The study investigates the effect of land-use change on nitrous oxide (N 2 O) and methane (CH 4 ) fluxes from soil, in savanna ecosystems of the Orinoco region (Venezuela). Gas fluxes were measured by closed static chambers, in the wet and dry season, in representative systems of land management of the region: a cultivated pasture, an herbaceous savanna, a tree savanna and a woodland (control site). Higher N 2 O emissions were observed in the cultivated pasture and in the herbaceous savanna compared with the tree savanna and the woodland, and differences were mainly related to fine soil particle content and soil volumetric water content measured in the studied sites. Overall N 2 O emissions were quite low in all sites (0-1.58 mg N 2 O-N m À2 day À1 ). The cultivated pasture and the woodland savanna were on average weak CH 4 sinks (À0.05 AE 0.07 and À0.08 AE 0.05 mg CH 4 m À2 day À1 , respectively), whereas the herbaceous savanna and the tree savanna showed net CH 4 production (0.23 AE 0.05 and 0.19 AE 0.05 mg CH 4 m À2 day À1 , respectively). Variations of CH 4 fluxes were mainly driven by variation of soil waterfilled pore space (WFPS), and a shift from net CH 4 consumption to net CH 4 production was observed at around 30% WFPS. Overall, the data suggest that conversion of woodland savanna to managed landscape could alter both CH 4 and N 2 O fluxes; however, the magnitude of such variation depends on the soil characteristics and on the type of land management before conversion.