Manganese Peroxidases: Molecular Diversity, Heterologous Expression, and Applications

Saroj, Samta; Agarwal, Pragati; Dubey, Swati; Singh, Rajesh

doi:10.1007/978-81-322-1094-8_6

Cited by 5 publications

(1 citation statement)

References 118 publications

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“…The oxidative potential of MnP has been key in its ability to oxidize polycyclic aromatic hydro-carbons, chlorophenols, industrial effluents (mainly from the pulp and paper industry), and the bioremediation of contaminated soils (Saroj et al 2013). The catalytic cycle of this enzyme is similar to that of other peroxidases, since it involves a two-electron oxidation.…”

Section: Introductionmentioning

confidence: 99%

Bundling the removal of emerging contaminants with the production of ligninolytic enzymes from residual streams

González-Rodríguez

Lú-Chau

Trueba-Santiso

et al. 2022

Appl Microbiol Biotechnol

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Enzymes offer interesting features as biological catalysts for industry: high specificity, activity under mild conditions, accessibility, and environmental friendliness. Being able to produce enzymes in large quantities and having them available in a stable and reusable form reduces the production costs of any enzyme-based process. Agricultural residues have recently demonstrated their potential as substrates to produce ligninolytic enzymes by different white rot fungi. In this study, the biotechnological production of a manganese peroxidase (MnP) by Irpex lacteus was conducted through solid-state fermentation (SSF) with wheat straw as substrate and submerged fermentation (SmF) employing wheat straw extract (WSE). The obtained enzyme cocktail also showed manganese-independent activity (MiP), related to the presence of a short MnP and a dye-decolorizing peroxidase (DyP) which was confirmed by shotgun proteomic analyses. In view of the enhanced production of ligninolytic enzymes in SmF, different parameters such as WSE concentration and nitrogen source were evaluated. The highest enzyme titers were obtained with a medium formulated with glucose and peptone (339 U/L MnP and 15 U/L MiP). The scale-up to a 30 L reactor achieved similar activities, demonstrating the feasibility of enzyme production from the residual substrate at different production scales. Degradation of five emerging pollutants was performed to demonstrate the high oxidative capacity of the enzyme. Complete removal of hormones and bisphenol A was achieved in less than 1 h, whereas almost 30% degradation of carbamazepine was achieved in 24 h, which is a significant improvement compared to previous enzymatic treatments of this compound. Key points • Wheat straw extract is suitable for the growth of I. lacteus. • The enzyme cocktail obtained allows the degradation of emerging contaminants. • Mn-dependent and Mn-independent activities increases the catalytic potential. Graphical abstract

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Section: Introductionmentioning

confidence: 99%

Bundling the removal of emerging contaminants with the production of ligninolytic enzymes from residual streams

González-Rodríguez

Lú-Chau

Trueba-Santiso

et al. 2022

Appl Microbiol Biotechnol

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Differential expression of peroxidase and ABC transporter as the key regulatory components for degradation of azo dyes by Penicillium oxalicum SAR-3

Saroj

Kumar

Prasad

et al. 2014

Funct Integr Genomics

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Fungal species are potential dye decomposers since these secrete spectra of extracellular enzymes involved in catabolism. However, cellular mechanisms underlying azo dye catalysis and detoxification are incompletely understood and obscure. A potential strain designated as Penicillium oxalicum SAR-3 demonstrated broad-spectrum catabolic ability of different azo dyes. A forward suppression subtractive hybridization (SSH) cDNA library of P. oxalicum SAR-3 constructed in presence and absence of azo dye Acid Red 183 resulted in identification of 183 unique expressed sequence tags (ESTs) which were functionally classified into 12 functional categories. A number of novel genes that affect specifically organic azo dye degradation were discovered. Although the ABC transporters and peroxidases emerged as prominent hot spot for azo dye detoxification, we also identified a number of proteins that are more proximally related to stress-responsive gene expression. Majority of the ESTs (29.5%) were grouped as hypothetical/unknown indicating the presence of putatively novel genes. Analysis of few ESTs through quantitative real-time reverse transcription polymerase chain reaction revealed their possible role in AR183 degradation. The ESTs identified in the SSH library provide a novel insight on the transcripts that are expressed in P. oxalicum strain SAR-3 in response to AR183.

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Production of high-value oxidative enzymes by Cyathus bulleri on agricultural and agri-food wastes for application in the textile sector

Afreen,

Mishra

2023

World J Microbiol Biotechnol

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Manganese Peroxidases: Molecular Diversity, Heterologous Expression, and Applications

Cited by 5 publications

References 118 publications

Bundling the removal of emerging contaminants with the production of ligninolytic enzymes from residual streams

Bundling the removal of emerging contaminants with the production of ligninolytic enzymes from residual streams

Differential expression of peroxidase and ABC transporter as the key regulatory components for degradation of azo dyes by Penicillium oxalicum SAR-3

Production of high-value oxidative enzymes by Cyathus bulleri on agricultural and agri-food wastes for application in the textile sector

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