Manganese (Mn2+) tolerance and biosorption by Meyerozyma guilliermondii and Meyerozyma caribbica strains

Amorim, Soraya Sander; Ruas, France Anne Dias; Barboza, Natália Rocha; Neves, Viviano Gomes de Oliveira; Leão, Versiane Albis; Guerra‐Sá, Renata

doi:10.1016/j.jece.2018.06.061

Cited by 14 publications

(11 citation statements)

References 36 publications

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“…In a previous publication, we demonstrated that this strain of yeast may be employed in bioremediation processes to remove Mn 2+ ions from water contaminated by mines. We confirmed this biosorptive removal capability using kinetic equations (Amorim et al, 2018), and recently described the first soluble proteome for this genus and this particular species (Ruas et al, 2019).…”

Section: Introductionsupporting

confidence: 78%

“…In a previous study, we isolated M. guilliermondii and demonstrated that this species of yeast removes Mn 2+ ions efficiently. Indeed, our experiments indicated that 100% of Mn 2+ ions were removed from water in only 6 days (0.91 mM Mn 2+ ); the removal kinetics were also indicative of efficient bioremediation (Amorim et al, 2018). Species that are capable of tolerating excessive concentrations of heavy metals may possess specific biochemical and molecular characteristics such as the expression of specific proteins and metabolic pathways that confer the ability to survive under heavy metal stress.…”

Section: Discussionmentioning

confidence: 76%

“…Due to these characteristics, this yeast can be effectively used for bioremediation of metal contamination in contaminated water. Results from the present study provide novel insights into molecular and biochemical mechanisms that allow this species of yeast to tolerate excessive concentrations of Mn 2+ (Eccles, 1999;Shakya et al, 2015;Amorim et al, 2018).…”

Section: Discussionmentioning

confidence: 88%

“…These binders are predominant in metal-binding sites, when dead biomass is used as a biosorbent material (Kotrba and Ruml, 2000;Farooq et al, 2010). Indeed, a recent study involving M. guilliermondii reported that dead biomass removed manganese ions more efficiently than live biomass (Amorim et al, 2018), suggesting that metal binding compounds may play a role in this process.…”

Section: Discussionmentioning

confidence: 99%

“…The development of new ecological strategies for the efficient treatment of contaminated water is therefore a key target for researchers. Such strategies should be based on biological removal, or bioremediation, which is safer and more economically viable than traditional methods (Azubuike et al, 2016;Bahafid et al, 2017;Amorim et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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In silico Prediction of Protein–Protein Interaction Network Induced by Manganese II in Meyerozyma guilliermondii

Guerra‐Sá

2020

Front. Microbiol.

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Recently, there has been an increasing interest in the use of yeast to produce biosorbent materials, because yeast is economical to use, adaptable to a variety of conditions, and amenable to morphological manipulations to yield better raw biomaterials. Previous studies from our laboratory have shown that Meyerozyma guilliermondii, a nonpathogenic haploid yeast (ascomycete), exhibits excellent biosorption capacity for Mn 2+ , as demonstrated by kinetic analyses. Shotgun/bottom-up analyses of soluble fractions revealed a total of 1257 identified molecules, with 117 proteins expressed in the absence of Mn 2+ and 69 expressed only in the presence of Mn 2+ . In this article, we describe the first in silico prediction and screening of protein-protein interactions (PPIs) in M. guilliermondii using experimental data from shotgun/bottom-up analyses. We also present the categorization of biological processes (BPs), molecular functions (MFs), and metabolic pathways of 71 proteins upregulated in the M. guilliermondii proteome in response to stress caused by an excess of Mn 2+ ions. Most of the annotated proteins were related to oxidation-reduction processes, metabolism, and response to oxidative stress. We identified seven functional enrichments and 42 metabolic pathways; most proteins belonged to pathways related to metabolic pathways (19 proteins) followed by the biosynthesis of secondary metabolites (10 proteins) in the presence of Mn 2+ . Using our data, it is possible to infer that defense mechanisms minimize the impact of Mn 2+ via the expression of antioxidant proteins, thus allowing adjustment during the defense response. Previous studies have not considered protein interactions in this genus in a manner that permits comparisons. Consequently, the findings of the current study are innovative, highly relevant, and provide a description of interactive complexes and networks that yield insight into the cellular processes of M. guilliermondii. Collectively, our data will allow researchers to explore the biotechnological potential of M. guilliermondii in future bioremediation processes.

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Section: Introductionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 76%