Management of seed-borne fungi in cowpea using leaf extracts and sodium bicarbonate

Kareem, Kehinde Titilope; Afolabi, Qasim Olaitan; Shorinmade, A.Y.; Akinbode, O. A.

doi:10.4314/jasem.v22i4.23

Cited by 2 publications

(2 citation statements)

References 15 publications

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“…Sodium hydrogen carbonate and leaf extracts of Carica papaya showed good inhibiting power against Aspergillus niger and Botryodiplodia theobromae in soybean seeds . Effectiveness of sodium hydrogen carbonate was the object of another study conducted by Kareem et al (2018). Sodium hydrogen carbonate had broad-spectrum activities on tested fungal species (Aspergillus niger, Candida spp., Rhizopus stolonifer, Pythium spp., Trichoderma spp.)…”

Section: Influence Of Chemical Agents On the Reduction Of The Number Of Microorganismsmentioning

confidence: 99%

“…In the food industry, sodium hypochlorite (NaOCl) is the most commonly used disinfectant, due to its broad spectrum of action, rapid antibacterial action, but also due to the ease of application and low cost (Fukuzaki, 2006). The use of sodium hydrogen carbonate (NaHCO 3 ) in the food industry is more related to its sensory properties, although its antimicrobial power has been proven (Curran & Montville, 1989;Kareem et al, 2018). The advantages of using sodium hydrogen carbonate as a disinfectant are its safety, simplicity of use, and low costs.…”

Section: Introductionmentioning

confidence: 99%

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Influence of different chemical agents and storage conditions on the microbiological content of industrial hemp (Cannabis sativa L.) seeds

Đerić¹,

Todorić²,

Rošul³

et al. 2020

Food & Feed Res

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This study aimed to test different chemical agents to obtain microbiologically safe industrial hemp seeds that could be used for further food processing (with the reduced total number of microorganisms, total number of moulds and yeasts, and total number of Enterobacteriaceae). In order to obtain seeds applicable for food consumption, optimal storage temperature conditions (room temperature, refrigerator, freezer), method of seed packaging (vacuum/without vacuum), and the application of various chemical treatments (ethanol, sodium hydrogen carbonate, sodium hypochlorite) were tested on the certified industrial hemp seeds, produced in two consecutive years. Optimal storage conditions differed for different microorganisms, and the most optimal storage was at room temperature, for seeds produced in 2018, in the treatment to reduce the total number of Enterobacteriaceae and the total number of microorganisms. When storing seeds from 2018 in order to reduce the number of yeasts and moulds, a slightly lower number was spotted when seeds were stored in a vacuum-sealed bag, at the refrigerator/freezer temperature. For hemp seeds produced in 2019, the most optimal storage conditions were at the refrigerator (for reduction of the total number of Enterobacteriaceae) and freezer temperature (for reduction of the total number of microorganisms). For the reduction of the total number of moulds and yeasts, optimal conditions were at room temperature. Ethanol (75%, v/v) was the most effective disinfectant among the tested chemicals regardless of the initial number of microorganisms, with log reduction of 3.2 (for the total number of Enterobacteriaceae), 2.9 log (for the total number of microorganisms), and total reduction of the total number of yeasts and moulds after 10 minutes, for the seeds harvested in 2019, which were far more contaminated than the seeds harvested in 2018. Considering the price of the disinfection method with ethanol, sodium hypochlorite may be a better solution for the reduction of the number of microbiota on the seeds.

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Section: Influence Of Chemical Agents On the Reduction Of The Number Of Microorganismsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Influence of different chemical agents and storage conditions on the microbiological content of industrial hemp (Cannabis sativa L.) seeds

Đerić¹,

Todorić²,

Rošul³

et al. 2020

Food & Feed Res

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Occurrence of Deteriorating Seed-Borne Pathogens and Weevils (Callosobruchus Maculatus(f.) Bruchidae Coleoptera) and Their Economic Impacts on Marketing of Stored Cowpea Grains in Maiduguri, Borno State

Richard

Goñi²,

Usman³

et al. 2023

FJS

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The experiment was conducted to investigate the economic impacts of fungi seed-borne pathogens and cowpea grain weevil (Callosobruchus naculatus (Fab.) BRUCHIDAE COLEOPTERA) on stored cowpea grains which include kananado, Biu-Fari, Bornoji, Kirikiri Banjara jaa, Warware-Bashi, Bonjera and Rangen. The experiment was conducted in a completely randomized design (CRD) in the Crop Protection Laboratory, University of Maiduguri. Blotter's paper seed plating and Agar methods were adopted for the purpose of the investigation. Each cowpea sample were surface sterilized with 10% Sodium hypochlorite for 1-2 minutes and rinsed thrice with sterile distilled water. Ten (10) surface sterilized seeds were aseptically plated per moistened Petri-dishes using a sterilized forceps and incubated at temperature of 21±2 0 C for 7 days. Results showed that there were significant differences (P≤0.05) on germination percentage, 1000 seed weight (g) and disease incidence among the Cowpea variety seeds tested. Higher germination percentage were observed among Kanannado and Bornoji, while Wareware-Bashi had the lowest from 24 to 120 hours after plating (HAP) compared to other Cowpea varieties respectively. Biu-Fari significantly recorded the highest 1000 seed weight and the highest disease incidence from 72 to 120 (HAP). Bornoji recorded the lowest disease incidence from 24 to 120 (HAP) compare to other varieties respectively. Fungal isolates which include Aspergillus niger, Aspergillus flavus, Mucor racemosus and Trichoderma harzianum were isolated from the infected cowpea seeds and identified. The isolates colony pure culture on Potato Dextrose Ager (PDA) were greenish (Aspergillus flavus), black to dark brown (Aspergillus niger), whitish (Mucur recemoses) and light yellowish (Trichoderma harzianum) respectively Disease causing pathogens posed a dangerous health threat and perforation inflicted on the grains by the weevils can lower the market value. Hence, proper seed handling, disease monitoring and safe storage environmental conditions become very necessary in order to prevent seed damage and contamination by the fungal pathogens and C. maculatus.

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Management of seed-borne fungi in cowpea using leaf extracts and sodium bicarbonate

Cited by 2 publications

References 15 publications

Influence of different chemical agents and storage conditions on the microbiological content of industrial hemp (Cannabis sativa L.) seeds

Influence of different chemical agents and storage conditions on the microbiological content of industrial hemp (Cannabis sativa L.) seeds

Occurrence of Deteriorating Seed-Borne Pathogens and Weevils (Callosobruchus Maculatus(f.) Bruchidae Coleoptera) and Their Economic Impacts on Marketing of Stored Cowpea Grains in Maiduguri, Borno State

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