Mangiferin-Enriched Mn–Hydroxyapatite Coupled with β-TCP Scaffolds Simultaneously Exhibit Osteogenicity and Anti-Bacterial Efficacy

Abstract

Biphasic calcium phosphate (BCP) containing β-tricalcium phosphate and manganese (Mn)-substituted hydroxyapatite (HAP) was synthesized. Biomedical scaffolds were prepared using this synthesized powder on a sacrificial polyurethane sponge template after the incorporation of mangiferin (MAN). Mn was substituted at a concentration of 5% and 10% in HAP to examine the efficacy of Mn at various concentrations. The phase analysis of the as-formed BCP scaffold was carried out by X-ray diffraction analysis, while the qualitative observation of morphology and the osteoblast cell differentiation were carried out by scanning electron microscopy and confocal laser scanning microscopy techniques. Gene expressions of osteocalcin, collagen 1, and RUNX2 were carried out using qRT-PCR analyses. Significantly higher (p < 0.05) levels of ALP activity were observed with extended osteoblast induction on the mangiferin-incorporated BCP scaffolds. After characterization of the specimens, it was found that the scaffolds with 10% Mn-incorporated BCP with mangiferin showed better osteogenicity and simultaneously the same scaffolds exhibited higher anti-bacterial properties as observed from the bacterial viability test. This study was carried out to evaluate the efficacy of Mn and MAN in BCP for osteogenicity and antibacterial action.

CpG ODN/Mangiferin Dual Delivery through Calcium Alginate Hydrogels Inhibits Immune-Mediated Osteoclastogenesis and Promotes Alveolar Bone Regeneration in Mice

“…This is consistent with the reports by others showing that mangiferin positively regulates osteoblast differentiation and suppresses osteoclastogenesis, bone resorption, and RANKL-induced activation of NF-κB and ERK [24,61]. It has been shown that MAG promotes the osteogenic differentiation of stem cells [62,63] and promotes the regeneration of alveolar bone defects [64,65]. Another study showed that CpG ODN increased the early ALP activity of mesenchymal stem cells fourfold without affecting osteoclast formation [66].…”

On the Ion Implantation Synthesis of Ag-Embedded Over Sr-Substituted Hydroxyapatite on a Nano-Topography Patterned Ti for Application in Acetabular Fracture Sites

“…The cultured media was replaced with fresh media to eliminate non-adherent cells on every alternative day for two weeks. 36 Live/Dead Osteoblast Cell Analyses…”

Magnesium-Substituted Hydroxyapatite /Spider Silk fibroin /N-Carboxymethyl Chitosan Based Microsphere: A potential template for bone regeneration

“…Prolonged exposure to extracellular uid may result in bio lm formation, especially S. aureus strain on the microsphere surfaces. MTT assay test was performed to check the viability of S. aureus bacterial cells, on both polarized and non-polarized MgHA-sSilk-NCMCh microspheres [21].…”