Man as a living bioreactor: Long-term histological aspects of a mandibular replacement engineered in the patient’s own body

Naujokat, Hendrik; Açil, Yahya; Gülses, Aydın; Birkenfeld, Falk; Wiltfang, Jens

doi:10.1016/j.ijom.2018.05.006

Cited by 25 publications

(28 citation statements)

References 24 publications

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“…None of the study cited in (six studies in total), searching “Human body as living bioreactor” introduced the concept of “Engineered fat graft”, in which the human body, and in particular the breast, could be considered as a bioreactor for fat grafting injected, favoring neo-angiogenesis that is promoted by SVFs-ASCs, contained in EF-e-A and growth factors release. Warnke et al introduced the concept of “Man as living bioreactor” for the first time by [31] in 2006, and after by Naujokat et al [32] (2018) and Wiltfang et al [33] (2016). In detail, Warnke et al [31] reported a novel method of repairing a human mandible by in vivo tissue engineering, in which the patients served as his own bioreactor as the exogenously prepared customized mandible replacement was grown inside his latissimus dorsi muscle prior to transplantation to repair the existing defect.…”

Section: Discussionmentioning

confidence: 99%

“…In fact, ASCs secrete pro-angiogenic factors, such as Vascular Endothelial Growth Factor (VEGF), associate perivascularly with blood vessels, and provide physical Extracellular Matrix (ECM) guidance cues that promote endothelial sprouting [28,29,30]. The concept of “Man as living bioreactor”, was introduced for the first time by Warnke et al [31] in 2006, and after by Naujokat et al [32] (2018) and Wiltfang et al [33] (2016). For the first time, the authors reported, in the present study, the concept of bioreactor as applied to the fat graft and at the breast.…”

Section: Introductionmentioning

confidence: 99%

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Engineered Fat Graft Enhanced with Adipose-Derived Stromal Vascular Fraction Cells for Regenerative Medicine: Clinical, Histological and Instrumental Evaluation in Breast Reconstruction

Gentile

Casella

Palma

et al. 2019

JCM

100

113

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The areas in which Stromal Vascular Fraction cells (SVFs) have been used include radiotherapy based tissue damage after mastectomy, breast augmentation, calvarial defects, Crohn’s fistulas, and damaged skeletal muscle. Currently, the authors present their experience using regenerative cell therapy in breast reconstruction. The goal of this study was to evaluate the safety and efficacy of the use of Engineered Fat Graft Enhanced with Adipose-derived Stromal Vascular Fraction cells (EF-e-A) in breast reconstruction. 121 patients that were affected by the outcomes of breast oncoplastic surgery were treated with EF-e-A, comparing the results with the control group (n = 50) treated with not enhanced fat graft (EF-ne-A). The preoperative evaluation included a complete clinical examination, a photographic assessment, biopsy, magnetic resonance (MRI) of the soft tissue, and ultrasound (US). Postoperative follow-up took place at two, seven, 15, 21, 36 weeks, and then annually. In 72.8% (n = 88) of breast reconstruction treated with EF-e-A, we observed a restoration of the breast contour and an increase of 12.8 mm in the three-dimensional volume after 12 weeks, which was only observed in 27.3% (n = 33) of patients in the control group that was treated with EF-ne-A. Transplanted fat tissue reabsorption was analyzed with instrumental MRI and US. Volumetric persistence in the study group was higher (70.8%) than that in the control group (41.4%) (p < 0.0001 vs. control group). The use of EF-e-A was safe and effective in this series of treated cases.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Engineered Fat Graft Enhanced with Adipose-Derived Stromal Vascular Fraction Cells for Regenerative Medicine: Clinical, Histological and Instrumental Evaluation in Breast Reconstruction

Gentile

Casella

Palma

et al. 2019

JCM

100

113

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“…Heatmap representing the proportion of publications by year that utilized specific translational research methodologies from construct characterization to human trial to investigate craniofacial tissue engineering 49‐72,74‐77,79,81‐84,145‐197 …”

Section: Discussionmentioning

confidence: 99%

“…In vivo bioreactors have been utilized to create autologous bone flaps for craniofacial defects 80 . The vascularization and precursor cells present in these bioreactors allow for larger bone constructs to be engineered 81 . The first human case of mandibular reconstruction using the greater omentum as a bioreactor was reported in 2016 82 .…”

Section: Discussionmentioning

confidence: 99%

“…The first human case of mandibular reconstruction using the greater omentum as a bioreactor was reported in 2016 82 . A follow‐up investigation after 10 months showed that the amount of vital mineralized bone tissue of the graft in the mandible had continued to increase 81 . A morselized bone autograft was utilized as the scaffold in an in vivo bioreactor study that successfully replaced angle of mandible defects in sheep 83 .…”

Section: Discussionmentioning

confidence: 99%

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Tissue engineering applications in otolaryngology—The state of translation

Niermeyer

Rodman

et al. 2020

Laryngoscope Investig Oto

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While tissue engineering holds significant potential to address current limitations in reconstructive surgery of the head and neck, few constructs have made their way into routine clinical use. In this review, we aim to appraise the state of head and neck tissue engineering over the past five years, with a specific focus on otologic, nasal, craniofacial bone, and laryngotracheal applications. A comprehensive scoping search of the PubMed database was performed and over 2000 article hits were returned with 290 articles included in the final review. These publications have addressed the hallmark characteristics of tissue engineering (cellular source, scaffold, and growth signaling) for head and neck anatomical sites. While there have been promising reports of effective tissue engineered interventions in small groups of human patients, the majority of research remains constrained to in vitro and in vivo studies aimed at furthering the understanding of the biological processes involved in tissue engineering. Further, differences in functional and cosmetic properties of the ear, nose, airway, and craniofacial bone affect the emphasis of investigation at each site. While otolaryngologists currently play a role in tissue engineering translational research, continued multidisciplinary efforts will likely be required to push the state of translation towards tissue‐engineered constructs available for routine clinical use. Level of Evidence NA.

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Advances in bioreactors for lung bioengineering: From scalable cell culture to tissue growth monitoring

Mahfouzi

Amoabediny

Tali

2021

Biotech & Bioengineering

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Man as a living bioreactor: Long-term histological aspects of a mandibular replacement engineered in the patient’s own body

Cited by 25 publications

References 24 publications

Engineered Fat Graft Enhanced with Adipose-Derived Stromal Vascular Fraction Cells for Regenerative Medicine: Clinical, Histological and Instrumental Evaluation in Breast Reconstruction

Engineered Fat Graft Enhanced with Adipose-Derived Stromal Vascular Fraction Cells for Regenerative Medicine: Clinical, Histological and Instrumental Evaluation in Breast Reconstruction

Tissue engineering applications in otolaryngology—The state of translation

Advances in bioreactors for lung bioengineering: From scalable cell culture to tissue growth monitoring

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