The harmful, filamentous cyanobacteria Microseira
(Lyngbya) wollei produces several toxic analogues
of saxitoxin (Lyngbya wollei toxins
1–6, or LWTs 1–6), grows in shallow water, and can deposit
significant biomass on nearby shorelines. Here, we show that the LWTs
are stable in the biomass during subsequent drying but that the process
facilitates the later release of LWTs upon return to the water column.
Under basic conditions, LWTs hydrolyzed to generate products that
were significantly more neurotoxic than the initial toxins. Aqueous
LWTs were subjected to conditions of covarying temperature and pH,
and their degradation rates and products were determined at each condition.
LWTs 1, 5, and 6 degraded faster at pH ≥ 8 at all temperatures.
Their degradation products, which included decarbamoyl saxitoxin and
LWT 4, were consistent with a base-catalyzed hydrolysis mechanism
and represented a net increase in total biomass toxicity normalized
against the equivalent toxicity of saxitoxin. The corresponding pre-exponential
terms and activation energies for hydrolysis were obtained for pH
6–10 over the temperature range 10–40 °C. A locally
weighted scatterplot smoothing (LOWESS) regression was developed to
predict the loss of parent toxins and subsequent products in the water
column under conditions corresponding to those commonly encountered
in cyanobacterial blooms.