To compare the clinical effects of subgingival irrigation with ozonated water to the effects of chlorhexidine solution on gingivitis in orthodontic patients and to correlate the clinical effects with the MCP-1 activity in gingival crevicular fluid (GCF). Correlate the inflammatory marker monocyte chemoattractant protein (MCP-1) in GCF with clinical parameters viz. gingival index (GI), plaque index (PI), gingival bleeding index (GBI), probing pocket depth (PPD).