress to Improve Lipid Quantity and Quality in Acid-Tolerant Microalgae Exposed to Simulated (6% CO2) Flue Gas

Comprehensive Utilization of Marine Microalgae for Enhanced Co-Production of Multiple Compounds

Anti-Inflammatory and Anticancer Effects of Microalgal Carotenoids

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