Manipulating human dendritic cell phenotype and function with targeted porous silicon nanoparticles

Stead, Sebastian O.; McInnes, Steven J. P.; Kireta, Svjetlana; Rose, P.D.; Jesudason, Shilpanjali; Rojas-Canales, Darling; Warther, David; Cunin, Frédérique; Durand, J.O.; Drogemuller, Chris; Carroll, Robert; Coates, P. Toby; Voelcker, Nicolas H.

doi:10.1016/j.biomaterials.2017.11.017

Cited by 36 publications

(40 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The characterization of pSi NPs was performed in our earlier work [ 38 ]. Briefly, the particle size was found to be in the range of 161 ± 58 nm and the particles possessed a pore size of 33 ± 7 nm.…”

Section: Resultsmentioning

confidence: 99%

Electrospun Composites of Polycaprolactone and Porous Silicon Nanoparticles for the Tunable Delivery of Small Therapeutic Molecules

et al. 2018

View full text Add to dashboard Cite

This report describes the use of an electrospun composite of poly(ε-caprolactone) (PCL) fibers and porous silicon (pSi) nanoparticles (NPs) as an effective system for the tunable delivery of camptothecin (CPT), a small therapeutic molecule. Both materials are biodegradable, abundant, low-cost, and most importantly, have no known cytotoxic effects. The composites were treated with and without sodium hydroxide (NaOH) to investigate the wettability of the porous network for drug release and cell viability measurements. CPT release and subsequent cell viability was also investigated. We observed that the cell death rate was not only affected by the addition of our CPT carrier, pSi, but also by increasing the rate of dissolution via treatment with NaOH. This is the first example of loading pSi NPs as a therapeutics nanocarrier into electronspun PCL fibers and this system opens up new possibilities for the delivery of molecular therapeutics.

show abstract

Section: Resultsmentioning

confidence: 99%

Electrospun Composites of Polycaprolactone and Porous Silicon Nanoparticles for the Tunable Delivery of Small Therapeutic Molecules

et al. 2018

View full text Add to dashboard Cite

show abstract

“…In addition to silica nanoparticles, silicon nanoparticles could also be designed as excellent drug‐delivery vehicles for biomedical applications . Lee and co‐workers reported degradable hollow mesoporous silicon/carbon nanoparticles for pH‐responsive, imaging‐guided chemothermal combination therapy ( Figure a,b) .…”

Section: Silica Nanoparticlesmentioning

confidence: 99%

Degradability and Clearance of Inorganic Nanoparticles for Biomedical Applications

et al. 2019

View full text Add to dashboard Cite

Inorganic nanoparticles with tunable and diverse properties hold tremendous potential in the field of nanomedicine, while having non‐negligible toxicity concerns in healthy tissues/organs that have resulted in their restricted clinical translation to date. In the past decade, the emergence of biodegradable or clearable inorganic nanoparticles has made it possible to completely solve this long‐standing conundrum. A comprehensive understanding of the design of these inorganic nanoparticles with their metabolic performance in the body is of crucial importance to advance clinical trials and expand their biological applications in disease diagnosis. Here, a diverse variety of biodegradable or clearable inorganic nanoparticles regarding considerations of the size, morphology, surface chemistry, and doping strategy are highlighted. Their pharmacokinetics, pathways of metabolism in the body, and time required for excretion are discussed. Some inorganic materials intrinsically responsive to various conditions in the tumor microenvironment are also introduced. Finally, an overview of the encountered challenges is provided along with an outlook for applying these inorganic nanoparticles toward future clinical translations.

show abstract

“…[ 63 ] To further enhance Treg numbers, low‐dose rapamycin could be added as an additional bioactive factor, [ 64 ] utilizing recently developed rapamycin‐loaded porous silicon nanoparticles. [ 65,66 ] Moreover, peripheral blood CCR8 + T‐cells were highly enriched with Tregs, and CCL1‐supplemented hydrogel was shown to specifically induce chemotaxis of these cells, providing an insight into the cellular microenvironment in humans upon utilization of CCL‐1‐supplemented hydrogel. Lastly, the findings from Treg encapsulation translated into the 3D bioprinting system as bioprinted Tregs were viable and functional, and protected co‐printed murine islets from xenoresponse mediated by human PBMCs.…”

Section: Resultsmentioning

confidence: 99%

Encapsulation of Human Natural and Induced Regulatory T‐Cells in IL‐2 and CCL1 Supplemented Alginate‐GelMA Hydrogel for 3D Bioprinting

Kim

Hope

Gantumur

et al. 2020

Adv Funct Materials

Self Cite

View full text Add to dashboard Cite

Regulatory T-cells (Tregs) are important modulators of the immune system through their intrinsic suppressive functions. Systemic adoptive transfer of ex vivo expanded Tregs has been extensively investigated for allogeneic transplantation. Due to the time-consuming and costly expansion protocols of Tregs, more targeted approaches could be beneficial. The encapsulation of human natural and induced Tregs for localized immunosuppression is described for the first time. Tregs encapsulated in alginate-gelatin methacryloyl hydrogel remain viable, phenotypically stable, functional, and confined in the structure. Supplementation of the hydrogel with the Treg-specific bioactive factors interleukin-2 and chemokine ligand 1 improves Treg viability, suppressive phenotype, and function, and attracts to the structure CCR8 + T-cells enriched with anti-inflammatory subpopulations, including Tregs, from human peripheral blood. Furthermore, these findings are applicable to 3D bioprinting. Co-axial printing of murine pancreatic islets with human natural and induced Tregs protects the islets from xenoresponse upon co-culture with human peripheral blood mononuclear cells. This establishes the co-encapsulation of Tregs by co-axial 3D bioprinting as a valid option for providing local immune protection to allogeneic cellular transplants such as pancreatic islets.

show abstract

Manipulating human dendritic cell phenotype and function with targeted porous silicon nanoparticles

Cited by 36 publications

References 61 publications

Electrospun Composites of Polycaprolactone and Porous Silicon Nanoparticles for the Tunable Delivery of Small Therapeutic Molecules

Electrospun Composites of Polycaprolactone and Porous Silicon Nanoparticles for the Tunable Delivery of Small Therapeutic Molecules

Degradability and Clearance of Inorganic Nanoparticles for Biomedical Applications

Encapsulation of Human Natural and Induced Regulatory T‐Cells in IL‐2 and CCL1 Supplemented Alginate‐GelMA Hydrogel for 3D Bioprinting

Contact Info

Product

Resources

About