Mangrove algae as sustainable microbial cell factory for cellulosic biomass degradation and lipid production

Abstract

Human civilization faces a huge energy crisis due to climate alterations and reduction in fossil fuel reserves. Biofuels are sustainable platform that can combat this burning issue currently. Biodiesel is a potential fossil fuel alternative from cooking, soybean, or plant-derived oil resources. Existing biodiesel production processes are directly competing with food chain. Algae are impressive alternatives for biodiesel generation as algae grow without competition with food chain, have an inherently faster growth rate, and have higher lipid content under diverse nutrient-limiting environments. Lignocellulosic waste (LCW) accumulation has been increasing through anthropogenic activities. Accumulation of LCW causes ill effects on environment and human health. Thus, a faster paradigm shift needs to be required for establishing a single platform to perform lignocellulosic biomass degradation along with higher lipid production as biodiesel precursor. Few studies show algal endoglucanase or cellulase activities for degrading LCW to generate monomeric constituents which can further be channelized to produce higher biomass and lipid contents as biodiesel precursor. Thus, the present study focuses on a feasibility attempt to show that mangrove algal isolates could be a potential microbial cell factory for cellulose degradation as a major constituent of LCW and higher lipid production as potential precursor for biodiesel production in near future.

Isolation and biochemical characterization of natural algal isolates from Kangsabati River, West Bengal toward single-cell proteins production

“…Algal isolates were individually inoculated in sterilized 50 mL falcon tubes with 25 mL of sterilized Bold's basal medium (BBM) in the laboratory after filtering and serial dilution (10 -8 times). To prepare 200 mL BBM, the following key nutrients were required: 5g.-NaNO [13,14]. To maintain the purity of the microalgae cultures, repeated streaking on an agar plate was done routinely.…”