Management of Drug-Resistant TB

Hmama, Zakaria

doi:10.5772/55531

Cited by 2 publications

(1 citation statement)

References 92 publications

(103 reference statements)

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“…Identification of NTM has been conducted only in a few clinical laboratories in China, so misdiagnosis and incorrect treatment are commonly given to the patients infected with NTM. As some of the NTM species are natively insensitive to the commonly used antituberculosis drugs, the NTM infected patients may be treated as drug-resistant TB, which result in some of the TB patients getting misdiagnosis and inappropriate therapy [8]. Once the anti-TB therapy fails, the treatment of NTM infection might be delayed several months or even more.…”

Section: Introductionmentioning

confidence: 99%

Identification of Species of Nontuberculous Mycobacteria Clinical Isolates from 8 Provinces of China

Liu

Lian

Jiang

et al. 2016

BioMed Research International

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Pulmonary diseases caused by nontuberculous mycobacteria (NTM) are increasing in incidence and prevalence worldwide. In this study, we identified NTM species of the clinical isolates from 8 provinces in China, in order to preliminarily provide some basic scientific data in the different species and distribution of NTM related to pulmonary disease in China. A total of 523 clinical isolates from patients with tuberculosis (TB) diagnosed clinically from 2005 to 2012 were identified to the species using conventional and molecular methods, including multilocus PCR, rpoB and hsp65 PCR-PRA, hsp65, rpoB, and 16S-23S internal transcribed spacer region sequencing. The isolates were identified into 3 bacterium genera, including NTM, Gordonia bronchialis, and Nocardia farcinica, and, for the 488 NTM isolates, 27 species were identified. For all the 27 species of NTM which were found to cause pulmonary infections in humans, the most prevalent species was M. intracellulare, followed by M. avium and M. abscessus. And seven other species were for the first time identified in patients with TB in China. NTM species identification is very important for distinguishing between tuberculosis and NTM pulmonary diseases, and the species diversity drives the creation of diverse and integrated identification methods with higher accuracy and efficacy.

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Section: Introductionmentioning

confidence: 99%

Identification of Species of Nontuberculous Mycobacteria Clinical Isolates from 8 Provinces of China

Liu

Lian

Jiang

et al. 2016

BioMed Research International

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In-vitro Anti-Tuberculosis, Anti-Efflux Pumps and Anti-Biofilm Effects of Crinum Asiaticum Bulbs

Ofori¹,

Danquah²,

Ativui³

et al. 2021

Biomed. Pharmacol. J.

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Drug resistant tuberculosis remains one of the major challenges associated with treatment and management of tuberculosis (TB) in the public health system and in clinical settings. In 2020, the World Health Organization (WHO) estimated that about 186,772 people died from drug-resistant tuberculosis out of the 500000 reported cases and this is alarming. There is a pressing need from every angle in drug discovery to develop novel compounds that could possess diverse mechanisms of action to tackle drug-resistant tuberculosis. The Crinum asiaticum bulbs extract are used ethno medicinally to treat upper respiratory tract infections and as well as wound healing agent. The aim of this work is to investigate the in-vitro anti-tuberculosis effect of Crinum asiaticum bulbs extracts and to assess the inhibitory properties against bacteria efflux pumps expression and biofilm formation. The results obtained showed that the Crinum asiaticum bulbs extracts (CAE) were effective in inhibiting Mycobacterium smegmatis (NCTC 8159) and Mycobacterium aurum (NCTC 10437) with minimum inhibitory concentration (MIC) of 125 μg/ml and 250 μg/ml against M. smegmatis and M. aurum respectively. The CAE markedly inhibited the efflux pumps of both M. smegmatis and M. aurum from expressing with the chloroform extract producing the greatest inhibition. The CAE (ethanol, methanol, chloroform and hexane) significantly (***ρ˂0.005) inhibited M. smegmatis’ and M. aurum’s biofilm formation in-vitro. Among the various extracts of Crinum asiaticum, the chloroform extract exhibited the greatest inhibition against M. smegmatis and M. aurum biofilm formation with significance levels of ***ρ˂0.005 and ***ρ˂0.005. In conclusion the CAE has anti-tuberculosis effect and could tackle drug resistant TB as exhibited through the anti-efflux and anti-biofilm forming properties of the extract against the selected Mycobacterium species.

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Management of Drug-Resistant TB

Cited by 2 publications

References 92 publications

Identification of Species of Nontuberculous Mycobacteria Clinical Isolates from 8 Provinces of China

Identification of Species of Nontuberculous Mycobacteria Clinical Isolates from 8 Provinces of China

In-vitro Anti-Tuberculosis, Anti-Efflux Pumps and Anti-Biofilm Effects of Crinum Asiaticum Bulbs

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