Mandelate Racemase and Mandelate Dehydrogenase Coexpressed RecombinantEscherichia coliin the Synthesis of Benzoylformate

Biosynthesis of Phenylglyoxylic Acid by LhDMDH, a Novel d-Mandelate Dehydrogenase with High Catalytic Activity

“…The specific oxidation activity of purified LhDMDH was 1200 U•mg −1 , which was close to four times that of the probe 7 and significantly higher than the other reported Dmandelate dehydrogenases. 3,6,22 The specific oxidation activity of LcDMDH, a D-mandelate dehydrogenase cloned from Leuconostoc citreum, was 40.6 U•mg −1 . On the other hand, the specific oxidation activity of LlDMDH-2, a D-mandelate dehydrogenase cloned from Lactococcus lactis, was 11.7 U• mg −1 .…”

“…Phenylglyoxylic acid (PGA) plays an important role as a building block in the field of chemical synthesis and is widely used to synthesize pharmaceutical intermediates or food additives. 1,2 Compared with chemical preparation via dehydrogenation of mandelic acid, hydrolysis of benzoyl cyanide, or oxidation of styrene, 2,3 the microbial synthesis of PGA from mandelic acid provides an ecofriendly approach to industrialscale production, 2,4 which addresses concerns about the environmental aspects of chemical manufacturing. Enantioselective conversion of D-mandelic acid to PGA has been reported in Alcaligenes bronchisepticus, Pseudomonas polycolor, Gibberella f ujikuroi, and Pseudomonas putida.…”

“…5,6 D-Mandelate dehydrogenase (DMDH), a member of the ketopantoate reductase family, has much potential for use in the conversion D-mandelic acid to PGA. 3,6,7 It also has tremendous potential in the biosynthesis of enantiopure mandelic acids, which are also important intermediates for synthesis of pharmaceutical and natural products. 8,9 The heterologous expression of DMDH genes from Lactobacillus brevis, 7 Pseudomonas putida, 6 and Pseudomonas aeruginosa 3 in Escherichia coli produced enzyme capable of oxidizing mandelic acid into PGA.…”

“…3,6,7 It also has tremendous potential in the biosynthesis of enantiopure mandelic acids, which are also important intermediates for synthesis of pharmaceutical and natural products. 8,9 The heterologous expression of DMDH genes from Lactobacillus brevis, 7 Pseudomonas putida, 6 and Pseudomonas aeruginosa 3 in Escherichia coli produced enzyme capable of oxidizing mandelic acid into PGA. However, the poor activity of these recombinant mandelate dehydrogenases impedes their industrial application.…”

One-Pot Synthesis of Phenylglyoxylic Acid from Racemic Mandelic Acids via Cascade Biocatalysis

“…Phenylglyoxylic acid (PGA) are key building blocks in the chemical synthesis process and widely used to synthesize pharmaceutical intermediates or food additives. − Compared with chemical preparation generally involve toxic cyanide and complex processes, , microbial or enzymatic biosynthesis of PGA provides an eco-friendly approach to industrial-scale production. ,, It is reported that many microorganisms can selectively transform mandelic acid to PGA, including Gibberella fujikuroi, Alcaligenes bronchisepticus, Pseudomonas polycolor, and Pseudomonas putida. , d -Mandelate dehydrogenase (DMDH) plays a key role in the process of converting d -mandelic acid to PGA, and it is a member of ketopantoate reductase family. ,, Heterologous expression of DMDH genes from many microorganisms in Escherichia coli was capable to oxidize mandelic acid into PGA. , In our previous study, the PGA yield was about 40% from 50 mM racemic mandelic acid catalyzed by coculturing two engineered E. coli strains .…”