Manipulated Magnetic Nano Particles for Photonic Biomedical Mapping

Abstract

Our main goal is to develop a new speckle based imaging modality for biological applications. The method is based on detection and manipulation of targeted conjugated magnetic nanoparticles (MNPs), which can specifically target cells or other live tissue, and form a concentrated assembly yielding speckle imaging capabilities. The MNPs detection technique employs temporal tracking of secondary speckle patterns while applying a magnetic excitation field that oscillates the particles. We experimentally demonstrate that placing MNPs in an alternating current (AC) electromagnetic field gives rise to modulation of incident light scattered from the material. It was found, via in vitro and two ex vivo experiments, that the resultant modulation spectrum of the speckle patterns is directly associated with the chemical properties such as different sizes, concentration and chemical coating of the MNPs. In ex vivo experiment we demonstrates the potential of the proposed speckle imaging method for detecting the presence of arterial plaque. By taking those results into consideration, the method holds a great promise for non-invasive speckle imaging.

Targeted Magnetic Nanoparticles for Mechanical Lysis of Tumor Cells by Low-Amplitude Alternating Magnetic Field

“…Previous work conducted in our lab demonstrated a gradual decrease in the number of live cells over time, until they reached ~50% of the initial amount by the end of the 5 min AMF treatment [14]. …”

“…The electrical setup used in the present work is the same as previously described [14]. The system includes an electro-magnet that is made of a copper coil surrounding an EC Ferrite core.…”

“…To specifically target the EGF receptor, we added C225 (Cetuximab, Merck KGaA, Darm-stadt, Germany), conjugated by a covalent bonding of an amine (NH 2 ) group to the carboxylic group of SH–PEG–COOH. We used MNPs sized 50, 100, and 200 nm due to the simplicity of their synthesis and based on our previous study [ 14 ]. The classification of materials with magnetic properties is based on their magnetic susceptibility (χ), which is defined by the ratio of the induced magnetization (M) to the applied magnetic field (H).…”

“…AMFs were produced by an electrical system that includes an electro-magnet made of copper coil surrounding an EC Ferrite core, with 30 Ω resistance, connected to a waveform generator that sets the voltage and frequencies provided to the coil. This system was used in our previous study [ 14 ]. The electrical parameters used were a 4 V, 4 Hz unipolar magnetic field.…”

Advances in imaging strategies for in vivo tracking of exosomes

“…Tracking of exosomes using magnetic imaging MRI has advantages such as being radiation-free and having high spatial resolution (Crabbe et al, 2010). In order to be detectable by MRI, exosomes must be labeled with magnetic contrast agents, such as superparamagnetic iron oxide nanoparticles (SPIONs; Busato et al, 2017;Motiei et al, 2019;Vegerhof et al, 2015). Hu et al demonstrated for the first time that exosomes loaded with 5 nm SPIONs cargo can be imaged and tracked using the MRI modality in vivo.…”