Mammary stem cells: expansion and animal productivity

Choudhary, Ratan K.

doi:10.1186/2049-1891-5-36

Cited by 13 publications

(4 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Drying-off has been widely recognized as a critical period of the production cycle of dairy cows (Capuco and Akers, 1999;Bachman and Schairer, 2003;Choudhary, 2014). It has been suggested that with increasing milk yields over the last decades, the transition from lactating to dry has progressively become more challenging for cows in terms of animal health and welfare (Thornton, 2010;Zobel et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Effects of oral administration of acidogenic boluses at dry-off on performance and behavior of dairy cattle

Maynou

Elcoso²,

Bubeck

et al. 2018

Journal of Dairy Science

View full text Add to dashboard Cite

The aim of this study was to evaluate the potential of oral acidogenic mineral boluses (196 g) containing anionic salts to facilitate the transition from lactation to the dry stage by inducing a mild and temporary metabolic acidosis at dry-off. In experiment 1, 84 lactating cows were randomly allocated to 1 of 3 treatment groups consisting of an oral administration of 0, 1, or 2 boluses 5 d before dry-off to evaluate the effects on milk production. In experiment 2, 16 lactating cows were involved in a crossover study to evaluate the effects of the administration of 2 boluses on milk production, feed intake, and urine pH. In experiment 3, 152 lactating cows were allocated to 1 of 2 treatments (control: no treatment; bolus: 2 oral boluses the day before last milking) to evaluate udder pressure, incidence of milk leakage, and lying behavior during the first days following dry-off. Also, milk yield in the subsequent lactation for all enrolled cows was recorded during the first 60 DIM. In experiment 1, cows receiving 2 boluses had the greatest reduction in milk production (-2.56 kg/d of milk) compared with those receiving 1 bolus or no treatment (-1.15 and -0.23 kg/d, respectively) the second day after bolus application. In experiment 2, the application of oral boluses decreased feed intake of cows during the first 3 d following treatment, and milk production was reduced on d 2 and 3 after bolus application. Reduced urine pH at 8 and 24 h after treatment was observed in bolus cows compared with control cows. In experiment 3, bolus cows had lower udder pressure after drying off, but incidence of milk leakage did not differ between treatments. Bolus cows had an additional 85 min of lying time in the 24 h following dry-off. Serum P and β-OH-butyrate concentrations were lower in bolus cows than in control cows after dry-off, but no other differences in blood parameters between treatments were observed. Also, no differences in milk yield in the subsequent lactation were observed between treatments. It is concluded that oral bolus application diminishes feed intake and milk production, and, if applied at dry-off, it decreases udder pressure and increases lying time during the first 24 h after dry-off.

show abstract

Section: Introductionmentioning

confidence: 99%

Effects of oral administration of acidogenic boluses at dry-off on performance and behavior of dairy cattle

Maynou

Elcoso²,

Bubeck

et al. 2018

Journal of Dairy Science

View full text Add to dashboard Cite

show abstract

“…Among the 96 compounds, Xanthosine showed the highest peak area (15.79%). Xanthosine was found to have wound healing, antibacterial property, and also have application in stem cell research (Choudhary, 2014). The other compounds having comparatively high peak area were naphthalene (5.39%), 9,9-Perhydrobiphenanthryl (4.75%), 4-((1e)-3-hydroxy-1propenyl)-2-methoxyphenol (4.54%), 3,6,9-triethyl-3,6,9trimethyl tetracyclo[6.1.0.0~2,4~.0~5,7~]nonane (3.12%), and the remaining showed less than 3% of peak area.…”

Section: Resultsmentioning

confidence: 99%

Preliminary phytochemical, microscopic analysis and metabolite profiling of Phoenix pusilla root

2020

J app pharm sci

View full text Add to dashboard Cite

Palms are considered as familiar strangers. Various studies reported that the genus Phoenix was mostly cultivated, traditionally used plants as medicine, and also as an ornamental plant. In this present study, preliminary phytochemical analysis, the study of microscopical structures and the chemical constituents of Phoenix pusilla root was evaluated. Transverse Section of P. pusilla root was studied by sectioning the two different thickness roots and also powder microscopy was carried out. Non-polar, volatile constituents of crude ethanolic extract of root were analyzed by GC-MS. Primary and secondary metabolites quantification revealed that the carbohydrate content and total phenolics concentration were higher than the other metabolites. The physicochemical analysis showed the moisture content was 3.78 ± 0.54, the ash content value was 2.46 ± 0.95, and the crude fiber content was higher than protein, fat content. Microscopy study showed the presence of unique tracheids. Metabolite profiling revealed the presence of 96 different constituents and more than 10 found to have biological importance. Pharmacognostic study is an important step of research which reveals about shelf-life of the drug, adulterations, etc.

show abstract

“…Histomorphological features of poorly differentiated MEC can be observed by visualizing cellular morphology wherein the cells are smaller in size, absence of fat vacuole inside the cytoplasm and reduced cytoplasmic area. 17,18 In mammary carcinomas, aberrant expression of MUC1 was seen. MUC1 was located in the nuclei plus cytoplasm of majority of the MEC ( Figure 4C).…”

Section: Immunolocalization Of Muc1 and Ki67mentioning

confidence: 99%

Mucin 1 Aberrantly Expresses in Goat Mammary Carcinoma

Choudhary

Verma

et al. 2017

JSRT

Self Cite

View full text Add to dashboard Cite

Abbreviations: MUC1, mucin1; qPCR, quantitative polymerase chain reaction; ECM, extra-cellular matrix; PSR, picrosirium red; RT-PCR, reverse transcriptase polymerase chain reaction; MEC, mammary epithelial cells; H&E, hematoxylin and eosin; Ki67, marker of proliferation ki-67; FNDC3B, fibronectin type iii domain containing 3b; CCNB, ccyclin B1; MSI1, musashi 1; PCNA, proliferating cell nuclear antigen IntroductionIncidence of mammary cancer in ruminant is rare. The exact reasons of low incidence of mammary cancer in ruminant are not known. The reason of low incidence of mammary cancer in ruminant is likely due to high rate of pregnancies.1 Hormones like estrogen, play an important role in hyperplasia and neoplasia of mammary tissues and increased parity shortens exposure of estrogen and thus reduce incidence of cancer in goats. In addition to this, other factors such as vegetarian diet with high fiber content, short lifespan of animals, continuous milking of the glands and other factors like mammary stem cells. A survey of screening cancers in goats conducted in Africa and none of the cancer were identified to be the mammary cancer. 2A number of studies proposed stem cells to be the origin of breast cancer 3-5 and these stem cells are dynamic rather than static where progesterone hormone targets mammary stem cells in transforming breast cancer.6 This could be the reason that cancer stem cells share gene signatures with mammary stem cells.7 Incidence of mammary tumors in dogs and cats is very high. In fact, 50% of tumors in dogs are mammary tumors almost three times higher than in woman. The reasons of high incidence of mammary cancer in dogs, cats and human could be dietary habits (meat eater) and high intake of carcinogens through diet as they are higher on food chain.1 Therefore, it deserves attention towards medical perspective as how mammary glands of ruminants (goat) are protected from being cancerous.Mucin 1 (MUC1) encodes for a membrane-bound protein that has essential role in providing protective mucosal barrier and cell signaling. O-glycosylated mucin protein has two terminals, N-terminal alpha subunit (MUC1-N) and C-terminal beta subunit (MUC1-C), former being involved in cell adhesion and later one involved in cell signaling. MUC1 protein is expressed by luminal epithelial cells of mammary tissue on their apical surfaces and provides protective barriers against pathogens. MUC1-N terminal and MUC1-C terminal forms heterodimer and expresses on apical border of mammary epithelial cells. Due to loss of cell polarity, during transformation of normal cell to cancer cell, MUC1-C monomer is positioned entire over the cell membrane. Thus, overexpression and aberrant localization of MUC1-C has been associated with mammary cancer. In general, more than 90% of the mammary cancers overexpress MUC1 proteins. 8However, heterogeneity of MUC1 expression has been seen in 14 breast carcinoma cell lines.9 Variability in amount of MUC1 in breast cancer ranges from high to low expression. High cytoplasmic expression and ab...

show abstract

Mammary stem cells: expansion and animal productivity

Cited by 13 publications

References 70 publications

Effects of oral administration of acidogenic boluses at dry-off on performance and behavior of dairy cattle

Effects of oral administration of acidogenic boluses at dry-off on performance and behavior of dairy cattle

Preliminary phytochemical, microscopic analysis and metabolite profiling of Phoenix pusilla root

Mucin 1 Aberrantly Expresses in Goat Mammary Carcinoma

Contact Info

Product

Resources

About