Mangrove-Associated Fungal Communities Are Differentiated by Geographic Location and Host Structure

Lee, Nicole Li Ying; Huang, Danwei; Quek, Zheng Bin Randolph; Lee, Jen Nie; Wainwright, Benjamin J.

doi:10.3389/fmicb.2019.02456

Cited by 43 publications

(35 citation statements)

References 79 publications

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“…Shannon diversity values are comparable among all locations when all organs and sediment are combined, and fungal community diversity in the leaves, fruits and pneumatophores is comparable, while sediment samples are approximately twice as diverse as all other samples. This is consistent with previous work showing that soils are highly diverse reservoirs of fungal biodiversity, containing hundreds of thousands of fungal species (Bridge and Spooner 2001 ; Lee et al 2019a ). Likewise we find the highest number of fungal ASVs in the sediment samples.…”

Section: Discussionsupporting

confidence: 93%

“…As in Tisthammer et al ( 2016 ), and also shown for another mangrove Avicenna alba recently (Lee et al 2019a ), we suggest that the fungal community distribution is strongly shaped by the environment, a consequence of differences in habitat type throughout Peninsular Malaysia and Singapore. Further supporting this idea, the coasts of Malay Peninsula are split in two by the Titiwangsa Mountain range, which forms the backbone of the peninsula with its > 2000-m maximum elevation.…”

Section: Discussionsupporting

confidence: 86%

“…Pioneering mycological research documented fungi on mangrove roots (Cribb and Cribb 1955 ) and described mangrove-associated fungi throughout the world (Kohlmeyer 1969 ). New species continue to be described today, and examination of various plant parts and geographic localities are finding unexpected diversity and strong community structuring of fungi in mangroves (Kumar et al 2019 ; Lee et al 2019a ). In particular, mangroves abut marine and terrestrial environments, offering a unique opportunity to study plants that potentially contain obligate marine fungi, terrestrial fungi and those that can survive, or at least tolerate both environments to some degree.…”

Section: Introductionmentioning

confidence: 99%

“…Consequently, our understanding of fungal biogeography in marine environments remains rudimentary. However, the research that has been performed suggests that the environment strongly influences spatial patterns of marine fungal communities (Tisthammer et al 2016 ; Lee et al 2019a ). Environmental and habitat differences also explain differences in seagrass and macroalgal associated fungal communities in Southeast Asia (Wainwright et al 2019b , c ).…”

Section: Introductionmentioning

confidence: 99%

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Distinct fungal communities associated with different organs of the mangrove Sonneratia alba in the Malay Peninsula

et al. 2020

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Mangrove forests are key tropical marine ecosystems that are rich in fungi, but our understanding of fungal communities associated with mangrove trees and their various organs remains limited because much of the diversity lies within the microbiome. In this study, we investigated the fungal communities associated with the mangrove tree Sonneratia alba throughout Peninsular Malaysia and Singapore. At each sampling location, we collected leaves, fruits, pneumatophores and sediment samples and performed amplicon sequencing of the ribosomal internal transcribed spacer 1 to characterise the associated communities. Results show distinct fungal communities at each sampled location with further differentiation according to the plant part. We find a significant distance decay of similarity, particularly for sediment samples due to the greater variability of sediment environments relative to the more stable fungal habitats provided by living plant organs. We are able to assign taxonomy to the majority of sequences from leaves and fruits, but a much larger portion of the sequences recovered from pneumatophores and sediment samples could not be identified. This pattern underscores the limited mycological research performed in marine environments and demonstrates the need for a concerted research effort on multiple species to fully characterise the coastal microbiome and its role in the functioning of marine ecosystems.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Distinct fungal communities associated with different organs of the mangrove Sonneratia alba in the Malay Peninsula

et al. 2020

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“…Like other studies examining microbial diversity and coral-associated bacteria throughout Singapore and the region, we show significant differences in microbial community structure among sampling locations 10,24,39,40 . All samples are generally dominated by Proteobacteria and Cyanobacteria, and samples collected from Tanah Merah appear most distinct in comparison to other sites.…”

Section: Discussionsupporting

confidence: 71%

Host age is not a consistent predictor of microbial diversity in the coral Porites lutea

Wainwright

Zahn

Afiq‐Rosli

et al. 2020

Sci Rep

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Corals harbour diverse microbial communities that can change in composition as the host grows in age and size. Larger and older colonies have been shown to host a higher diversity of microbial taxa and this has been suggested to be a consequence of their more numerous, complex and varied micro-niches available. However, the effects of host age on community structure and diversity of microbial associates remain equivocal in the few studies performed to date. To test this relationship more robustly, we use established techniques to accurately determine coral host age by quantifying annual skeletal banding patterns, and utilise high-throughput sequencing to comprehensively characterise the microbiome of the common reef-building coral, Porites lutea. Our results indicate no clear link between coral age and microbial diversity or richness. Different sites display distinct age-dependent diversity patterns, with more anthropogenically impacted reefs appearing to show a winnowing of microbial diversity with host age, possibly a consequence of corals adapting to degraded environments. Less impacted sites do not show a signature of winnowing, and we observe increases in microbial richness and diversity as the host ages. Furthermore, we demonstrate that corals of a similar age from the same reef can show very different microbial richness and diversity. Corals contain diverse and complex microbial communities that play critical roles in maintaining and promoting host fitness and survival 1-3. They are involved in nutrient cycling 4 , and help prevent colonization by pathogenic microbes through the occupation of potential niches 5 and the production of specific antibacterial compounds 2. These communities can differ over space and time, among host species, and with environmental perturbations 6-9. Yet, some coral-associated bacterial communities remain constant with no discernible temporal changes 10,11. These contrasting patterns demonstrate some of the challenges faced when attempting to understand the coral microbiome. Numerous studies show that host-associated microbes can and do differ over comparatively small spatial scales 7,12-14. However, few have explicitly examined how host age affects the microbial community, and those that have are limited by colony size as a proxy to obtain a crude estimate of age 15,16. These studies show conflicting patterns with one reporting a steady, yet significant decrease in bacterial diversity with increasing size 16 , while the other demonstrates a general stepwise increase in bacterial diversity with increasing size followed by a slight decrease in diversity of the largest and assumed oldest corals 15. However, it should be noted that the latter study by Williams et al. 15 is limited by the young age of the assumed oldest corals, which have been estimated at only 10-12 years old. Pollock et al. 16 suggest that early coral recruits and larvae have more diverse microbiomes in comparison to later life stages, which is consistent with previous work suggesting that a 'winnowing' of the mic...

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Evidence for the genetic similarity rule at an expanding mangrove range limit

Kennedy

Antwis

Preziosi

et al. 2021

American J of Botany

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Host-plant genetic variation can shape associated communities of organisms. These community-genetic effects include (1) genetically similar hosts harboring similar associated communities (i.e., the genetic similarity rule) and (2) host-plant heterozygosity increasing associated community diversity. Community-genetic effects are predicted to be less prominent in plant systems with limited genetic variation, such as those at distributional range limits. Yet, empirical evidence from such systems is limited. Methods: We sampled a natural population of a mangrove foundation species (Avicennia germinans) at an expanding range limit in Florida, USA. We measured genetic variation within and among 40 host trees with 24 nuclear microsatellite loci and characterized their foliar endophytic fungal communities with internal transcribed spacer (ITS1) gene amplicon sequencing. We evaluated relationships among host-tree genetic variation, host-tree spatial location, and the associated fungal communities. Results: Genetic diversity was low across all host trees (mean: 2.6 alleles per locus) and associated fungal communities were relatively homogeneous (five sequence variants represented 78% of all reads). We found (1) genetically similar host trees harbored similar fungal communities, with no detectable effect of interhost geographic distance. (2) Host-tree heterozygosity had no detectable effect, while host-tree absolute spatial location affected community alpha diversity. Conclusions: This research supports the genetic similarity rule within a range limit population and helps broaden the current scope of community genetics theory by demonstrating that community-genetic effects can occur even at expanding distributional limits where host-plant genetic variation may be limited. Our findings also provide the first documentation of community-genetic effects in a natural mangrove system.

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Mangrove-Associated Fungal Communities Are Differentiated by Geographic Location and Host Structure

Cited by 43 publications

References 79 publications

Distinct fungal communities associated with different organs of the mangrove Sonneratia alba in the Malay Peninsula

Distinct fungal communities associated with different organs of the mangrove Sonneratia alba in the Malay Peninsula

Host age is not a consistent predictor of microbial diversity in the coral Porites lutea

Evidence for the genetic similarity rule at an expanding mangrove range limit

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