Radicular cysts are the most common odontogenic cyst arising from epithelial cell rests of Malassez in the periodontal ligament and proliferate peripherally as a result of inflammation caused by infection at the root apices. They slowly grow apically causing bone resorption and displacement of the adjacent teeth which radiographically is seen as a radiolucent unilocular lesion. Management of such bone defects can be done by using various bone graft substitutes such as autogenous, allografts, xenografts and alloplasts. Taking account of the benefits of using synthetic bone graft substitutes, the following case represents the successful management of a radicular cyst, which includes endodontic treatment and cyst enucleation, chemical cauterization followed by introduction of antibiotic (Vancomycin) releasing calcium sulfate filler beads into the defect.