Management of Environmental Phosphorus Pollution Using Phytases: Current Challenges and Future Prospects

Kumar, Vinod; Singh, Dharmendra; Sangwan, Punesh; Gill, Prabhjot Kaur

doi:10.1007/978-81-322-2123-4_7

Cited by 15 publications

(10 citation statements)

References 108 publications

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“…One such antinutritional factor is the phytic acid ( myo -inositol 1,2,3,4,5,6-hexa kis phosphate; IP 6 ) which is the major storage form of phosphorus (60–80 %), in soil (Turner et al 2002 ), grains (Lott et al 2000 ) and manures from monogastric animals (Barnett 1994 ). It acts as an antinutrient as it chelates various micronutrients such as Ca 2+ , Fe 2+ , and Zn 2+ and diminishes their bioavailability in digestive tract of monogastric animals due to lack of enzyme for hydrolysis of phytate complexes (Kumar et al 2014 , 2015 ). Therefore, consumption of food containing large amount of phytate may cause severe mineral deficiency.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, consumption of food containing large amount of phytate may cause severe mineral deficiency. The micronutrient deficiency could be alleviated using exogenous phytase addition (Kumar et al 2015 ), development of phytase secreting transgenic plants (Brinch-Pedersen et al 2000 ), and production of low phytate mutant plants to use in food and feed of the humans (Raboy 2001 ). Addition of phytase during food processing is most preferred as the other two approaches may lead to decreased seed phytate leading to deleterious effect on germination and decreased agricultural field.…”

Section: Introductionmentioning

confidence: 99%

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Phytases from Enterobacter and Serratia species with desirable characteristics for food and feed applications

et al. 2016

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Phytases are enzymes of great industrial importance with wide range of applications in animal and human nutrition. These catalyze the hydrolysis of phosphomonoester bonds in phytate, thereby releasing lower forms of myo-inositol phosphates and inorganic phosphate. Addition of phytase to plant-based foods can improve its nutritional value and increase mineral bioavailability by decreasing nutritional effect of phytate. In the present investigation, 43 phytase positive bacteria on PSM plates were isolated from different sources and characterized for phytase activity. On the basis of phytase activity and zone of hydrolysis, two bacterial isolates (PSB-15 and PSB-45) were selected for further characterization studies, i.e., pH and temperature optima and stability, kinetic properties and effect of modulators. The phytases from both isolates were optimally active at the pH value from 3 to 8 and in the temperature range of 50–70 °C. Further, the stability of isolates was good in the pH range of 3.0–8.0. Much variation was observed in temperature and storage stability, responses of phytases to metal ions and modulators. The K m and V max values for PSB-15 phytase were 0.48 mM and 0.157 μM/min, while for PSB-45 these were 1.25 mM and 0.140 μM/min, respectively. Based on 16S rDNA gene sequence, the isolates were identified as Serratia sp. PSB-15 (GenBank Accession No. KR133277) and Enterobacter cloacae strain PSB-45 (GenBank Accession No. KR133282). The novel phytases from these isolates have multiple characteristics of high thermostability and good phytase activity at desirable range of pH and temperature for their efficient use in food and feed to facilitate hydrolysis of phytate-metal ion complex and in turn, increased bioavailability of important metal ions to monogastric animals.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Phytases from Enterobacter and Serratia species with desirable characteristics for food and feed applications

et al. 2016

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“…Four classes of phytases, namely, (HAPhy), (BPPhy), (PAPhy), and (CPhy), have been reported in the literature based on catalytic and sequence features [98]. These have also been categorized as 3-phytase and 4/6-phytase based on initial site of action and liberation of inorganic phosphate from phytate structure [99]. Several applications of phytases and phytaseproducing microbes have been reported leading to tremendous opportunities in using these microbes for beneficial purposes.…”

Section: Beneficial Microbes For Sustainable Agriculturementioning

confidence: 99%

“…Reducing environmental phosphorus pollution is of great importance in areas of intensive livestock production of monogastric animals, where unavailable phosphate excreted and deposited in nearby water bodies leading to its eutrophication. Excess phosphorus in such places causes algal blooms and death of aquatic life therein [99]. Phytase-producing microbes or neutral phytases have a potential role to be used as a supplement in meals of aquatic animals [101,102].…”

Section: Beneficial Microbes For Sustainable Agriculturementioning

confidence: 99%

Beneficial microbiomes: Biodiversity and potential biotechnological applications for sustainable agriculture and human health

Yadav¹,

Kumar²,

Kumar³

et al. 2017

J App Biol Biotech

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The beneficial microbes plays an important role in medical, industrial, and agricultural processes. The precious microbes belong to different groups including archaea, bacteria, and fungi which can be sort out from different habitat such as extreme environments (acidic, alkaline, drought, pressure, salinity, and temperatures) and associated with plants (epiphytic, endophytic, and rhizospheric) and human. The beneficial microbes exhibited multifunctional plant growth promoting (PGP) attributes such as N 2 -fixation, solubilization of micronutrients (phosphorus, potassium and zinc), and production of siderophores, antagonistic substances, antibiotic, auxin, and gibberellins. These microbes could be applied as biofertilizers for native as well as crops growing at diverse extreme habitat. Microbes with PGP attributes of N 2 -fixation, P-, and K-solubilization could be used at a place of NPK chemical fertilizers. Agriculturally, important microbes with Fe-and Zn-solubilizing attributes can be used for biofortification of micronutrients in different cereal crops. The biofertilizers are an eco-friendly technology and bioresources for sustainable agriculture and human health. In general, the concentrations of micronutrient in different crops are not adequate for human nutrition in diets. Hence, consumption of such cereal-based diet may result in micronutrient malnutrition and related severe health complications. The biofortification approach is getting much attention to increase the availability of micronutrients, especially Fe and Zn in the major food crops. The beneficial microbes can be used as probiotic as functional foods for human health. Probiotics microbes such as Bifidobacterium, Lactobacillus, Methanobrevibacter, Methanosphaera, and Saccharomyces are increasingly being used as dietary supplements in functional food products. The microbes with beneficial properties could be utilized for sustainable agriculture and human health.

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“…The supplementation of diets with microbial phytases increases the bioavailability of phosphorus and micronutrients besides reducing the aquatic phosphorus pollution in the areas of intensive livestock production and combating environmental phosphorus pollution (Kumar et al 2015). Phytases has tremendous economic impact in animal feed market and according to the report of first phytase summit in 2010, phytases share 60% of total feed enzyme market with a global phytase market of $350 million annually (Reddy et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Production and characterization of a neutral phytase of Penicillium oxalicum EUFR-3 isolated from Himalayan region

et al. 2017

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Abstract. Kaur R, Saxena A, Sangwan P, Yadav AN, Kumar V, Dhaliwal HC. 2017. Production and characterization of a neutral phytase of Penicillium oxalicum EUFR-3 isolated from Himalayan region. Nusantara Bioscience 9: 68-76. Micronutrient bioavailability from cereals is poor to monogastric animals due to presence of phytate as a chelating agent in seeds and an exogenous application of phytases is required to overcome antinutritional effect of phytate. In the present investigation, 40 fungal isolates including Aspergillus, Penicillium, Trichoderma, Toliposporium, Cladosporium, Talaromyces etc. from different habitats were screened positive for phytase activity on specific media. On the basis of phytase production potential in solid state fermentation conditions, plant epiphytic fungi Penicillium oxalicum strain EUFR-3 has been observed with maximum phytase activity of 12.8 U/g. The biochemical properties i.e. activity and stability under optimum pH and temperature, and effect of modulators on phytase activity from this isolate were studied for its prospective application in human food. The P. oxalicum EUFR-3 phytase (PhyP-EUFR3) had maximum activity at pH 7.0 and temperature 40 °C. It was stable in pH range of 3-8 with more than 60 % activity throughout investigation. The thermostability of PhyP-EUFR3 was about 30 % residual activities after 10 min at 80 °C. Further purification, characterization and study of catalytic mechanism will be useful in better utilization of this phytase in human food for increased bioavailability of important metal ions.

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Management of Environmental Phosphorus Pollution Using Phytases: Current Challenges and Future Prospects

Cited by 15 publications

References 108 publications

Phytases from Enterobacter and Serratia species with desirable characteristics for food and feed applications

Phytases from Enterobacter and Serratia species with desirable characteristics for food and feed applications

Beneficial microbiomes: Biodiversity and potential biotechnological applications for sustainable agriculture and human health

Production and characterization of a neutral phytase of Penicillium oxalicum EUFR-3 isolated from Himalayan region

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