Mandibular Segmental Defect Regenerated With Macroporous Biphasic Calcium Phosphate, Collagen Membrane, and Bone Marrow Graft in Dogs

Abstract

This model succeeded in regenerating a large segmental defect in the mandible. An investigation with a postimplantation radiation delivery schedule is required with the use of this model, which should be considered as a preclinical study for a bone tissue engineering approach in patients with cancer-related bone defects.

Tissue-Engineered Mandibular Bone Reconstruction for Continuity Defects: A Systematic Approach to the Literature

“…15,18,29,99,[101][102][103]109 In several studies, teeth were extracted in advance, and oral mucosa was allowed to heal completely before resective surgery and reconstructive therapy. 4,26,105,106 Beside the diversity in animal models, study design as well as healing periods after reconstructive surgery (range: 4-48 weeks) were not uniform. The follow-up periods were related to differences in animal species; for example, dog (12-48 weeks), monkey (16-30 weeks), sheep (12-20 weeks), rabbit (12-16 weeks), goat (6-16 weeks), and minipigs (16 weeks).…”

“…In summary, autogenous bone precursor cells or autogenous osteogenic tissues were primarily combined with calcium phosphate ceramic scaffolds, such as beta-tri calcium phosphate (b-TCP) 4,11,25,26,106 and biphasic calcium phosphate ceramic, 26 or pyrolized bovine bone 100 or calcium carbonate, such as natural corals. 10,105 Considering the primary outcome variable bone bridging 4,11,18,26,[104][105][106][107]117 as well as the co-outcome variables bone ingrowth 100 and biomechanical testing, 4,10,25 autogenous bone precursor cells or autogenous tissues seeded onto calcium phosphate ceramic scaffolds showed in preclinical animal studies the potential of an alternative to autograft bone for mandibular bone reconstruction in continuity defects.…”

“…10,105 Considering the primary outcome variable bone bridging 4,11,18,26,[104][105][106][107]117 as well as the co-outcome variables bone ingrowth 100 and biomechanical testing, 4,10,25 autogenous bone precursor cells or autogenous tissues seeded onto calcium phosphate ceramic scaffolds showed in preclinical animal studies the potential of an alternative to autograft bone for mandibular bone reconstruction in continuity defects. Moreover, autogenous bone precursor cells or autogenous osteogenic tissues seeded onto or mixed with collagen sponges 18,108 demonstrated in preclinical animal studies promising results in terms of the primary outcome variable bone bridging 18 or the co-outcome variable bone ingrowths.…”

“…Furthermore, these scaffolds should possess (3) suitable surface chemistry for cell attachment, proliferation, and differentiation, and (4) mechanical properties to match those of the tissues at the site of implantation. 9 At present, a multitude of scaffolds made of various material [10][11][12][13][14][15][16][17][18][19] in combination with bioactive substances or osteogenic bone marrow stromal cells (BMSCs) 10,[20][21][22][23][24][25][26] to initiate or enhance bone formation [15][16][17]19,[27][28][29][30][31][32][33] are under study. In 2006, Ikada defined a concept on methodology in tissue engineering as (1) placing the construct scaffold in a bioreactor to reconstruct an engineered tissue in vitro called in vitro (or ex vivo) tissue engineering and (2) implantation of the construct scaffold in the body until a new tissue is regenerated in vivo called in vivo (or in situ) tissue engineering.…”

Method of maxillofacial reconstruction using individualized implants made of bioactive ceramics

“…Из керамических материалов, предназначенных для реконструкции и замещения костной ткани, био-активные и резорбируемые вызывают наибольший интерес. Они воспринимаются организмом не как чу-жеродные, и на границе с костью биохимические ре-акции способствуют интенсивному прорастанию тка-ней в имплантат и активному остеогенезу [8][9][10].…”