Management of cytomegalovirus infection after liver transplantation

Sampathkumar, Priya; Payá, Carlos V.

doi:10.1002/lt.500060220

Cited by 19 publications

(12 citation statements)

References 80 publications

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“…Human cytomegalovirus (HCMV), a widespread human pathogen (Griffiths and Walter, 2005;Lawson, 2005;Ross and Boppana, 2005), is a prototypic β-herpesvirus, having a long replicative cycle, a restricted host range, and a cytopathic effect resulting in pronounced cell rounding and swelling (Drago et al, 2000;Griffiths and Walter, 2005;Kano and Shiohara, 2000;Sampathkumar and Paya, 2000). Sequence analysis of its ≈ 230-kb genome (Chee et al, 1990a) revealed four open reading frames (ORFs), US27, US28, UL33, and UL78 (Attwood and Findlay, 1994a,b;Chee et al, 1990b), that encode potential homologues of cellular G protein-coupled receptors (GPCRs) (Lomize et al, 1999;Pierce et al, 2002;Pin et al, 2003;Schoneberg et al, 1999;Strange, 1999), a large group of cell surface-exposed signal transducing proteins (Pierce et al, 2002;Pin et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

The chemokine receptor homologue encoded by US27 of human cytomegalovirus is heavily glycosylated and is present in infected human foreskin fibroblasts and enveloped virus particles

Margulies

Gibson

2007

Virus Research

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Human cytomegalovirus (HCMV), a member of the β-herpesvirus family, encodes four homologues of cellular G protein-coupled receptors (GPCRs). One of these, the protein product of HCMV open reading frame (ORF) UL33, has been identified in HCMV-infected cells and virus particles and shown to be heat-aggregatable and N-glycosylated. Another, the product of ORF US28, has been functionally characterized as a β-chemokine receptor. Here we report the use of RT-PCR, coupled in vitro transcription-translation, immunoprecipitation, and Western immunoassays to (i) show that RNA from the open reading frame US27 appears predominantly during the late phase of replication; (ii) identify the protein encoded by HCMV US27 in infected cells and enveloped virus particles; (iii) demonstrate that the US27-encoded protein is heterogeneously N-glycosylated and resolves as two species following treatment with peptide N-glycosidase F; and (iv) show that both the recombinant and deglycoylated infected cell US27 protein aggregate when heated in the presence of SDS prior to electrophoresis in polyacrylamide gels, a property which is abrogated with the addition of urea to sample buffer.

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Section: Introductionmentioning

confidence: 99%

The chemokine receptor homologue encoded by US27 of human cytomegalovirus is heavily glycosylated and is present in infected human foreskin fibroblasts and enveloped virus particles

Margulies

Gibson

2007

Virus Research

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“…Among the complications caused by CMV in transplanted patients increased long-term mortality and worsening graft survival are common [35][36][37]. Clinical disease caused by CMV is expressed by fever, malaise, myalgia, leukopenia (WBC less than 4.0000/mm 3 ), increased transaminases (hepatitis), pulmonary (pneumonitis) and/or gastrointestinal (colitis, gastritis, esophagitis) and fever being the most common manifestation, which can also occur with neurological symptomatology compatible with encephalitis but these are rarer [29,38,39].…”

Section: Clinical Manifestationsmentioning

confidence: 99%

“…During liver transplantation, primary infection tends to be more important as the CMV viremia may be limited to when virus replication is detected in peripheral blood or significant increase of specific antibodies without symptoms or viral syndrome presenting fever equal or greater than 38ºC, malaise, leukopenia, atypical lymphocytosis equal or less than 3-5% and thrombocytopenia [14,37,42,45,46]. Antiviral treatment controls the acute manifestation of the disease in most cases, but may not eradicate the CMV with recurrence reported in 26-31% of solid organ transplant recipients.. [14,36].…”

Section: Secondary Infection Occurs When the Reactivation Of Latent Imentioning

confidence: 99%

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Cytomegalovirus Infection in Liver Transplantation

Sampaio¹,

Guardia²,

Milan³

et al. 2013

Manifestations of Cytomegalovirus Infection

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“…CMV primary infection results in life-long residence of the virus in the host, and reactivates in immunocompromised individuals frequently. Reactivation of CMV infection and development of related severe diseases and syndromes are common in solid organ recipients may lead to severe complications following transplant, such as acute rejection [5,6] . CMV may lead also to higher rates of bacterial and fungal infections in transplant recipients [7] .…”

Section: Introductionmentioning

confidence: 99%

Role of cytomegalovirus on the maturation and function of monocyte derived dendritic cells of liver transplant patients

Karimi

Shariat

Yaghobi

et al. 2016

WJT

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AIM:To study the impact of association between cytomegalovirus (CMV) pathogenesis with dendritic cell (DC) maturation and function was evaluated in CMV reactivated liver transplanted patients in comparing with non-reactivated ones, and healthy controls. METHODS:Monocyte derived dendritic cells (MoDCs) was generated from collected ethylenediaminetetraacetic acid-treated blood samples from patient groups and controls. In these groups, expression rates and mean fluorescent intensity of DC markers were evaluated using flowcytometry technique. Secretion of cytokines including: interleukin (IL)-6, IL-12 and IL-23 were determined using enzyme-linked immunosorbent assay methods. The gene expression of toll-like receptor 2 (TLR2), TLR4 and IL-23 were analyzed using in-house real-time polymerase chain reaction protocols. RESULTS:Results have been shown significant decreases in: Expression rates of MoDC markers including CD83, CD1a and human leukocyte antigen DR (HLA-DR), the mean fluorescence intensitys for CD1a and HLA-DR, and secretion of IL-12 in CMV reactivated compared CONCLUSION: DC functional defects in CMV reactivated recipients, such as decrease in expression of DC maturation markers, increase in secretion of proinflammatory cytokines, and TLRs can emphasize on the importance of CMV infectivity in development of liver rejection in transplanted patients.

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Management of cytomegalovirus infection after liver transplantation

Cited by 19 publications

References 80 publications

The chemokine receptor homologue encoded by US27 of human cytomegalovirus is heavily glycosylated and is present in infected human foreskin fibroblasts and enveloped virus particles

The chemokine receptor homologue encoded by US27 of human cytomegalovirus is heavily glycosylated and is present in infected human foreskin fibroblasts and enveloped virus particles

Cytomegalovirus Infection in Liver Transplantation

Role of cytomegalovirus on the maturation and function of monocyte derived dendritic cells of liver transplant patients

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