Manganese superoxide dismutase, glutathione peroxidase and catalase gene polymorphisms and clinical outcomes in acute kidney injury

Abstract

Introduction The aim of this study was to evaluate the potential association of single gene polymorphisms of manganese superoxide dismutase (MnSOD), glutathione peroxidase 1 (GPX1) and catalase (CAT) with clinical outcomes of acute kidney injury (AKI). Materials and methods Ninety AKI patients and 101 healthy volunteers were included in the study. Determination of MnSOD rs4880, GPX1 rs1050450 and CAT rs769217 polymorphisms was performed using real-time polymerase chain reaction amplification. The duration of hospitalization of AKI patients, dialysis and intensive care requirements, sepsis, oliguria and in-hospital mortality rates were assessed. Results The MnSOD, GPX1 and CAT genotypes and allele frequencies of AKI patients did not differ significantly from those of healthy controls. In patients with a T allele in the ninth exon of the CAT gene, intensive care requirements were greater than those of patients with the CC genotype (p ¼ 0.04). In addition, sepsis and in-hospital mortality were observed significantly more frequently in patients with a T allele in the ninth exon of the CAT gene (p ¼ 0.03). Logistic regression analysis determined that bearing a T allele was the primary determinant of intensive care requirements and in-hospital mortality, independent of patient age, gender, presence of diabetes and dialysis requirements (OR 6.10, 95% CI 1.34-27.81, p ¼ 0.02 and OR 10.25, 95% CI 1.13-92.80, p ¼ 0.04, respectively). Conclusion Among AKI patients in the Turkish population, hospital morbidity and mortality were found to be more frequent in patients bearing a T allele of the rs769217 polymorphism of the CAT gene.

Visit to intensive care of 2050

“…Triage is performed by this computerized system, with patients being admitted to the ICU only if data indicate that they will respond to treatment: if their telomeres demonstrate a tendency for longevity [3], their genetic testing demonstrates that they are not likely to develop non-iatrogenic complications; e.g. ARDS [4] sepsis [5], renal failure [6]) and their streaming merged physiological data trend suggest a good likelihood of response to therapy [7].…”

Genetic Susceptibility to Acute Kidney Injury

“…The balance between the formation of reactive oxygen species and antioxidants is determinant. The T allele of the rs769217 variant of the antioxidant defense enzyme catalase (CAT) gene was associated with hospital morbidity and death among AKI patients in a Turkish population [141]. BCL2 has a major part in apoptosis signaling as an antiapoptosis protein [142].…”

Genetic Polymorphisms of MnSOD Modify the Impacts of Environmental Melamine on Oxidative Stress and Early Kidney Injury in Calcium Urolithiasis Patients

“…MnSOD can decompose toxic ROS, superoxide anions, to hydrogen peroxide, which is then converted to non-toxic water and oxygen by GPX1 and CAT in the mitochondria [ 18 ]. Several single-nucleotide polymorphisms (SNPs) of those antioxidant enzyme genes have been associated with various diseases [ 19 ], including kidney diseases [ 20 , 21 , 22 ].…”

“…Since environmental toxicant-associated kidney damage might be influenced by genetic factors [ 23 ], it is possible that the SNPs of antioxidant enzyme genes (e.g., MnSOD , GPX1 , CAT ) could modify the effects of environmental melamine exposure on the risk of oxidative stress and renal tubular injury in humans. To find out, we selected five candidate SNPs of antioxidant enzyme genes ( MnSOD: rs4880 and rs5746136, GPX1: rs1800668, CAT: rs1001179 and rs769217), most of which have been linked to kidney diseases [ 20 , 21 , 22 ], to study each of their effects when combined with environmental melamine exposure on 8-OHdG and MDA, two biomarkers of oxidative stress, and NAG, a biomarker of renal tubular injury, in urine.…”